The effect of Pleurotus ostreatus arabinofuranosidase and its evolved variant in lignocellulosic biomasses conversion

Marcolongo, Loredana; Ionata, Elena; Cara, Francesco La; Amore, Antonella; Giacobbe, Simona; Pepe, Olimpia; Faraco, Vincenza

doi:10.1016/j.fgb.2014.07.003

Cited by 27 publications

(24 citation statements)

References 24 publications

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“…Giacobbe et al (2016) tested the effect of this endo-cellulase in addition to the commercial preparation Cellic® Ctec3/Htec3 from Novozymes, finding that the glucan and xylan conversion using only commercial enzyme cocktail was higher when compared to that obtained upon supplementation with the purified endo-cellulase rCelstrep. Marcolongo et al (2014) observed a decrease up to 50% in glucose and xylose yield by using the purified rCelStrep in substitution to Accellerase® 1500 in the same mixture of commercial enzymes from Genencor used in our work. These results were lower than that obtained in the present work by the substitution of the enzymatic extract from S. argenteolus AE58P to the Accellerase® 1500.…”

Section: Discussionmentioning

confidence: 55%

“…Probably the cellulolytic activity from S. argenteolus AE58P worked better in combination with the Genencor commercial cocktail than those produced by the other strains. Furthermore, it is possible that a higher glucan deconstruction also had a positive impact on the hydrolysis of xylan, which is strictly absorbed on cellulose fibrils (Dammstrom et al, 2009; Marcolongo et al, 2014). In order to improve the glucose yield, double amounts of each biocatalyst was utilized (Mix 2A, 3A, 4A, and 5A) without achieving better results for glucose yields.…”

Section: Discussionmentioning

confidence: 99%

“…To the best of our knowledge, the ability of only one endo-cellulase from Streptomyces strain (named rCelStrep from Streptomyces sp. G12, after recombinant expression in E. coli and purification) was previously tested in saccharification of pretreated A. donax (Marcolongo et al, 2014; Giacobbe et al, 2016). Giacobbe et al (2016) tested the effect of this endo-cellulase in addition to the commercial preparation Cellic® Ctec3/Htec3 from Novozymes, finding that the glucan and xylan conversion using only commercial enzyme cocktail was higher when compared to that obtained upon supplementation with the purified endo-cellulase rCelstrep.…”

Section: Discussionmentioning

confidence: 99%

“…Probably better results would be obtained after the removal of the soluble compounds (e.g., sugar, sugar oligomers, sugar degradation products, and lignin-derived compounds) with inhibitory effects on the cellulose digestibility, contained in the slurry of the pretreated biomass. As a matter of fact, higher yields represented by 43 and 70% of glucose and xylose recovery respectively after 72 h of incubation were reached with the same mixture of commercial enzymes from Genencor applied to A. donax pretreated by steam explosion but devoid of the liquid fraction containing soluble matter (Marcolongo et al, 2014). …”

Section: Discussionmentioning

confidence: 99%

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Lignocellulose-Adapted Endo-Cellulase Producing Streptomyces Strains for Bioconversion of Cellulose-Based Materials

et al. 2016

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Twenty-four Actinobacteria strains, isolated from Arundo donax, Eucalyptus camaldulensis and Populus nigra biomass during natural biodegradation and with potential enzymatic activities specific for the degradation of lignocellulosic materials, were identified by a polyphasic approach. All strains belonged to the genus Streptomyces (S.) and in particular, the most highly represented species was Streptomyces argenteolus representing 50% of strains, while 8 strains were identified as Streptomyces flavogriseus (synonym S. flavovirens) and Streptomyces fimicarius (synonyms Streptomyces acrimycini, Streptomyces baarnensis, Streptomyces caviscabies, and Streptomyces flavofuscus), and the other four strains belonged to the species Streptomyces drozdowiczii, Streptomyces rubrogriseus, Streptomyces albolongus, and Streptomyces ambofaciens. Moreover, all Streptomyces strains, tested for endo and exo-cellulase, cellobiase, xylanase, pectinase, ligninase, peroxidase, and laccase activities using qualitative and semi-quantitative methods on solid growth medium, exhibited multiple enzymatic activities (from three to six). The 24 strains were further screened for endo-cellulase activity in liquid growth medium and the four best endo-cellulase producers (S. argenteolus AE58P, S. argenteolus AE710A, S. argenteolus AE82P, and S. argenteolus AP51A) were subjected to partial characterization and their enzymatic crude extracts adopted to perform saccharification experiments on A. donax pretreated biomass. The degree of cellulose and xylan hydrolysis was evaluated by determining the kinetics of glucose and xylose release during 72 h incubation at 50°C from the pretreated biomass in the presence of cellulose degrading enzymes (cellulase and β-glucosidase) and xylan related activities (xylanase and β-xylosidase). The experiments were carried out utilizing the endo-cellulase activities from the selected S. argenteolus strains supplemented with commercial β-gucosidase and xylanase preparations from Genencore (Accellerase BG and Accellerase XY). Cellulose and xylan conversion, when conducted using commercial (hemi)cellulases, gave glucose and xylose yields of 30.17 and 68.9%, respectively. The replacement of the cellulolytic preparation from Genencor (Accellerase 1500), with the endo-cellulase from S. argenteolus AE58P resulted in almost 76% of the glucose yield obtained in the presence of the commercial counterpart. Due to the promising results obtained by using the enzymatic crude extracts from S. argenteolus AE58P in the pretreated A. donax saccharification experiments, the proteins putatively responsible for endo-cellulase activity in this strain were identified by proteomics. Several proteins were confidently identified in different Streptomyces spp., eight of which belong to the class of Carbohydrate active enzymes. Overall results highlighted the biotechnological potential of S. argenteolus AE58P being an interesting candidate biocatalyst-producing bacterium for lignocellulose conversion and production of biochemicals and bioenergy.

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Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Lignocellulose-Adapted Endo-Cellulase Producing Streptomyces Strains for Bioconversion of Cellulose-Based Materials

et al. 2016

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“…However, only a limited number of studies on synergism of a-arabinofuranosidases on hydrolysis of lignocellulosic substrates have been reported. These included the enhancement of xylose recovery by arabinofuranosidase mutant from Pleutus ostreatus on hydrolysis of pretreated Arundo donax, corn cobs, and brewer's spent grains (Marcolongo et al, 2014), synergistic effects of Penicillium arabinofuranosidase on hydrolysis of rice straw by cellulase and xylanase (Lee et al, 2011), and the combination of a-arabinofuranosidase to mannanase and xylanase on hydrolysis of bagasse (Beukes and Pletschke, 2010). In addition to the action of hydrolytic enzymes, PET can also further increase the efficiency of the enzyme systems by de-esterification of the pectin component, which acts as the embedded matrix in the cell wall structure.…”

Section: Cooperative Enzyme Interaction On Pretreated Rice Strawmentioning

confidence: 99%