The effect of plant growth regulators and natural supplements on in vitro propagation of Pogostemon cablin Benth.

Swamy, Mallappa Kumara; Mohanty, Sudipta Kumar; Anuradha, M.

doi:10.1007/s12892-013-0038-1

Cited by 35 publications

(27 citation statements)

References 22 publications

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“…This differed from the results of Fadel et al (2010), who reported greater numbers of roots at a 50% MS salt concentration with Menthas picata L., while a 25% MS concentration had an inhibitory effect on root formation. Kumaraswamy and Anuradha, (2010) and Swamy et al (2014) reported similar results with P. cablin (the O. basilicum family). Dahab et al (2005) obtained greater numbers of roots in Ruscus poglossum L. at a 50% MS concentration combined with 2.0 mg/L AIB, and longer roots at a 25% MS concentration combined with 3.0 mg/L AIB.…”

Section: Test 1: Effects Of the Ms Salts On The Growth Of Purple Basilsupporting

confidence: 56%

“…Charcoal is commonly used in tissue culture procedures, and Ekmekci and Aasim (2014) reported greater growth of the aerial portion and enhanced rooting of O. basilicum with charcoal and polyvinyl pyrrolidone (PVP) added to the MS medium. For species of the same family as basil, antioxidant substances added to the culture medium have been observed to favor seedling development, principally activated charcoal and PVP (Kumaraswamy and Anuradha, 2010;Swamy et al, 2014) The presence of callus was observed in 100% of the seedlings that developed in MS medium without charcoal (Fig 3 A and D), indicating that charcoal was essential to diminishing callus formation. This result indicates that callus formation in 'red rubin' basil is related to increased uptakes of metals (such as copper) from the media that can generate free radicals that cause stress (Posmyk et al, 2009).…”

Section: Test 2: Effects Of Charcoal and Sucrose On The Growth Of Purmentioning

confidence: 99%

“…Studies have demonstrated that when MS salt concentrations are halved, species of the Lamiaceae family show increases in root numbers and sizes (Fadel et al, 2010;Kumaraswamy and Anuradha, 2010;Swamy et al, 2014). MS medium has a high osmotic potential due its salt and sucrose concentrations (George et al, 2008), and when salt concentrations are reduced the osmotic potential likewise decreases, which contributes to more efficient water absorption by the roots.…”

Section: Test 1: Effects Of the Ms Salts On The Growth Of Purple Basilmentioning

confidence: 99%

“…P.cablin, a species belonging to the Family Lamiaceae, showed 93% rooting when grown in a half dosage of MS salts and 100 mg/L activated charcoal (Kumaraswamy and Anuradha, 2010;Swamy et al 2014). Seedlings of O. basilicum, a common green variety, developed well, without callus formation, in a medium enriched with 1 mg/L polyvinyl pyrrolidone (PVP) and 3.0 mg/L activated charcoal (Ekmekci and Aasim, 2014).…”

Section: Introductionmentioning

confidence: 99%

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In vitro cultivation of purple basil Ocimum basilicum L. ‘red rubin’ at different levels of salts, charcoal, sucrose and potassium iodine

