Aim: The aim of this study was to assess the influence of Proanthocyanidin (PA) application on bond strength of glass fiber posts to root canal dentin using self-etch adhesive luting system. Materials and Methods: Twenty single rooted mandibular premolars, freshly extracted for orthodontic purpose, were decoronated below the cementoenamel junction, and standardized to 14mm in length. Post space preparation was done upto No 3 Peeso reamer during which canals were irrigated with conventional irrigation protocol. Teeth were randomly divided into two groups (n=20 each) Group 1: Pre-treatment with PA and Group 2 served as the control group. Both Groups were restored with fiber posts bonded with RELYX U200 self-etch adhesive system. Teeth were then stored in distilled water for three months at 37° C. One mm slices were obtained and push-out bond strength testing was performed. Failure patterns were assessed under stereomicroscope at 20x magnification. Results: Individual bond strength values were calculated and statistically analysed with the chi-square test and independent-ttest. A statistically significant difference existed between the two Groups (p <0.001) with Group 1 showing a mean bond strength value greater than Group 2. Conclusion: Pretreatment with PA preserves the bond strength of fiber posts to root dentin bonded with a self-etch adhesive system. The results indicate that PA can be used as a biocompatible pretreatment alternative to improve bond strength stability of dentin-adhesive interfaces in root canals.