Protective Effect of Portulaca oleracea on Streptozotocin-Induced Type I Diabetes-Associated Reproductive System Dysfunction and Inflammation

Abstract

Background: Type-one diabetes (T1D), a chronic autoimmune disease with marked inflammatory responses, is associated with infertility complications and implications. Based on the anti-diabetic, antioxidant, and anti-hyperlipidemic potential of Portulaca oleracea (PO), this study aimed to evaluate the protective effect of this plant extract on streptozotocin-induced type-I-diabetes-associated reproductive system dysfunction and inflammation. Methods: Male rats were randomly divided into four experimental groups: control, diabetic, and treatment/s (PO extract at 100 or 300 mg/kg/daily). Then food and water consumption, body, testis and epididymis weights, histopathological evaluation, seminiferous tubules diameter, sperm count and motility, glucose levels, sex hormones, and inflammatory and oxidative stress markers were evaluated. Results: Our results showed that streptozotocin-induced diabetes significantly increased food and water consumption; increased glucose, MDA, TGF-β1, and TNF-α levels; and decreased the seminiferous tubules diameter, sperm count and motility, levels of LH, testosterone, total thiol, VEGF, and SOD activity. Interestingly, PO extract (phytochemically characterized by using liquid chromatography–mass spectrometry to detect bioactive molecules) significantly ameliorated these parameters and histopathological indexes’ damage in rats. Conclusion. Even if more preclinical assessments are needed to better characterize the mechanism/s of action, the results of this study will pave the way for the rational use of PO on diabetic-associated clinical complications and implications.

Is losartan a promising agent for the treatment of type 1 diabetes-induced testicular germ cell apoptosis in rats?

“…DM is a metabolic disease that poses a serious risk to public health and causes severe damage to a number of organs. This disease also regards as one of the important cause of morbidity and mortality worldwide Moreover, growing evidence suggests that DM is closely associated with fertility problems, particularly in the male [29]. In fact, previous clinical and pre-clinical studies have reported that DM causes serious disruptions in male reproductive function by paving the way for problems such as testicular tissue defects [29,30].…”

“…This disease also regards as one of the important cause of morbidity and mortality worldwide Moreover, growing evidence suggests that DM is closely associated with fertility problems, particularly in the male [29]. In fact, previous clinical and pre-clinical studies have reported that DM causes serious disruptions in male reproductive function by paving the way for problems such as testicular tissue defects [29,30]. Similar to the literature, the results of our histopathological examination (JTBS) revealed that DM was dramatically disrupted histo-architecture of the testicular tissue and caused histopathological alterations such as seminiferous tubular atrophy, tubular degeneration, interstitial edema and Leydig cells losses.…”

Curcumin‐based nanoformulations alleviate wounds and related disorders: A comprehensive review

“…Diabetes with induction in the secretion of inflammatory mediators by macrophage cells results in prolongation and an increase in inflammation, thereby delaying wound healing. [114][115][116][117][118][119][120][121] Therefore, the researchers fabricated CUR-loaded CTS NPs (CUR-CS-NPs) to evaluate their capability in a decrement in macrophage cellmediated inflammation and accelerating wound healing in a STZ-induced diabetic rat model. The findings revealed that CUR-CS-NPs with sustained drug release and better cell uptake, through the reduction in inflammation of macrophages and increment in angiogenesis, effectively accelerated the process of wound healing in comparison to the control group.…”

“…Diabetes with induction in the secretion of inflammatory mediators by macrophage cells results in prolongation and an increase in inflammation, thereby delaying wound healing 114–121 . Therefore, the researchers fabricated CUR‐loaded CTS NPs (CUR‐CS‐NPs) to evaluate their capability in a decrement in macrophage cell‐mediated inflammation and accelerating wound healing in a STZ‐induced diabetic rat model.…”

Anti-diabetic Effects of Two Species of Eryngium on Streptozotocin-induced Diabetic Mice

“…The mice were twelve weeks old with a weight range of 30 g. They were kept under standard conditions, including 12h light-dark, room temperature (20°C) with 60 ± 10% humidity, and were provided with suitable nutrition until the initiation of the tests. Then they were randomly divided into eight groups (n = 6), including group 1: Control group that received only water and food and experienced no intervention; group 2: Diabetic control group that received only STZ without medication; group 3: Diabetic group in which a low dose of extracts (100 mg/kg) was injected intraperitoneally; group 4: Diabetic group in which a high dose of the extracts (300 mg/kg) was injected intraperitoneally (17); group 5: Diabetic group that received a low dose of extracts (100 mg/kg) by gavage (oral); group 6: Diabetic group in which 300 mg/kg of extracts was gavaged; group 7: Control group in which 300 mg/kg of extracts was gavaged; and group 8: The group that received STZ and gavaged with 300 mg/kg of extracts simultaneously (for five days) (17)(18)(19).…”