Raphani Semen (Raphanus sativus L.) Ameliorates Alcoholic Fatty Liver Disease by Regulating De Novo Lipogenesis

Abstract

In this study, we investigated the pharmacological effect of a water extract of Raphani Semen (RSWE) on alcoholic fatty liver disease (AFLD) using ethanol-induced AFLD mice (the NIAAA model) and palmitic acid (PA)-induced steatosis HepG2 cells. An RSWE supplement improved serum and hepatic triglyceride (TG) levels of AFLD mice, as well as their liver histological structure. To explore the molecular action of RSWE in the improvement of AFLD, we investigated the effect of RSWE on four major pathways for lipid homeostasis in the liver: free fatty acid transport, lipogenesis, lipolysis, and β-oxidation. Importantly, RSWE decreased the mRNA expression of de novo lipogenesis-related genes, such as Srebf1, Cebpa, Pparg, and Lpin1, as well as the protein levels of these factors, in the liver of AFLD mice. That these actions of RSWE affect lipogenesis was confirmed using PA-induced steatosis HepG2 cells. Overall, our findings suggest that RSWE has the potential for improvement of AFLD by inhibiting de novo lipogenesis.

Inhibition of Abelson Tyrosine-Protein Kinase 2 Suppresses the Development of Alcohol-Associated Liver Disease by Decreasing PPARgamma Expression

“…In addition to being up-regulated in alcohol-related cirrhosis patients, 32 PPARγ and many of its target genes have been shown to be up-regulated in the liver in response to alcohol in murine models of alcoholism. 33 , 34 , 35 Most notably, the hepatic deletion of Pparg itself has been shown to attenuate alcohol-induced steatosis and inflammation. 36 In concordance with these findings, Western blot analysis of liver tissue from AH patients revealed significantly higher levels of PPARγ protein expression when compared with healthy controls ( Figure 5 D ).…”

Appearance and Chemical Characteristics of Seeds of Radish with Different Skin Colors

“…This diversity is mainly reflected in a series of insertion or deletion events, the difference in a single nucleotide in a DNA sequence, and units present in a DNA sequence (Kim et al, 2021). Raphani Semen as a dried and mature seed of radishes has various medicinal uses, such as regulation of fatty liver (Park et al, 2021), hypertension (Li et al, 2015), and intestinal motility (Yang et al, 2021), and is listed in Pharmacopoeia of the People’s Republic of China (Chinese Pharmacopoeia Commission, 2020). Raphani Semen from different types of radish varieties and cultivars may also have lots of diversity.…”