The effect of platelet activating factor on different phases of murine in vitro fertilization

Shi, Hualin; Miller, F.; Miller, Kathleen B.; Kim, Moon H.

doi:10.1007/bf01203962

Cited by 12 publications

(3 citation statements)

References 12 publications

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“…This characteristic of albumin‐dependent release is apparently coupled with the spontaneous and reversible induction of sperm capacitation, because albumin is not generally recognized as a signaling molecule. In vitro capacitation induced by PAF has been further demonstrated in spermatozoa of the mouse model (Sengoku et al, 1992; Shi et al, 1992; Huo et al, 2000; Wu et al, 2001), the bovine model (Aravindakshan and Sharma, 1995), the stallion model (Odeh et al, 2003), and humans (Wu et al, 2001; Henkel and Schill, 2003).…”

Section: Discussionmentioning

confidence: 99%

High Seminal Platelet‐Activating Factor Acetylhydrolase Activity in Men With Spinal Cord Injury

Zhu

Brackett

Aballa

et al. 2006

Journal of Andrology

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Spinal cord injury (SCI) causes male infertility, with low sperm motility the major long-term cause. It has been suggested in previous studies that some seminal components may be responsible for the pathological asthenozoospermia. It is hypothesized that platelet-activating factor (PAF) acetylhydrolase (PAFah), which originates in the epididymis and other accessory sexual glands, may be a causative factor. This enzyme catalyzes PAF to acetate and biologically inactive lyso-PAF. PAF is well recognized to be an important phospholipid mediator that stimulates sperm motility and enhances sperm capacitation and fertilization. The present study was designed to analyze differences in PAFah activity in semen of men with SCI and age-matched healthy men. PAFah assay reagent kits were used to measure enzymatic activity by monitoring the production rates of 4-nitrophenol on a spectrophotometer during a given interval. The results showed that subjects with SCI had a higher concentration of PAFah than men in the control group (P , .001). A statistically significant negative correlation was found between enzymatic activity and sperm motility (r 2 5 0.8449; P , .001). Further studies will determine whether seminal vesicle dysfunction in men with SCI leads to abnormal PAFah activity, resulting in low sperm motility.

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Section: Discussionmentioning

confidence: 99%

High Seminal Platelet‐Activating Factor Acetylhydrolase Activity in Men With Spinal Cord Injury

Zhu

Brackett

Aballa

et al. 2006

Journal of Andrology

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“…The focus of the vast majority of these studies has been to observe and analyze the changes in spermatozoal motility and/or biochemical properties in the presence of exogenously added PAF. Upon incubation, effects of PAF on motility (Ricker et al, 1989;Hellstrom et al, 1991;Jarvi et al, 1993b), acrosome reactivity Angle et al, 1993;Fukuda et al, 1994;Krausz et al, 1994;Luconi et al, 1995), and fertilization potentials in vitro (Kuzan et al, 1990;Shi et al, 1992;Angle et al, 1993;Fukuda et al, 1994;Minhas, 1993;Sengoku et al, 1993) have been observed.…”

Section: Paf In Semenmentioning

confidence: 99%

“…Although interesting, care must be used in drawing conclusions from such experiments. While some of these studies involve more ''physiological'' concentrations (low nM range) (Sengoku et al, 1993;Krausz et al, 1994), other effects were observed at higher concentrations of PAF (high nM to high µM range) Shi et al, 1992;Jarvi et al, 1993b;Minhas, 1993;Fukuda et al, 1994). The nanomolar range might even be an overestimation in semen since (Grigg et al, 1996) a Sequenced, either partially or completely.…”

Section: Paf In Semenmentioning

confidence: 99%

Platelet-activating factor acetylhydrolase in seminal plasma: A brief review

Soubeyrand

Manjunath

1998

Mol. Reprod. Dev.

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Effects of platelet activating factor on capacitation and acrosome reaction in mouse spermatozoa

and

Yang

2000

Mol. Reprod. Dev.

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Platelet activating factor (PAF) plays an important role in mammalian reproduction. The aim of this study was to investigate the effects of PAF on capacitation and acrosome reaction of mouse spermatozoa by chlortetracycline (CTC) fluorescence assay and coomassie blue staining. The percentage of capacitated mouse spermatozoa was increased (P < 0.05) by incubation with 50 ng/ml PAF for 20–120 min. The peak response occurred between 80 to 100 min of exposure to PAF. In contrast, the effects of PAF on acrosome reaction may be not receptor‐mediated since lyso‐PAF had the same effects. Ionophore A23187 stimulated an increase in acrosome‐reacted spermatozoa of PAF‐treated spermatozoa, but not of lyso‐PAF‐treated ones. These results suggest that PAF mainly acts on sperm capacitation. Mol. Reprod. Dev. 56:436–440, 2000. © 2000 Wiley‐Liss, Inc.

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The effect of platelet activating factor on different phases of murine in vitro fertilization

Abstract: The PAF concentration of 10(-6) M in sperm capacitation and sperm/oocyte coincubation yielded the greatest improvement in fertilization. However, continuing PAF treatment after sperm/oocyte coincubation had no beneficial effect on fertilization and preimplantation development.

Cited by 12 publications

References 12 publications

High Seminal Platelet‐Activating Factor Acetylhydrolase Activity in Men With Spinal Cord Injury

High Seminal Platelet‐Activating Factor Acetylhydrolase Activity in Men With Spinal Cord Injury

Platelet-activating factor acetylhydrolase in seminal plasma: A brief review

Effects of platelet activating factor on capacitation and acrosome reaction in mouse spermatozoa

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