Heparin - an Examination of Its Antibacterial Adsorption Properties

Hanno, Philip M.; Fritz, Robert; Mulholland, S. Grant; Wein, A.J.

doi:10.1159/000473265

Cited by 5 publications

(3 citation statements)

References 12 publications

(13 reference statements)

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“…However, the mechanism by which heparin inhibits bacterial adhesion remains unclear, although it is not related to its action as an anticoagulant. 23 Heparin is composed of long hydrophilic chains and has a negative charge, and these factors are likely to play a role in inhibiting adherence. 24 S. epidermidis also has a negative charge, which may lead to the bacterium being repelled from the heparin preconditioned surface.…”

Section: Discussionmentioning

confidence: 99%

Reduction of Staphylococcus epidermidis adhesion to indwelling medical devices: a simple procedure

Sousa

Henriques

Teixeira

et al. 2008

British Journal of Biomedical Science

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The present study aims to find a method to reduce Staphylococcus epidermidis adhesion to acrylic and silicone--two materials used commonly in medical devices--by heparin and gentian violet surface preconditioning. Different periods of heparin preconditioning are studied to evaluate the influence of preincubation time on the reduction of bacterial adhesion. A two-hour period was chosen and applied in the adhesion assays with either heparin or gentian violet. Squares of the materials with adherent cells were also analysed by scanning electron microscopy (SEM). Results of adhesion assays showed a significant reduction (53-90%, P<0.05) in bacterial adhesion to silicone and acrylic after precontact with the conditioning substances. No statistical differences (P>0.05) were found between the extent of adhesion on silicone coupons precontacted either with heparin or gentian violet for each of the strains tested. On acrylic, heparin was more efficient (P<0.001) in reducing S. epidermidis IE186 adhesion than was gentian violet (85% and 53% reductions, respectively). Therefore, immersion of acrylic and silicone in heparin or gentian violet may constitute a simple and effective method by which to reduce S. epidermidis adhesion to medical devices.

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Section: Discussionmentioning

confidence: 99%

Reduction of Staphylococcus epidermidis adhesion to indwelling medical devices: a simple procedure

Sousa

Henriques

Teixeira

et al. 2008

British Journal of Biomedical Science

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“…The function of the GAG layer is proposed to be an antibacterial coating of the bladder to prevent bacterial (specifically, bacterial pili) adherence . Removal of the GAG layer has been shown to increase bacterial adherence 100‐fold . Subsequent research has shown that a deficiency in the GAG layer increases the rate of UTI .…”

Section: Glycosaminoglycansmentioning

confidence: 99%

Recurrent urinary tract infections: a critical review of the currently available treatment options

Negus

Phillips

Hindley

2020

The Obstetric & Gynaecologis

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Urinary tract infections (UTIs) are the most common infections in women worldwide. Prevalence increases with age to 20% over the age of 60. Recurrence is common. UTI is a clinical and socio-economic burden and a cause of significant psychological distress to the patient. Acute UTI needs swift assessment, diagnosis and treatment to prevent ascending or systemic infection. Antibiotics remain the mainstay of treatment in acute UTI; it is thought that up to 50% of antibiotic prescriptions are inappropriate or unnecessary and may increase the risk of bacterial resistance.Management strategies to prevent recurrent UTI are heterogeneous. Learning objectivesTo understand the rationale of why preventing recurrent UTI (rUTI) is important. To develop an awareness of the non-physical effects of rUTI. To understand the critical appraisal of the current and developing treatments for rUTI.

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“…Indeed, the GAG layer might play a greater role preventing bacterial adherence, as described by Hanno et al (1981) (38). The GAG layer might also be important at preventing the formation and attachment of particulates to the urothelium, and thereby, subsequent stone formation (39).…”

Section: The Blood-urine Barriermentioning

confidence: 99%

Early experimental results of using a novel delivery carrier, hyaluronan-phosphatidylethanolamine (HA-PE), which may allow simple bladder instillation of botulinum toxin A as effectively as direct detrusor muscle injection

Shatoury

DeYoung

Turley

et al. 2018

Journal of Pediatric Urology

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Early experimental results of using a novel delivery carrier, Hyaluronan-phosphatidylethanolamine (HA-PE) which may allow simple bladder instillation of Botulinum Toxin A as effectively as direct detrusor muscle injection. Abstract Intra-detrusor Botulinum Toxin-A (BTX-A) injection is the gold standard treatment for detrusor overactivity in clinical practice when medical treatment fails. Despite satisfactory results of BTX-A injection, the endoscopic procedure still has risks of anaesthetic and operative complications. Several studies have investigated more simple ways to deliver BTX-A to the detrusor muscle but with limited success. In our study, we assessed the evidence of BTX-A delivery using a newly modulated hyaluronan-phosphatidylethanolamine (HA-PE) carrier in a rat model through BTX-A-HA-PE bladder instillations. We used histological evidence of SNAP-25 protein cleavage and detection of Alexa Fluor®594-labelled BTX-A as a proof of physical BTX-A delivery across the urothelium. In addition, we assessed the functional impact of using HA-PE on rat detrusor muscle through urodynamic assessment both at baseline and 2 weeks later after 1% acetic acid instillation. We found positive histological evidence of SNAP-25 cleavage, detection of Alexa Fluor®594-BTX-A red fluorescence in bladder tissue supporting that HA-PE may be an efficient carrier to deliver BTX-A across the urothelium. Mohamed G. El Shatoury

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Heparin - an Examination of Its Antibacterial Adsorption Properties

Cited by 5 publications

References 12 publications

Reduction of Staphylococcus epidermidis adhesion to indwelling medical devices: a simple procedure

Reduction of Staphylococcus epidermidis adhesion to indwelling medical devices: a simple procedure

Recurrent urinary tract infections: a critical review of the currently available treatment options

Early experimental results of using a novel delivery carrier, hyaluronan-phosphatidylethanolamine (HA-PE), which may allow simple bladder instillation of botulinum toxin A as effectively as direct detrusor muscle injection

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