The effect of plasma-induced graft copolymerization of PHEMA on silicone rubber towards improving corneal epithelial cells growth

Hsiue, Ging‐Ho; Lee, Shyh-Dar; Wang, Chee-Chan; Chang, Patricia Chuen-Tsuei

doi:10.1163/156856293x00302

Cited by 18 publications

(4 citation statements)

References 10 publications

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“…Moreover, the SR-g-pHEMA (210 pg/cm2) surface inhibits CE cell migration, attachment, and proliferation. However, this difference between SR-g-pHEMA (210 pg/cm2) and SRg-pHEMA (75 pg/cm2) for biological properties was explained by the hydrogel surface being provided in SR-g-pHEMA (210 pg/cm2) but a hydrophilic oligomer segment existing in the SR-g-pHEMA (75 pg/ cm2) (30). The satisfactory results of in vitro evaluation for the biocompatibility of SR-g-pHEMA (75 pg/cm2 and 210 pg/cm2) and SR-g-pAA-PEO allowed us to proceed to the in vivo study.…”

Section: Biological Analysismentioning

confidence: 98%

Surface Modification of Silicone Rubber Membrane by Plasma Induced Graft Copolymerization as Artificial Cornea

1996

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In this study a highly biocompatible polymer membrane was prepared by surface modification. An artificial cornea was also developed for clinical applications. Silicone rubber (SR) membrane was grafted with hydrophilic monomers such as 2‐hydroxyethyl methacry‐late (HEMA) and acrylic acid by plasma induced grafted polymerization. Surface properties of the SR were characterized using secondary ions mass spectra, Fourier transform infrared/attenuated total reflection, and element spectra for chemical analysis. The corneal epithelial (CE) cell was cultured in vitro, and penetrating keratoplasty of albino rabbit cornea (in vivo) was performed to evaluate biological properties of modified SR membranes. The ability of the CE cell to attach onto various SR membranes was observed by inverted microscopy. The proliferation of CE cell was conducted in approximately 96 h. Experimental results indicated that the attachment and growth of CE onto SR‐g‐pHEMA (75 μg/ cm2) is enhanced. The morphologies of an attached CE cell are similar to those of a primary CE cell. In the in vivo study, the depth of anterior chamber was maintained 2 weeks after penetrating keratoplasty was performed with a SR grafted with pHEMA (210 μg/cm2). This phenomenon displayed a high biocompatibility of modified SR membrane with the CE cell. Furthermore, results in this study provide a valuable reference for application of the modified SR for an artificial cornea.

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Section: Biological Analysismentioning

confidence: 98%

Surface Modification of Silicone Rubber Membrane by Plasma Induced Graft Copolymerization as Artificial Cornea

1996

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“…The surface properties are altered by the plasma exposure, i.e., to be less thrombogenic in nature. In graft polymerization a definite thickness on the surface is modified with significant alteration in the surface morphology depending on the degree of grafting (Hsiue et al, 1993;Sharma et al, 1987).…”

Section: Plasma Modificationmentioning

confidence: 99%

Bulk and/or Surface Modification of Medical Polymers to Attain Antimicrobial Activity and/or Biocompatibility

Shintani¹

2005

Biocontrol Sci.

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In recent years, there has been a great demand for polymers designed with better functions that include blood and tissue compatibility (biocompatibility) and an antimicrobial activity. The surface modification of existing polymers is one of the most attractive ways to accomplish this. Treatment with plasma, gamma ray or electron beam leads to a sufficient level of activation of polymers so that specific monomers may be grafted. The extent of modification, i.e., the degree of grafting, may be easily controlled by the careful manipulation of the radiation exposure and reaction conditions. Graft modification may be carried out almost on all polymers with the creation of a desired functional chemistry both on the surface and in the bulk matrix. The grafting is applicable to almost all polymer-monomer combinations with enormous possibilities regarding physicochemical and biological characteristics to attain appropriate antimicrobial activity as well as suitable biocompatibility.

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“…Neste trabalho foram utilizadas a espectroscopia em infravermelho, a microscopia eletrônica de varredura e o teste de hidrofilicidade. Há vários trabalhos que utilizaram a espectroscopia para análise de copolímeros de enxerto, dentre eles os de Hisue e col. (1992 e 1994) e Lee e col. (1996) [52][53][54] .…”

Section: Caracterização Dos Biomateriaisunclassified

“…estudaram extensivamente a aplicação de hidrogéis poliméricos em diferentes substratos e suas correspondentes propriedades de biocompatibilidade [44] . Hisue e col. [52][53] e Lee e col. [54] estudaram a enxertia do PHEMA em borracha de silicone através da enxertia induzida por plasma para a obtenção de córneas artificiais.…”

Section: Imobilização Da Toxina Marcadaunclassified

"Obtenção dos sistemas bioconjugados Crotoxina/PEBD-g-Phema e Crotoxina/PCL"

Lorenzetti¹

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The effect of plasma-induced graft copolymerization of PHEMA on silicone rubber towards improving corneal epithelial cells growth

Cited by 18 publications

References 10 publications

Surface Modification of Silicone Rubber Membrane by Plasma Induced Graft Copolymerization as Artificial Cornea

Surface Modification of Silicone Rubber Membrane by Plasma Induced Graft Copolymerization as Artificial Cornea

Bulk and/or Surface Modification of Medical Polymers to Attain Antimicrobial Activity and/or Biocompatibility

"Obtenção dos sistemas bioconjugados Crotoxina/PEBD-g-Phema e Crotoxina/PCL"

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