The effect of pore size of silica gel and concentration of buffer on capillary chromatographic and capillary electrochromatographic enantioseparations using cellulose tris(3,5-dichlorophenylcarbamate)

Chankvetadze, Bezhan; Kartozia, Irma; Okamoto, Yoshio; Blaschke, Gottfried

doi:10.1002/1615-9314(20010801)24:8<635::aid-jssc635>3.0.co;2-q

Cited by 43 publications

(22 citation statements)

References 21 publications

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“…The effect of the amount of a chiral selector loaded onto silica gel is very significant for the enantioseparation characteristics as shown in several previous studies [31][32][33][34].…”

Section: Effect Of a Loading Of Chiral Selector On Clc And Cec Enantimentioning

confidence: 82%

“…Currently, the application of cellulose tris(3,5-dimethylphenylcarbamate (CDMPC) [9][10][11][12][27][28][29], cellulose tris(4-methylbenzoate) [27,30], cellulose tris(3,5-dichlorophenylcarbamate) (CDCPC) [31][32][33][34], amylose tris(3,5-dimethylphenylcarbamate) (ADMPC) [11,28,30] and amylose tris((S)-1-ethylphenylcarbamate) [1] have been reported for CEC enantioseparations in the wall-coated open-tubular [27] and packed capillaries [1-5, 9-12, 28-34]. Among the aforementioned polysaccharide derivatives, CDMPC [9,10,12,[27][28][29] and CDCPC [31][32][33][34] were studied in more detail while just a few separation examples were reported with other materials. CDMPC has been extensively investigated in combination with aqueous-organic mobile phases [9-12, 27, 29] while only few CEC enantioseparations with this material in nonaqueous alcohols have been reported [11,28,30].…”

Section: Introductionmentioning

confidence: 99%

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Enantioseparations in nonaqueous capillary liquid chromatography and capillary electrochromatography using cellulose tris(3,5-dimethylphenylcarbamate) as chiral stationary phase

et al. 2002

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The potential of the widely used chiral stationary phase for high-performance liquid chromatography (HPLC) enantioseparations, cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC, sold under the trade name Chiralcel OD) was evaluated under the conditions of nonaqueous capillary electrochromatography (CEC). The effect of the particle size of the silica gel, the loading of CDMPC on the silica gel and nature of the organic solvent, as well as electrolyte salts on the separation characteristics were investigated. This study illustrates the applicability of CDMPC for obtaining highly efficient enantioseparations under the conditions of nonaqueous CEC. Comparative study of enantioseparations in capillary liquid chromatography (CLC) and CEC indicated the significant advantages of CEC such as higher plate number at the similar linear flow rates of the mobile phase as well as better tolerance of higher linear flow rates.

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“…The effect of the amount of a chiral selector loaded onto silica gel is very significant for the enantioseparation characteristics as shown in several previous studies [31][32][33][34].…”

Section: Effect Of a Loading Of Chiral Selector On Clc And Cec Enantimentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Enantioseparations in nonaqueous capillary liquid chromatography and capillary electrochromatography using cellulose tris(3,5-dimethylphenylcarbamate) as chiral stationary phase

et al. 2002

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“…The previous reports of CEC enantioseparations on this kind of coated CSPs were performed under the mobile phases of acetonitrile-water or pure methanol and ethanol systems [9][10][11][12][13][14][15][16][17]. However, the typical mobile phase used for enantioseparations on polysaccharide derivative CSPs is the hexane-alcohol system according to the knowledge in HPLC [8].…”

