The effect of Raphanus sativus L. seeds on regulation of intestinal motility in rats consuming a high-calorie diet

Yang, Guangming; Bai, Chen; Liu, Tiegang; He, Jianzhen; Gu, Xiaohong

doi:10.1016/j.biopha.2021.111705

Cited by 6 publications

(4 citation statements)

References 41 publications

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“…Previous studies have reported that diet has a significant impact on the gut microbiota and immune responses in inflammatory diseases 32–34 . Our preliminary experimental research found that short‐term consumption of a high‐calorie diet may result in intestinal motility dysfunction and reduced intestinal motility 35 . More importantly, our clinical studies found that a high‐calorie diet is closely associated with recurrent respiratory infections in children 36 .…”

Section: Discussionsupporting

confidence: 52%

“…[32][33][34] Our preliminary experimental research found that short-term consumption of a high-calorie diet may result in intestinal motility dysfunction and reduced intestinal motility. 35 More importantly, our clinical studies found that a high-calorie diet is closely associated with recurrent respiratory infections in children. 36 Therefore, we investigated whether a high-calorie diet could affect the pulmonary inflammatory response and its related mechanisms in juvenile rats with LPS-induced pneumonia.…”

Section: Discussionmentioning

confidence: 73%

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A high-calorie diet aggravates LPS-induced pneumonia by disturbing the gut microbiota and Th17/Treg balance

Liu

Bai

Xian

et al. 2022

Journal of Leukocyte Biology

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The intestinal flora plays an important role in the inflammatory response to the systemic or local infections in the host. A high-calorie diet has been shown to aggravate pneumonia and delay recovery, especially in children. However, the underlying mechanisms remain unclear. Our previous studies demonstrated that a high-calorie diet and LPS atomization synergistically promoted lung inflammation injury in juvenile rats. In this study, specific pathogen-free juvenile rats were placed in a routine environment, and subjected to a high-calorie diet or LPS atomization in isolation as well as combination. Our data revealed that LPS nebulization combined with a high-calorie diet resulted in significant changes in rats, such as slow weight gain, increased lung index, and aggravated lung inflammatory damage. Meanwhile, we found that the aggravation of LPS-induced pneumonia by a high-calorie diet disturbs the balance of Th17/Treg cells. Furthermore, high-throughput sequencing of intestinal contents revealed that a high-calorie diet changed the gut microbiome composition, decreased microbial diversity, and particularly reduced the abundance of the intestinal microbiota associated with the production of short-chain fatty acids (SCFAs) in rats. Consequently, the levels of SCFAs, especially acetate, propionate, and butyrate, were significantly decreased following the intervention of a high-calorie diet. More critically, the effects of a high-calorie diet were shown to be transmissible among pneumonia rats through cohousing microbiota transplantation. Taken together, we provide evidence to support that a high-calorie diet can potentially reset the gut microbiome and metabolites, disrupt Th17/Treg cell balance and immune homeostasis, and aggravate LPS-induced lung inflammatory damage, which may provide a new perspective on the pathogenesis of lung inflammation injury, and suggest a novel microbiota-targeting therapy for inflammatory lung diseases.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 73%

A high-calorie diet aggravates LPS-induced pneumonia by disturbing the gut microbiota and Th17/Treg balance

Liu

Bai

Xian

et al. 2022

Journal of Leukocyte Biology

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“…RS, a member of the Brassicaceae family, contains various chemical components, such as glucosinolates and sulfur‐containing derivatives 17 . Certain active ingredients found in RS seeds exhibit substantial biological activity without accompanying side effects 18 . Through contemporary clinical and pharmacological research, RS has emerged as being particularly effective in alleviating abdominal distension and pain attributed to qi stagnation.…”

Section: Introductionmentioning

confidence: 99%

Identifying the active compounds and mechanism of action of TongFu XieXia Decoction for treating intestinal obstruction using network pharmacology combined with ultra‐high performance liquid chromatography‐quadrupole‐orbitrap mass spectrometry

