The Effect of Pore Size Distribution on the Rate of Enzymatic Hydrolysis of Cellulosic Substrates

Grethlein, Hans E.

doi:10.1038/nbt0285-155

Cited by 482 publications

(312 citation statements)

References 9 publications

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“…Biomass saccharification via biological conversion involves two steps-lignocellulose pretreatment or fractionation followed by enzymatic cellulose (and perhaps hemicellulose) hydrolysis. Dilute acid pretreatment (DA), typically using sulfuric acid, is the most investigated pretreatment method (Bernardez et al, 1993;Grethlein, 1985;Grethlein and Converse, 1991;Lloyd and Wyman, 2005;Ooshima et al, 1990;Schell et al, 2003;Thompson et al, 1992). Conducted at relatively high temperatures (150-2008C) and pressures (120-200 psia), DA pretreatment solublizes acid-labile hemicellulose and thereby disrupts the lignocellulosic composite linked by covalent bonds, hydrogen bonds and van der Waals forces (Burns et al, 1989;Lloyd and Wyman, 2005;Ooshima et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

“…The total substrate accessibility has been measured previously by using cellulase-size molecule exclusion (Burns et al, 1989;Esteghlalian et al, 2001;Grethlein, 1985;Thompson et al, 1992), low-temperature cellulase adsorption (Gerber et al, 1997;Kumar and Wyman, 2008;Lee et al, 1994;Lu et al, 2002;Mooney et al, 1998) or labeled cellulase (Jeoh et al, 2007;Palonen et al, 2004). However, in pretreated lignocellulose materials it remains relatively challenging to quantitatively differentiate accessibilities for cellulose and non-cellulose fractions.…”

Section: Introductionmentioning

confidence: 99%

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Comparative study of corn stover pretreated by dilute acid and cellulose solvent‐based lignocellulose fractionation: Enzymatic hydrolysis, supramolecular structure, and substrate accessibility

Zhu

Sathitsuksanoh

Vinzant

et al. 2009

Biotech & Bioengineering

199

164

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Liberation of fermentable sugars from recalcitrant biomass is among the most costly steps for emerging cellulosic ethanol production. Here we compared two pretreatment methods (dilute acid, DA, and cellulose solvent and organic solvent lignocellulose fractionation, COSLIF) for corn stover. At a high cellulase loading [15 filter paper units (FPUs) or 12.3 mg cellulase per gram of glucan], glucan digestibilities of the corn stover pretreated by DA and COSLIF were 84% at hour 72 and 97% at hour 24, respectively. At a low cellulase loading (5 FPUs per gram of glucan), digestibility remained as high as 93% at hour 24 for the COSLIF-pretreated corn stover but reached only $60% for the DA-pretreated biomass. Quantitative determinations of total substrate accessibility to cellulase (TSAC), cellulose accessibility to cellulase (CAC), and non-cellulose accessibility to cellulase (NCAC) based on adsorption of a nonhydrolytic recombinant protein TGC were measured for the first time. The COSLIF-pretreated corn stover had a CAC of 11.57 m 2 /g, nearly twice that of the DA-pretreated biomass (5.89 m 2 /g). These results, along with scanning electron microscopy images showing dramatic structural differences between the DA-and COSLIF-pretreated samples, suggest that COSLIF treatment disrupts microfibrillar structures within biomass while DA treatment mainly removes hemicellulose. Under the tested conditions COSLIF treatment breaks down lignocellulose structure more extensively than DA treatment, producing a more enzymatically reactive material with a higher CAC accompanied by faster hydrolysis rates and higher enzymatic digestibility.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative study of corn stover pretreated by dilute acid and cellulose solvent‐based lignocellulose fractionation: Enzymatic hydrolysis, supramolecular structure, and substrate accessibility

Zhu

Sathitsuksanoh

Vinzant

et al. 2009

Biotech & Bioengineering

199

164

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“…Reid (1995) suggested that physical contact between enzyme and substrate is the rate-limiting step in lignin degradation. Grethlein (1985) indicated that substrate pretreatment (using dilute sulphuric acid in this example) is necessary to increase the number of available sites for cellulase action.…”

Section: Enzyme Access To Substratementioning

confidence: 99%

“…According to Tomme et al (1995) and Grethlein (1985) the accessibility of enzymes to wood and fibers is limited by factors such as adsorption to surface areas, low fiber porosity, and low median pore size of fibers. Reid (1995) suggested that physical contact between enzyme and substrate is the rate-limiting step in lignin degradation.…”

Section: Enzyme Access To Substratementioning

confidence: 99%

Lignocellulose biodegradation: Fundamentals and applications

Malherbe

Cloete

2002

Rev Environ Sci Biotechnol

398

163

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“…The critical parameters for alkaline pretreatment are reaction temperature, pretreatment time and alkali loading [13]. Apart from that, the accessibility of surface area, the presence of lignin and hemicelluloses would be the key factors affecting the rate of enzymatic hydrolysis of cellulose [14], [15].…”

Section: Introductionmentioning

confidence: 99%

Sodium Hydroxide Pretreatment and Enzymatic Hydrolysis of Oil Palm Mesocarp Fiber

Iberahim¹,

Jahim²,

Harun³

et al. 2013

IJCEA

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Abstract-Sodium hydroxide pretreatment of oil palm mesocarp fiber (OPMF) was carried out with NaOH from 2% to 10% (w/v) at temperature 50 0 C and 70 0 C. The performances of pretreatments were evaluated based on total carbohydrate and reducing sugar including glucose, xylose and arabinose after enzymatic hydrolysis on the pretreated biomass. It was found that the enzymatic hydrolysis had significantly improved when 6% NaOH in 70 0 C applied in the pretreatment process. The highest total reducing sugars produced by means of commercial enzymes was achieved with the overall conversions of glucan and xylan of 87% and 60.73% respectively. The compositions of OPMF in this study are as follows (% g/g dry biomass): glucan, 28.8, xylan, 25.3, arabinan, 1.91, ethanol extractive, 6.32 and ash, 2.60.Index Terms-Enzymatic hydrolysis, glucose, NaOH pretreatment, oil palm mesocarp fiber.

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The Effect of Pore Size Distribution on the Rate of Enzymatic Hydrolysis of Cellulosic Substrates

Cited by 482 publications

References 9 publications

Comparative study of corn stover pretreated by dilute acid and cellulose solvent‐based lignocellulose fractionation: Enzymatic hydrolysis, supramolecular structure, and substrate accessibility

Comparative study of corn stover pretreated by dilute acid and cellulose solvent‐based lignocellulose fractionation: Enzymatic hydrolysis, supramolecular structure, and substrate accessibility

Lignocellulose biodegradation: Fundamentals and applications

Sodium Hydroxide Pretreatment and Enzymatic Hydrolysis of Oil Palm Mesocarp Fiber

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