The effect of polyamines on protein kinase activities of wheat (Triticum aestivum) L. anthers

Abstract

Protein kinase activities were extracted from anther tissue of wheat (Triticum aestivum L. cv . Zaragoza) and characterized with regard to the effects of polyamines, second messengers and plant growth hormones . The protein kinases were inhibited by polyamines and cyclic nucleotides, but were stimulated by the addition of auxins, gibberellic acid and kinetin . The dominant polyamine-sensitive kinase activity was partially purified and characterized . The optimal pH of the reaction was 7 .5 to 8 .0 and casein was the preferred exogenous substrate. Polyamines were inhibiting in the decreasing order of putrecine > spermidine > spermine. The results are discussed against the context of the anther culture technique .

Effects of exogenous application of polyamines on wheat anther cultures

“…During androgenesis of Triticum aestivum (Bothma and Duberry 1991), Nicotiana tabacum, and Datura innoxia (Villanueva et al 1985), put and spd were found to be the major PAs, and could be relevant in anther culture. Recent studies with wheat have also shown genetic variability in androgenic traits with respect to embryo induction and plant regeneration (Liu et al 2002;Zheng 2003).…”

Interaction of spermine with a signal transduction pathway involving phospholipase C, during the growth of Catharanthus roseus transformed roots

“…A significant amount of radioactivity was found in soluble extracts (Table 2). In this case, the effect of Pas on enzymatic activities, fundamentally nuclear and cytosolic protein kinases, has been reported (Polya and Micucci 1985, Datta et al 1986, Bothma and Dubery 1991, Ye et al 1994, Chang and Kang 1999). However, it is very important to point out that in the events mentioned above, the effect was independent of the polyamine being used, indicating that the polyamines are acting as general charged agents.…”