The effect of Portulaca oleracea and α-linolenic acid on oxidant/antioxidant biomarkers of human peripheral blood mononuclear cells

Abstract

OBJECTIVES:Various pharmacological effects including antioxidant property of Portulaca oleracea L. were reported previously. In the present study, the effect of the extract of the plant and its constituent, α-linolenic acid (ALA), on oxidant and antioxidant markers of PHA/non-stimulated human mononuclear cells was evaluated.MATERIALS AND METHODS:The effect of 10, 40, and 160 μg/ml of P. oleracea and 5, 15, and 45 μg/ml of ALA or dexamethasone (0.1 mM) on nitric oxide (NO), malondialdehyde (MDA), total thiol (SH), superoxide dismutase (SOD), and catalase (CAT) in the supernatant of phytohemagglutinin-A (PHA)- and nonstimulated lymphocytes was examined (n = 6 for each group).RESULTS:In nonstimulated cells, dexamethasone, high concentration of the extract (160 μg/ml), and ALA (45 μg/ml) significantly increased thiol, CAT, and SOD values. Dexamethasone and high concentration of ALA significantly reduced MDA value (P < 0.01 to P < 0.001). However, the levels of NO and MDA due to dexamethasone and 160 μg/ml of the extract and 15 and 45 μg/ml of ALA treatment were also reduced in PHA-stimulated cells (P < 0.001 for all cases). Treatment of stimulated lymphocyte by dexamethasone and two higher concentrations of the extract and ALA also leads to increased levels of thiol, CAT, and SOD (P < 0.05 to P < 0.001).CONCLUSIONS:P. oleracea and ALA, as well as dexamethasone, decreased NO and MDA levels but increased antioxidant agents in human lymphocytes. These results suggest that P. oleracea and ALA may have therapeutic effect in diseases associated with enhancement of oxidation agents as an antioxidant agent.

Protective Effect of Portulaca oleracea on Streptozotocin-Induced Type I Diabetes-Associated Reproductive System Dysfunction and Inflammation

“…Moreover, PO extract notably ameliorated the oxidative stress induced by streptozotocin in the testicular tissue of rats. This is in line with previous works that demonstrated the anti-oxidative properties of PO in both male [55,56] and female rodents [57,58].…”

A review on bioactive phytochemicals and ethnopharmacological potential of purslane (Portulaca oleracea L.)

“…Antioxidants are very important for human health as they reduce risk of cell damage by free radicals [ 7 ]. Several studies have investigated and proved the antioxidant potential of purslane [ 27 , 30 , 142 , 176 ]. Several compounds have been isolated from purslane and their antioxidant activities have been proved.…”

“…Recent advancements in the quantitative tools for the analysis of phytochemicals has led to the identification of several hundred metabolites from various parts of the purslane [ 5 , 7 , 10 , 18 , 21 , 22 ]. Using ethnobotanical leads, scientists have tested the efficacy of purslane as a medicinal plant using in vitro as well as in vivo studies and have found impressive results on its pharmacological potential [ 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ]. The confirmation of the medicinal uses against the diseases using modern scientific studies provide evidence in support of the ethno medicinal properties of the purslane.…”

A review on bioactive phytochemicals, ethnomedicinal and pharmacological importance of Purslane (Portulaca oleracea L.)

“…Antioxidants are very important for human health as they reduce risk of cell damage by free radicals (Korade and Deokule, 2015). Several studies have investigated and proved ant-oxidant potential of Purslane (Alam et al, 2014;Baradaran-Rahimi et al, 2019;Yahyazadeh Mashhadi et al, 2018). Several compounds have been isolated from Purslane and their antioxidant activities have been proved.…”

“…Recent advancements in the quantitative tools for the analysis of phytochemicals has led to the identi cation of several hundred metabolites from various parts of the Purslane (Mohamed and Hussein, 1994;Uddin et al, 2014;Okafor and Ezejindu, 2014;Negi, 2018). Using ethnobotanical leads, scientists have tested the e cacy of Purslane as medicinal plant using in vitro as well as in vivo studies and found impressive results (Huang and Dong, 2011;Wang and Yang, 2010;Al-Sheddi et al, 2015;Bai et al, 2016;Yahyazadeh Mashhadi et al, 2018;Baradaran-Rahimi et al, 2019;Roozi, et al, 2019). The con rmation of the medicinal uses against the diseases using modern scienti c studies provide evidence in support of the ethno medicinal properties of the Purslane.…”