The effect of pre-existing bacteriuria on bladder resistance to superinfection in the rabbit

Pm, Hanno; Fritz, Robert; Aj, Wein; Sg, Mulholland

doi:10.1007/bf00256683

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…Other studies performed in our laboratory using the [13]. We have shown that the protective activity of the nor mal sialomucin layer can be duplicated by the instillation of small amounts of an exogenous mucopolysaccharide, the glycosaminoglycan heparin, in a bladder denuded of its natural protective mucous [14], This finding tends to substantiate the thesis that it is indeed the mucopolysac charide coating of the bladder that acts as an 'antiadher ence factor'.…”

Section: Monis and Zambrano [2] And Monis And Dorfmansupporting

confidence: 72%

Heparin - an Examination of Its Antibacterial Adsorption Properties

Hanno¹,

Fritz²,

Mulholland³

et al. 1981

Eur Urol

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One of the primary antibacterial defense mechanisms of the bladder is the action of the luminal mucopolysaccharide layer against adsorption of innoculated bacteria. Previous studies have shown that local instillation of the mucopolysaccharide heparin can prevent bacterial adsorption on the bladder mucosa denuded of this ‘antiadherence factor’. To determine whether this action was due to the mucopolysaccharide composition of heparin, or rather to its anticoagulant property, protamine sulfate (a basic protein with anticoagulant properties) was tested for antiadsorptive efficacy. Protamine offered no protection against bacterial adherence in the rabbit model. It appears that heparin’s protective effect is unrelated to its action as an anticoagulant.

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Section: Monis and Zambrano [2] And Monis And Dorfmansupporting

confidence: 72%

Heparin - an Examination of Its Antibacterial Adsorption Properties

Hanno¹,

Fritz²,

Mulholland³

et al. 1981

Eur Urol

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The effects of heparin on the adherence of five species of urinary tract pathogens to urinary bladder mucosa

1984

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Previous studies performed in our laboratory have indicated that the primary antibacterial defense mechanism of the rabbit urinary bladder is the antiadsorptive action of the surface mucopolysaccharide. Removal of this layer with an acid rinse increases bacterial adherence up to 100 fold. Exogenous mucopolysaccharide (heparin) has been shown to restore Escherichia coli adherence to control levels. To determine whether this antiadherence action of heparin is species specific, we compared the adherence of 5 common urinary tract pathogens (Escherichia coli, Klebsiella ozonae, Proteus mirabilis, Pseudomonas aeruginosa, and Streptococcus fecalis) to both mucin intact and mucin deficient rabbit bladders with and without prior heparin exposure. Bacteria were radiolabeled by addition of 3H-adenine to the culture broth so that the number of bacteria adhering to the bladder could be determined using liquid scintillation spectrophotometry. Results were as follows: Acid removal of the mucin layer significantly increased the adherence approximately 10 fold for all 5 species tested. Briefly exposing the mucin deficient bladders to heparin decreased the adherence of all species tested except Pseudomonas to mucin intact control levels. Heparin treatment of mucin intact bladders slightly decreased adherence of all species except Pseudomonas below mucin intact controls, however, results were not statistically significant. The magnitude of Klebsiella adherence was nearly 20 fold greater than all other species tested. While this non-species specific adherence inhibition of heparin may prove useful in the clinical setting, it appears to be less effective against Pseudomonas.

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