The effect of protease on growth performance, nutrient digestibility, and expression of growth-related genes and amino acid transporters in broilers

Abstract

During the course of this trial, our team assessed the influence of protease upon the growth performance, the nutrient digestibility, and the expression of growth-related genes and amino acid transporters within the liver, muscle, and small intestines of broilers. During the first step, our team allocated 600 broilers into four dietary treatments for a period of 35 days in order to measure the growth performance and nutrient digestibility of the broilers selected. The separate treatments contained 10 replicates (15 birds per replicate). The treatments were composed of: 1) CON, basal diet; 2) T1, basal diet + 0.03% protease; 3) T2, basal diet + 0.06% protease; and 4) T3, basal diet + 0.09% protease. Next, the broiler chick sample tissue was harvested from the CON and T3 groups in order to conduct gene expression analysis following the feeding trials the broilers underwent. Our team discovered that the broilers fed protease diets possessed increased body weight and an average daily gain, but conversely, had lower feed conversion ratios when their dietary protease levels increased from 0% to 0.09% (p < 0.05). Additionally, significant linear improvements were identified among the nutrient digestibility of dry matter, crude protein, energy, and amino acids within broilers supplied with protease diets when contrasted and compared with broilers supplied with the basal diet (p < 0.05). In addition, the gene expression of the genes IGF1, IGF2, GH, and LEP in the liver, and the genes MYOD1 and MYOG in the breast muscles, was significantly increased after broilers were fed with a protease diet as compared to broilers that subsisted on a basal diet (p < 0.05). Protease supplementation also raised the expression levels within these amino acid transporters: SCL6A19, SLC7A1, SLC7A7, SLC7A2, SLC7A6, SLC7A9, and SLC15A1, located in the small intestine, when compared to the basal diet (p < 0.05). Our results suggest that protease supplementation in their diet improved the growth performance of broilers via an increase in the expression growth-related genes within broiler liver and muscle tissue. In addition, protease supplementation enhanced broiler digestibility via the upregulation of amino acid transporter expression within the small intestine.

Effects of Ursolic Acid on Intestinal Health and Gut Bacteria Antibiotic Resistance in Mice

“…Besides, we found that UA promoted the expression of amino acid transporters (B 0 AT1, ASCT2, EAAT2, EAAT3). It is certain that there is a positive correlation between the increased expression of amino acid transporter and the growth performance (Zhang Y. L. et al, 2019;Park et al, 2020). However, there were few reports on the effect of UA on amino acid transporters so far and the underlying mechanisms should be further explored.…”

Comparative effects of proteases on performance, carcass traits and gut structure of broilers fed diets reduced in protein and amino acids

“…The benefits of the dietary supplementation of protease on growth performance are ascribed to increased CP digestibility [45][46][47][48], and they may be attributed to changes in intestinal morphology [38,49], reductions in the impact of antinutrients including trypsin inhibitors [50][51][52], and shifts in the microbiome [20,21]. The specific mode of action of dietary protease supplementation on intestinal morphology has not been well understood.…”

Lysolecithin Improves Broiler Growth Performance through Upregulating Growth-Related Genes and Nutrient Transporter Genes Expression Independent of Experimental Diet Nutrition Level

“…The RNA extraction and qRT-PCR analysis were performed according to the method of Park et al [ 9 ]. The detailed operation steps were as follow.…”

“…Additionally, Myoblasts play a crucial role in skeletal muscle formation during broiler growth. Myogenic differentiation1 (MYOD1) and myogenin (MYOG) are important components of myogenic regulatory factors (MRFs) which dominate myoblast differentiation [ 9 ]. Saxena’s study showed that energy and protein significantly affect the expression of growth-related genes [ 10 ].…”