The Effect of Post warming Culture Period Between Thawing and Transfer of Cryopreserved Embryos on Reproductive Outcomes After In Vitro Fertilization (IVF): A Systematic Review and Meta-analysis

Sordia-Hernández, Luis H.; Martinez, Felipe A. Morales; Orozco, Eduardo Gutiérrez; Flores-Rodríguez, Andrea; Leyva-Camacho, Paloma C.; Alvarez‐Villalobos, Neri Alejandro; Zuñiga-Hernandez, Jorge A

doi:10.18502/jri.v22i2.5792

Cited by 4 publications

(8 citation statements)

References 18 publications

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“…Our data also clearly showed that the live birth rate after transfer of thawed blastocysts was not affected by the post‐warming culture time. These findings are similar to those of a meta‐analysis including five studies that investigated the effect of post‐warming culture on reproductive outcomes 23 . The meta‐analysis found no differences in clinical pregnancy, implantation, and live birth rates between culture times of 2 h vs. overnight 23 .…”

Section: Discussionsupporting

confidence: 83%

“…These findings are similar to those of a meta-analysis including five studies that investigated the effect of post-warming culture on reproductive outcomes. 23 The meta-analysis found no differences in clinical pregnancy, implantation, and live birth rates between culture times of 2 h vs. overnight. 23 Haas et al (2018) investigated the delay of blastocyst transfer until 20-22 h after warming, and reported that the ongoing pregnancy rate was the same after transfer of goodquality blastocysts, whether they developed further during the culture or not.…”

Section: Discussionmentioning

confidence: 92%

“…23 The meta-analysis found no differences in clinical pregnancy, implantation, and live birth rates between culture times of 2 h vs. overnight. 23 Haas et al (2018) investigated the delay of blastocyst transfer until 20-22 h after warming, and reported that the ongoing pregnancy rate was the same after transfer of goodquality blastocysts, whether they developed further during the culture or not. 24 In our study, when there was at least one good-quality blastocyst, extending the post-warming culture period to more than Abbreviations: CI, confidence interval; PCOS, polycystic ovary syndrome.…”

Section: Discussionmentioning

confidence: 92%

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Effect of post‐warming culture time on the live birth rate after frozen embryo transfer

Pham

Nguyễn

et al. 2022

Reprod Medicine & Biology

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After the first report of a live birth achieved using transfer of frozen embryos 1 and the success of vitrification, 2 embryo cryopreservation is widely used at in vitro fertilization (IVF) centers. This has resulted in a dramatic increase in the number of pregnancies conceived after frozen embryo transfer (FET). 3 FET avoids the detrimental effects of high-dose hormones used for controlled ovarian stimulation on the endometrium and prevents ovarian hyperstimulation syndrome (OHSS). 4 A number of studies have suggested that fertility outcomes are comparable after FET compared with fresh embryo transfer. [5][6][7] As a result, embryo cryopreservation has become a fundamental part of assisted reproduction technology (ART).After thawing, embryos can be cultured in vitro until FET. The purpose of this culture is to help the embryo recover, and stabilize the osmotic pressure after freezing and thawing. Although the

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 92%

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Effect of post‐warming culture time on the live birth rate after frozen embryo transfer

Pham

Nguyễn

et al. 2022

Reprod Medicine & Biology

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“…However, it was comparable between cycles that had and had not resulted in a live birth. Moreover, the time interval between embryo warming and transfer was found not to affect reproductive outcome [22]. Third, there was a significant heterogeneity in the characteristics of the studied cycles, including the use of PGT-A in some, but not all, cycles.…”

Section: Discussionmentioning

confidence: 91%

Post-warming embryo morphology is associated with live birth: a cohort study of single vitrified-warmed blastocyst transfer cycles

