Comparative technical analysis of lipoaspirate mechanical processing devices

Veronese, Sheila; Prè, Elena Dai; Conti, Giamaica; Busato, Alice; Mannucci, Silvia; Sbarbati, Andrea

doi:10.1002/term.3093

Cited by 5 publications

(3 citation statements)

References 86 publications

(136 reference statements)

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“…Other publications have reported clinical outcome data alone from using these POC devices/systems (Table 5), but only Lipogems [7, 21, 36, 47, 57–59, 72, 73, 76], MyStem EVO [55] and Hy‐Tissue SVF [71] been used in orthopaedic related studies. Lipogems is a closed system which performs washing, filtration and sedimentation, with manual shaking and emulsification also required [74]. It has become popular in orthopaedics, having established an early patent for clinical use [68], as well as being a user‐friendly system [68].…”

Section: Discussionmentioning

confidence: 99%

The composition of cell-based therapies obtained from point-of-care devices/systems which mechanically dissociate lipoaspirate: a scoping review of the literature

et al. 2022

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Purpose Cell-based therapies using lipoaspirate are gaining popularity in orthopaedics due to their hypothesised regenerative potential. Several ‘point-of-care’ lipoaspirate-processing devices/systems have become available to isolate cells for therapeutic use, with published evidence reporting their clinical relevance. However, few studies have analysed the composition of their ‘minimally-manipulated’ cellular products in parallel, information that is vital to understand the mechanisms by which these therapies may be efficacious. This scoping review aimed to identify devices/systems using mechanical-only processing of lipoaspirate, the constituents of their cell-based therapies and where available, clinical outcomes. Methods PRISMA extension for scoping reviews guidelines were followed. MEDLINE, Embase and PubMed databases were systematically searched to identify relevant articles until 21st April 2022. Information relating to cellular composition and clinical outcomes for devices/systems was extracted. Further information was also obtained by individually searching the devices/systems in the PubMed database, Google search engine and contacting manufacturers. Results 2895 studies were screened and a total of 15 articles (11 = Level 5 evidence) fulfilled the inclusion criteria. 13 unique devices/systems were identified from included studies. All the studies reported cell concentration (cell number regardless of phenotype per millilitre of lipoaspirate) for their devices/systems (range 0.005–21 × 106). Ten reported cell viability (the measure of live cells- range 60–98%), 11 performed immuno-phenotypic analysis of the cell-subtypes and four investigated clinical outcomes of their cellular products. Only two studies reported all four of these parameters. Conclusion When focussing on cell concentration, cell viability and MSC immuno-phenotypic analysis alone, the most effective manual devices/systems were ones using filtration and cutting/mincing. However, it was unclear whether high performance in these categories would translate to improved clinical outcomes. Due to the lack of standardisation and heterogeneity of the data, it was also not possible to draw any reliable conclusions and determine the role of these devices/systems in clinical practice at present. Level of Evidence Level V Therapeutic.

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Section: Discussionmentioning

confidence: 99%

The composition of cell-based therapies obtained from point-of-care devices/systems which mechanically dissociate lipoaspirate: a scoping review of the literature

et al. 2022

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“…These methods utilize physical forces to gently break down the extracellular matrix and isolate stem cell-rich products through various techniques, including centrifugation, filtration, decantation, emulsification, sedimentation, squeezing, or washing. 55,56 While this can be done manually, mechanical devices developed for intraoperative use provide a more controlled approach, reducing time constraints and potential contamination risks. [57][58][59][60][61] Devices differ based on their specific features, the combination of isolation techniques used, and the degree of tissue dissociation and final product.…”

Section: Isolation Methodsmentioning

confidence: 99%

Autologous Fat Transfer in Craniofacial Surgery

Malisetyan,

Harrison,

Shahriari

et al. 2024

FACE

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Over the past two decades, autologous fat transfer has garnered significant recognition and widespread adoption within esthetic and reconstructive surgical domains. In craniofacial surgery, fat transplantation is frequently employed to address soft tissue volumetric deficiencies and asymmetries that influence facial contours. While adipose tissue (AT) is widely regarded as an optimal choice for augmentation due to its abundant availability and biocompatibility, the unpredictability and heightened resorption rates observed with traditional lipofilling techniques present a challenge for clinicians. Adipose-derived stem cells (ASCs) housed within the grafted tissue play a pivotal role in graft survival and offer avenues for tissue repair due to their angiogenic, anti-inflammatory, and immunosuppressive properties. Micro Fragmentation of Adipose Tissue (MFAT), utilized in several FDA-approved processing devices, has demonstrated promising outcomes in treating osteoarthritic joints, with success primarily attributed to enhanced paracrine function of ASCs via preservation of the perivascular niche. Currently, its application for treating bone or articular defects in the craniofacial region, including abnormalities of the temporomandibular joint, remains limited. This scarcity underscores the need for further investigation prior to its widespread integration into clinical practice.

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“…Fat grafting is a consolidated procedure in reconstructive, aesthetic, and regenerative medicine due to the presence in the adipose tissue of a high concentration of MSCs [ 68 , 69 ]. The regenerative potential of adipose tissue is well known, and it is continuously updated thanks to numerous studies concerning the application of autologous adipose tissue transplantation in reconstructive and regenerative medicine [ 70 , 71 , 72 ].…”

Section: Fat As An Optimal Source Of Muse Cells Isolationmentioning

confidence: 99%

Multilineage-Differentiating Stress-Enduring Cells (Muse Cells): An Easily Accessible, Pluripotent Stem Cell Niche with Unique and Powerful Properties for Multiple Regenerative Medicine Applications

et al. 2023

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Cell-based therapy in regenerative medicine is a powerful tool that can be used both to restore various cells lost in a wide range of human disorders and in renewal processes. Stem cells show promise for universal use in clinical medicine, potentially enabling the regeneration of numerous organs and tissues in the human body. This is possible due to their self-renewal, mature cell differentiation, and factors release. To date, pluripotent stem cells seem to be the most promising. Recently, a novel stem cell niche, called multilineage-differentiating stress-enduring (Muse) cells, is emerging. These cells are of particular interest because they are pluripotent and are found in adult human mesenchymal tissues. Thanks to this, they can produce cells representative of all three germ layers. Furthermore, they can be easily harvested from fat and isolated from the mesenchymal stem cells. This makes them very promising, allowing autologous treatments and avoiding the problems of rejection typical of transplants. Muse cells have recently been employed, with encouraging results, in numerous preclinical studies performed to test their efficacy in the treatment of various pathologies. This review aimed to (1) highlight the specific potential of Muse cells and provide a better understanding of this niche and (2) originate the first organized review of already tested applications of Muse cells in regenerative medicine. The obtained results could be useful to extend the possible therapeutic applications of disease healing.

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Comparative technical analysis of lipoaspirate mechanical processing devices

Cited by 5 publications

References 86 publications

The composition of cell-based therapies obtained from point-of-care devices/systems which mechanically dissociate lipoaspirate: a scoping review of the literature

The composition of cell-based therapies obtained from point-of-care devices/systems which mechanically dissociate lipoaspirate: a scoping review of the literature

Autologous Fat Transfer in Craniofacial Surgery

Multilineage-Differentiating Stress-Enduring Cells (Muse Cells): An Easily Accessible, Pluripotent Stem Cell Niche with Unique and Powerful Properties for Multiple Regenerative Medicine Applications

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