The effect of repeated freezing and thawing on the suture pull‐out strength in equine arytenoid and cricoid cartilages

Gray, Sarah M.; Gutierrez‐Nibeyro, Santiago D.; Horn, Gavin P.; McCoy, Annette M.; Schaeffer, David J.; Stewart, Matt C.

doi:10.1111/vsu.13855

Cited by 2 publications

(1 citation statement)

References 31 publications

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“…Limitations of this study include the basis in cadavers harvested from clinical patients, one-way crossover design, and lack of blinding between the experimental and CFD portions. While freezing cadaver larynges has been demonstrated to have minimal effects on certain biomechanical aspects of laryngeal testing ( 37 ), the effects on airflow remain unknown. Larynges were collected from the hospital population which was quite varied but necessitated by the sources available within financial and geographical reason.…”

Section: Discussionmentioning

confidence: 99%

Computational fluid dynamic analysis of upper airway procedures in equine larynges

et al. 2023

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IntroductionComputational fluid dynamics (CFD) has proven useful in the planning of upper airway surgery in humans, where it is used to anticipate the influence of the surgical procedures on post-operative airflow. This technology has only been reported twice in an equine model, with a limited scope of airflow mechanics situations examined. The reported study sought to widen this application to the variety of procedures used to treat equine recurrent laryngeal neuropathy (RLN). The first objective of this study was to generate a CFD model of an ex-vivo box model of ten different equine larynges replicating RLN and four therapeutic surgeries to compare the calculated impedance between these procedures for each larynx. The second objective was to determine the accuracy between a CFD model and measured airflow characteristics in equine larynges. The last objective was to explore the anatomic distribution of changes in pressure, velocity, and turbulent kinetic energy associated with the disease (RLN) and each surgical procedure performed.MethodsTen equine cadaveric larynges underwent inhalation airflow testing in an instrumented box while undergoing a concurrent computed tomographic (CT) exam. The pressure upstream and downstream (outlet) were measured simultaneously. CT image segmentation was performed to generate stereolithography files, which underwent CFD analysis using the experimentally measured outlet pressure. The ranked procedural order and calculated laryngeal impedance were compared to the experimentally obtained values.Results and discussionThe CFD model agreed with the measured results in predicting the procedure resulting in the lowest post-operative impedance in 9/10 larynges. Numerically, the CFD calculated laryngeal impedance was approximately 0.7 times that of the measured calculation. Low pressure and high velocity were observed around regions of tissue protrusion within the lumen of the larynx. RLN, the corniculectomy and partial arytenoidectomy surgical procedures exhibited low pressure troughs and high velocity peaks compared to the laryngoplasty and combined laryngoplasty/corniculectomy procedures. CFD modeling of the equine larynx reliably calculated the lowest impedance of the different surgical procedures. Future development of the CFD technique to this application may improve numerical accuracy and is recommended prior to consideration for use in patients.

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Section: Discussionmentioning

confidence: 99%

Computational fluid dynamic analysis of upper airway procedures in equine larynges

et al. 2023

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The effect of repeated freezing and thawing on the suture pull‐out strength in equine arytenoid and cricoid cartilages

et al. 2022

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Objective To assess the effect of repeated freezing and thawing on the suture pull‐out strength in arytenoid and cricoid cartilages subjected to the laryngoplasty (LP) procedure. Study design Ex vivo experimental study. Sample population Ten grossly normal equine cadaveric larynges. Methods Bilateral LP constructs were created using a standard LP technique. One hemilarynx was randomly allocated to the single freeze and thaw group and the other allocated to the repeated freeze and thaw (3 complete cycles) group. The suture ends of each LP construct were attached to a load frame and subjected to monotonic loading until construct failure. Mean load (N) and displacement (mm) at LP construct failure were compared between groups. Results All LP constructs failed by suture pull through the arytenoid cartilage. The mean load at failure was similar between groups (118.9 ± 25.5 N in the single freeze and thaw group and 113.4 ± 20.5 N in the repeated freeze and thaw group, P = .62). The mean displacement at failure was similar between groups (54.4 ± 15.1 mm in the single freeze and thaw group and 54.4 ± 15.4 mm in the repeated freeze and thaw group, P = .99). Conclusion Repeated freezing and thawing did not affect the suture pullout strength of the arytenoid and cricoid cartilages. Clinical significance Laryngeal specimens that have been subjected to repeated freezing and thawing can be utilized in the experimental evaluation of LP procedures because there is no alteration of the suture pull‐out strength of the relevant cartilages.

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The effect of repeated freezing and thawing on the suture pull‐out strength in equine arytenoid and cricoid cartilages

Cited by 2 publications

References 31 publications

Computational fluid dynamic analysis of upper airway procedures in equine larynges

Computational fluid dynamic analysis of upper airway procedures in equine larynges

The effect of repeated freezing and thawing on the suture pull‐out strength in equine arytenoid and cricoid cartilages

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