The Effect of Processing Technique on Fat Graft Survival

Cañizares, Orlando; Thomson, Jennifer E.; Allen, Robert J.; Davidson, Edward H.; Tutela, John Paul; Saadeh, Pierre B.; Warren, Stephen M.; Hazen, Alexes

doi:10.1097/prs.0000000000003812

Cited by 50 publications

(32 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…11 Historically, the Coleman technique consists of loading 10-mL syringes with lipoaspirate, centrifugation at 3,000 rpm for 3 minutes, draining the blood and tumescent fraction from the bottom layer, and decanting and wicking the oil with a cotton pad for 3 minutes. 12,13 Coleman was against fat washing and exposing fat to air. 13 The advantage of centrifugation is an increased concentration of progenitor cells in the processed fat.…”

Section: Fat Graft Processing Techniques Centrifugationmentioning

confidence: 99%

“…13 The advantage of centrifugation is an increased concentration of progenitor cells in the processed fat. 12 Allen et al showed that greater percentages of highest density fractions of lipoaspirate persist over time compared with the lowest density fractions. 14 These concentrated lipoaspirates are believed to result in increased fat survival and slower reabsorp-tion rates through a vasculogenic mechanism with increased progenitor cells and vasculogenic mediators.…”

Section: Fat Graft Processing Techniques Centrifugationmentioning

confidence: 99%

“…This method has been reported to take approximately 2 to 4 minutes, which is comparable to the time required for centrifugation. 12 Benefits of cotton gauze rolling include low cost and little trauma to the fat particles. Though previously cited as being comparable in efficiency with the time it takes to centrifuge fat, cotton gauze rolling would presumably be more time-consuming for larger quantities of fat.…”

Section: Cotton Gauze Rollingmentioning

confidence: 99%

See 2 more Smart Citations

Fat Processing Techniques

Xue

Narvaez

Chu

et al. 2020

Seminars in Plastic Surgery

View full text Add to dashboard Cite

Autologous fat grafting is an aesthetic and reconstructive procedure in which an individual's own fat is harvested and injected into the soft tissues to correct contour and other abnormalities. Fat graft is considered the ideal soft tissue filler for its biocompatibility, lack of immunogenicity, and availability. The entire procedure of harvesting, processing, and transfer of fat graft affects fat graft take and effectiveness of fat grafting. This article will focus on the most common methods of fat graft processing, including centrifugation, cotton gauze rolling, sedimentation, and filtration/washing. The fragility of the harvested adipocytes makes the technique of fat graft processing of utmost importance, as blood and other unnecessary cellular fragments are removed. Each fat graft processing method has its own merits and shortcomings; however, due to a lack of well-defined prospective studies, there is no evidence to support one processing method as superior to another.

show abstract

Section: Fat Graft Processing Techniques Centrifugationmentioning

confidence: 99%

Section: Fat Graft Processing Techniques Centrifugationmentioning

confidence: 99%

Section: Cotton Gauze Rollingmentioning

confidence: 99%

See 1 more Smart Citation

Fat Processing Techniques

Xue

Narvaez

Chu

et al. 2020

Seminars in Plastic Surgery

View full text Add to dashboard Cite

show abstract

“…Coleman technique remains the gold standard for liposuction and lipofilling, but several studies have investigated the possibility to modify centrifugation parameters, in particular, reducing speed, because even the centrifuging technique is not always considered a minimal tissue manipulation technique. In fact, an excessive magnitude of centrifugal force can cause the rupture of the superficial fragile adipocytes and a loss of viability of ASCs (Bellini et al, 2017;Canizares et al, 2017;Coleman & Katzel, 2015;Galiè, Pignatti, Scambi, Sbarbati, & Rigotti, 2008;Girard et al, 2015;Palumbo et al, 2015;Strong et al, 2015).…”

mentioning

confidence: 99%

“…Various processing techniques have been designed as an alternative to centrifugation (Brzezienski & Jarrell, 2016;Canizares et al, 2017;Cleveland et al, 2015;Gabriel et al, 2017;Gal et al, 2019;Hivernaud et al, 2015;Tremolada et al, 2016). Others have been conceptualized as complementary to a reduced speed centrifugation (Boureaux et al, 2016;Cleveland et al, 2015;De Francesco et al, 2018;El-Sabbagh, 2017;Girard et al, 2015;Hanson et al, 2019;Hivernaud et al, 2015;Osinga et al, 2015;Strong et al, 2015).…”

mentioning

confidence: 99%

Comparative technical analysis of lipoaspirate mechanical processing devices

Veronese

Prè

Conti

et al. 2020

J Tissue Eng Regen Med

View full text Add to dashboard Cite

Fat grafting is a well‐established procedure in reconstructive, aesthetic, and regenerative medicine, in particular due to the presence in the adipose tissue of a high concentration of mesenchymal stem cells. The need to reduce fat processing times, for an immediate clinical use and regulatory restrictions on the degree of manipulation of human tissues, has led to the development of numerous devices for the mechanical, nonenzymatic processing of adipose tissue. The aim of this study is to describe the state of the art of mechanical devices used for fat processing, performing a technical analysis of the currently commercially available devices. This should facilitate the development of new devices that improve therapeutic results.

show abstract

A Simple Apparatus for Testing and Washing Filter Candles

Krock¹

1923

JAMA

View full text Add to dashboard Cite

Background: Lipoaspirate contains noxious substances derived from liposuction. Therefore, extensive washing is recommended before the lipoaspirate is processed further for culture or fat grafting. Washing a small amount of lipoaspirate may not pose a problem, but washing a large volume of lipoaspirate may be cumbersome, time consuming, and requires a lot of phosphate buffered saline (PBS). Objective: To introduce a simple method for lipoaspirate washing using fine-mesh stainless-steel tea or coffee filter, a small tea spoon, and a porcelain bowl. Methods: The filter was used to collect the adipose tissue fragments. Further washing of the fragments was achieved by soaking the adipose tissue containing filter in a PBS containing porcelain bowl and stirring using a small tea spoon to transfer the contaminating materials to the PBS. Enzymatic processing to dissociate the cells from the tissue and primary cultures was conducted as usual in MesenCult. Results: Using the equipment mentioned above, the adipose tissue fragments were readily separated from the blood, free lipids, anesthetics, and other noxious material in the liquid portion. This simple method saves time and PBS compared with previously described methods. Further enzymatic processing produced sufficient cells to be cultured, and culture results showed plastic adherent cells on day 2 that became confluent on day 6. Conclusion: Lipoaspirate washing using a fine mesh stainless steel filter is time saving and produced cells that grow well in MesenCult.

show abstract

The Effect of Processing Technique on Fat Graft Survival

Abstract: Therapeutic, V.

Cited by 50 publications

References 31 publications

Fat Processing Techniques

Fat Processing Techniques

Comparative technical analysis of lipoaspirate mechanical processing devices

A Simple Apparatus for Testing and Washing Filter Candles

Contact Info

Product

Resources

About