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After the first report of a live birth achieved using transfer of frozen embryos 1 and the success of vitrification, 2 embryo cryopreservation is widely used at in vitro fertilization (IVF) centers. This has resulted in a dramatic increase in the number of pregnancies conceived after frozen embryo transfer (FET). 3 FET avoids the detrimental effects of high-dose hormones used for controlled ovarian stimulation on the endometrium and prevents ovarian hyperstimulation syndrome (OHSS). 4 A number of studies have suggested that fertility outcomes are comparable after FET compared with fresh embryo transfer. [5][6][7] As a result, embryo cryopreservation has become a fundamental part of assisted reproduction technology (ART).After thawing, embryos can be cultured in vitro until FET. The purpose of this culture is to help the embryo recover, and stabilize the osmotic pressure after freezing and thawing. Although the

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