Ma,

Wu,

Luo

et al. 2023

Rapid Comm Mass Spectrometry

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RationaleTongFu XieXia Decoction (TFXXD), a formulation rooted in traditional Chinese medicine and optimized through clinical practice, serves as an advanced version of the classic Da Cheng Qi decoction used for treating intestinal obstruction (IO), demonstrating significant therapeutic efficacy. However, due to the intricate nature of herbal compositions, the principal constituents and potential mechanisms of TFXXD have yet to be clarified. Accordingly, this study seeks to identify the active compounds and molecular targets of TFXXD, as well as to elucidate its anti‐IO mechanisms.MethodsQualitative identification of the principal constituents of TFXXD was accomplished using ultra‐high preformance liquid chromatography‐quadrupole‐orbitrap mass spectrometry (UPLC‐Q‐Orbitrap‐MS/MS) analysis. PharmMapper facilitated the prediction of potential molecular targets, whereas protein–protein interaction analysis was conducted using STRING 11.0. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed using the Metascape database. A “compounds‐target‐pathway” network was meticulously constructed within Cytoscape 3.8.2. Finally, molecular docking studies were performed to investigate the interactions between the core target and the crucial compound.ResultsUPLC‐Q‐Orbitrap‐MS/MS analysis identified 65 components with high precision and sensitivity. Furthermore, 64 potential targets were identified as integral to TFXXD bioactivity in IO treatment. Gene Ontology enrichment analysis revealed 995 distinct biological functions, while the Kyoto Encyclopedia of Genes and Genomes enrichment analysis identified 143 intricate signaling pathways.ConclusionMolecular docking studies substantiated the substantial affinity between the TFXXD bioactive constituents and their corresponding targets in the context of IO. TFXXD exerts its therapeutic efficacy in IO through a multifaceted interplay between multiple compounds, targets, and pathways. The integration of network pharmacology with UPLC‐Q‐Orbitrap‐MS/MS has emerged as a promising strategy to unravel the intricate web of molecular interactions underlying herbal medicine. However, it is imperative to emphasize the necessity for further in vivo and in vitro experiments.

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“…Raphani Semen contains an extensive variety of pharmaceutically active compounds [11]. In fact, advanced information about the pharmacological role of Raphani Semen in relation to several gastrointestinal diseases has been revealed in a variety of animal experiment models, e.g., ulcerative colitis, intestinal motility, and colon cancer [12,13]. In addition, Raphani Semen and its active compounds have been shown to have potential for antioxidant and anti-inflammatory activity [14,15].…”

Section: Introductionmentioning

confidence: 99%

Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis

et al. 2021

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In this study, we investigated the pharmacological effect of a water extract of Raphani Semen (RSWE) on alcoholic fatty liver disease (AFLD) using ethanol-induced AFLD mice (the NIAAA model) and palmitic acid (PA)-induced steatosis HepG2 cells. An RSWE supplement improved serum and hepatic triglyceride (TG) levels of AFLD mice, as well as their liver histological structure. To explore the molecular action of RSWE in the improvement of AFLD, we investigated the effect of RSWE on four major pathways for lipid homeostasis in the liver: free fatty acid transport, lipogenesis, lipolysis, and β-oxidation. Importantly, RSWE decreased the mRNA expression of de novo lipogenesis-related genes, such as Srebf1, Cebpa, Pparg, and Lpin1, as well as the protein levels of these factors, in the liver of AFLD mice. That these actions of RSWE affect lipogenesis was confirmed using PA-induced steatosis HepG2 cells. Overall, our findings suggest that RSWE has the potential for improvement of AFLD by inhibiting de novo lipogenesis.

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The effect of Raphanus sativus L. seeds on regulation of intestinal motility in rats consuming a high-calorie diet

Cited by 6 publications

References 41 publications

A high-calorie diet aggravates LPS-induced pneumonia by disturbing the gut microbiota and Th17/Treg balance

A high-calorie diet aggravates LPS-induced pneumonia by disturbing the gut microbiota and Th17/Treg balance

Identifying the active compounds and mechanism of action of TongFu XieXia Decoction for treating intestinal obstruction using network pharmacology combined with ultra‐high performance liquid chromatography‐quadrupole‐orbitrap mass spectrometry

Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis

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