Section: Separation Of Enantiomers With Nonaqueous Phasesmentioning

confidence: 99%

“…Recently, polysaccharide derivatives were also applied as CSPs for CEC separation of enantiomers based on the open tubular and packed capillary columns [9][10][11][12]. For instance, capillary columns packed with cellulose tri (3,5-dimethylphenylcarbamate) and cellulose tri(3,5-dichlorophenylcarbamate) coated CSPs have been used for resolution of enantiomers by Chankvetadze et al [13][14][15][16][17], and the effect of the pore size of silica gel and the mobile phase composition on enantioseparations was systematically investigated. The previous studies on CEC resolution of enantiomers were mainly concentrated on the coated type of polysaccharide derivative CSPs.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrochromatographic separation of enantiomers on chemically bonded type of cellulose derivative chiral stationary phases with a positively charged spacer

et al. 2003

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Capillary electrochromatographic separation of enantiomers on chemically bonded type of cellulose derivative chiral stationary phases with a positively charged spacer Positively charged chiral stationary phases (CSPs) were prepared for capillary electrochromatography (CEC) separation of enantiomers by chemically immobilizing cellulose derivatives onto diethylenetriaminopropylated silica (DEAPS) with tolylene-2,4-diisocyanate (TDI) as a spacer reagent. Anodic electroosmotic mobility was observed in both nonaqueous and aqueous mobile phases due to the positively charged amines on the surface of the prepared CSPs. For comparison, the traditionally used 3-aminopropyl silica (APS) was also adopted as the base material instead of DEAPS to prepare CSP. It was observed that the EOF on the DEAPS-based CSP was 18%-60% higher than that on the APS-based CSP under nonaqueous mobile phase conditions. Separation of enantiomers in CEC was performed on the positively charged CSPs with the nonaqueous mobile phases of pure ethanol or mixture of hexane-alcohol and the aqueous phases of acetonitrile-water or 95% ethanol. Fast separation of enantiomers was achieved on the newly prepared CSPs.

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“…Rapid chiral analyses with high efficiency and at low costs were achieved by CE mainly using the direct separation method where the chiral selector was simply added to the background electrolyte (BGE) (free zone electrophoresis, CZE) or bound to the capillary wall or to a stationary phase (capillary electrochromatography, CEC) [32][33][34][35][36].…”

Section: Introductionmentioning

confidence: 99%

Separation of reboxetine enantiomers by means of capillary electrophoresis

et al. 2002

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The novel antidepressant reboxetine, a selective norepinephrine reuptake inhibitor, is increasingly used in the treatment of different forms of major depression. Reboxetine is a chiral compound, and is marketed as a racemic mixture of (R,R)- and (S,S)-reboxetine; however, the pharmacokinetic and toxicological profiles of the two enantiomers are rather different. For this reason, a simple capillary electrophoretic method for the separation of reboxetine enantiomers has been developed. Sulfobutyl ether-beta-cyclodextrin was chosen as the chiral selector, and several parameters, such as cyclodextrin and buffer concentration, buffer pH and capillary temperature were investigated in order to obtain good separation and acceptable run times. Using an uncoated, fused-silica capillary (internal diameter 50 microm, total length 48.5 cm, effective length 40.0 cm) and a background electrolyte consisting of a pH 3.0, 100 mM phosphate buffer containing 1.25 mM cyclodextrin, reboxetine enantiomers were baseline separated (resolution > 4) with a voltage of 20 kV in less than 16 min. Since pure enantiomers of reboxetine were not available, they were obtained from the racemic powder by means of direct-phase, high-performance liquid chromatography and their identity confirmed by circular dichroism spectra.

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The effect of pore size of silica gel and concentration of buffer on capillary chromatographic and capillary electrochromatographic enantioseparations using cellulose tris(3,5-dichlorophenylcarbamate)

Cited by 43 publications

References 21 publications

Enantioseparations in nonaqueous capillary liquid chromatography and capillary electrochromatography using cellulose tris(3,5-dimethylphenylcarbamate) as chiral stationary phase

Enantioseparations in nonaqueous capillary liquid chromatography and capillary electrochromatography using cellulose tris(3,5-dimethylphenylcarbamate) as chiral stationary phase

Capillary electrochromatographic separation of enantiomers on chemically bonded type of cellulose derivative chiral stationary phases with a positively charged spacer

Separation of reboxetine enantiomers by means of capillary electrophoresis

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