Allen

Hale

Lantsberg

et al. 2022

J Assist Reprod Genet

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Purpose This study aims to examine whether blastocyst morphology post-warming correlates with live birth. Methods In this cohort study, morphological characteristics post-warming were reviewed in all single vitrified-warmed blastocyst transfer cycles performed between November 2016 and May 2017. Immediately before transfer, the degree of blastocoel re-expansion was graded as A, fully expanded; B, partially expanded ≥ 50%; C, partially expanded < 50%; and D, collapsed. The degree of post-warming cell survival was graded on a scale of 50 to 100% and was then classified into 4 groups: very low 50–70%, low 71–80%, moderate 81–90%, and high 91–100%. Results Overall, 612 cycles were reviewed, of which 196 included PGT-A tested embryos. The live birth rate (LBR) increased from 11.4% in the collapsed blastocysts group to 38.9% in the post-warming full re-expansion group (p < 0.001) and from 6.5% for blastocysts with a very low cell survival rate to 34.7% for blastocysts with high cell survival rate (p = 0.001). LBR was 6.7% for blastocysts with the worst post-warming morphological characteristics, namely, collapsed with very low cell survival rate. On multivariate analyses, partial blastocyst re-expansion ≥ 50%, full re-expansion, and high cell survival rate remained significantly associated with live birth, after controlling for female age, pre-vitrification morphological grading, and PGT-A. A sub-analysis of cycles using PGT-A tested embryos showed similar results. Conclusion Post-warming re-expansion and high cell survival rate are associated with higher LBR in euploid and untested blastocysts. However, embryos with poor post-warming morphology still demonstrate a considerable probability of live birth, and they should not be discarded.

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“…There is no final consensus on the optimum thaw culture time required. [ 5 ] This study was done to find the best day of thawing, culture and day of transfer of embryos by comparing their clinical pregnancies to improve our clinical outcome.…”

Section: Introductionmentioning

confidence: 99%

Impact of post-thaw incubation time of frozen embryos on clinical pregnancy rate

et al. 2023

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A BSTRACT Background: Vitrification of embryos following a single-controlled ovarian stimulation has been the strategy practised now in many in vitro fertilisation clinics to minimise the risk of early ovarian hyper stimulation syndrome, to reduce multiple pregnancy rates and to improve cumulative pregnancy rates. In recent years, advances in vitrification techniques and improved culture conditions have led to good post-thaw embryo survival rates, thereby increasing pregnancy rates of frozen embryo transfer (FET) cycles. Aim: The aim of this study was to analyse the effect of post-thaw incubation time of frozen embryos on the clinical pregnancy rates (CPRs) of frozen embryo transfer (FET) cycles. Settings and Design: This was a retrospective, comparative study done at a teaching hospital in assisted reproductive treatment. Materials and Methods: Three hundred and ten FET cycles were analysed, of which 125 had day 2 freezing and 185 had day 3 freezing. Depending upon the day of thawing and day of transfer, FET cycles were divided into six groups: Group 1 (day 2 thawing and day 3 transfer), Group 2 (day 2 thawing and day 4 transfer), Group 3 (day 2 thawing and day 5 transfer), Group 4 (day 3 thawing and day 3 transfer), Group 5 (day 3 thawing and day 4 transfer) and Group 6 (day 3 thawing and day 5 transfer). Statistical Analysis Used: Statistical analysis was performed using version 14 R software version 4.0.1 (2020-06-06) (R foundation for Statistical Computing, Vienna, Austria). A P < 0.05 is taken as significant. Results: The CPR of Group 4 was 42.4% which was more than that of the other groups but it did not reach statistical significance. Conclusions: Short incubation time of 2–4 h is as effective as an extended incubation time in terms of CPRs of FET cycles.

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The Effect of Post warming Culture Period Between Thawing and Transfer of Cryopreserved Embryos on Reproductive Outcomes After In Vitro Fertilization (IVF): A Systematic Review and Meta-analysis

Cited by 4 publications

References 18 publications

Effect of post‐warming culture time on the live birth rate after frozen embryo transfer

Effect of post‐warming culture time on the live birth rate after frozen embryo transfer

Post-warming embryo morphology is associated with live birth: a cohort study of single vitrified-warmed blastocyst transfer cycles

Impact of post-thaw incubation time of frozen embryos on clinical pregnancy rate

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