Silva¹,

Nascimento²,

Barbosa³

et al. 2017

Aust J Crop Sci

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Ocimum basilicum'red rubin' is highly valued for its essential oil, and is widely used in folk medicine, but few studies examining in vitro propagation techniques for this basil variety are available. We examined the development of red rubin basil seedlings grown in vitro in different concentrations of mineral salts, charcoal combined with sucrose, and potassium iodine in three series of tests. In the first series, five concentrations (0, 25, 50, 75 and 100%) of Murashige and Skoog salts (MS culture medium) were used to germinate sterilized seeds of O. basilicum. In the second test, to evaluate the combined effects of charcoal and sucrose on basil growth, three concentrations of charcoal (0, 3.0, and 4.5 g/L) and two of sucrose (30 and 60 g/L) were used. In the third test, the seeds were sown onto culture medium enriched with five different concentrations of potassium iodine (0, 25, 50, 75 and 100µM); 0.5 μM benzyl aminopurine (BAP) and 1.0 μM naphthalene acetic acid (NAA) were added to all germination media. A completely randomized experimental design was used in all tests, with five replicates, four vials per test, and 4 seeds per vial. In Test 2, seedling oxidation (%) and the numbers of leaves and shoots were submitted to non-parametric analysis using the Kruskal Wallis test (p ≤0.05). The other data were submitted to analysis of variance (p≤0.05). The qualitative means were evaluated by the Tukey test (p≤0.05), and the quantitative means by polynomial regression (p≤0.05). The results showed that 100% MS culture medium was most indicated for 'red rubin' seed germination and aerial portion production, while 70-80% MS was indicated for root production. A sucrose level of 30 g/L (standard) combined with 4.5 g/L of activated charcoal allowed the best seedlings development and inhibited callus formation. Activated charcoal limited copper and zinc uptake by O. basilicum seedlings, but not enough to hamper their development. Increasing concentrations of potassium iodine reduced all of the growth variables analyzed in this basil variety.Keywords: Ocimum basilicum L., micropropagation, medicinal plant, culture medium. Abbreviation: AC_ activated charcoal, APL_ aerial portion length, BAP_ benzylaminopurine, Cu_ Copper; CSD1 and CSD2_ gene families in Arabidopsis, DMAP_ dry mass of the aerial portion, DMRC_ dry mass of the root or of the root + callus portion, FMAP_ fresh masses of the aerial portions, FMRC_ fresh masses of the root, KI_ potassium iodide, miRNAs_ micro RNAs, MS_ Murashige and Skoog, NAA_ naphthalene acetic acid, NC-presence of callus, NL_ number of leaves, NS_ numbers of shoots, OX_ oxidized plants, PVP_polyvinyl pyrrolidone, RL_ root, SISVAR_ statistical analysis system, SOD_ superoxide dismutase, Zn_ Zinc.

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Section: Test 1: Effects Of the Ms Salts On The Growth Of Purple Basilsupporting

confidence: 56%

Section: Test 2: Effects Of Charcoal and Sucrose On The Growth Of Purmentioning

confidence: 99%

Section: Test 1: Effects Of the Ms Salts On The Growth Of Purple Basilmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro cultivation of purple basil Ocimum basilicum L. ‘red rubin’ at different levels of salts, charcoal, sucrose and potassium iodine

Silva¹,

Nascimento²,

Barbosa³

et al. 2017

Aust J Crop Sci

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show abstract

“…A specific strategy is required to produce active principles from in vitro cultured cells [7,96]. Some of the factors that influence the in vitro culture includes type of the explants, media composition, type of plant growth regulators, different growth conditions (temperature, light sources, and humidity), types of cultures (solid cultures and agitated liquid cultures), cell line section, and the use of elicitation technology [7,96,97]. In vitro plant regeneration can be obtained through direct organogenesis or indirect organogenesis, which involves callus interphase [91,93,94].…”

Section: Biotechnological Aspects Of L Reticulatamentioning

confidence: 99%

<em>Leptadenia reticulata</em> (Retz.) Wight (Jivanti): Botanical, Agronomical, Phytochemical, Pharmacological, and Biotechnological Aspects

Mohanty¹,

Swamy²,

Sinniah³

et al. 2017

Preprint

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Leptadenia reticulata (Ritz.) Wight (Asclepiadaceae), a traditional medicinal plant species, is widely used in traditional medicine to treat various ailments such as tuberculosis, hematopoiesis, emaciation, cough, dyspnea, fever, burning sensation, night blindness, cancer, and dysentery. In Ayurveda, it is known for its revitalizing, rejuvenating, and lactogenic properties. This plant is one of the major ingredients in many commercial herbal formulations, including Speman, Envirocare, Calshakti, Antisept, and Chyawanprash. The therapeutic potential of this herb is because of the presence of diverse bioactive compounds such as α-amyrin, β-amyrin, ferulic acid, luteolin, diosmetin, rutin, β-sitosterol, stigmasterol, hentriacontanol, a triterpene alcohol simiarenol, apigenin, reticulin, deniculatin, and leptaculatin. However, most biological studies on L. reticulata are restricted to crude extracts, and many biologically active compounds are yet to be identified in order to claim the traditional uses of L. reticulata into evidence-based uses. At present, L. reticulata is a threatened endangered plant because of overexploitation, unscientific harvesting, and habitat loss. The increased demand from pharmaceutical, nutraceutical, and veterinary industries has prompted its large-scale propagation. However, its commercial cultivation is hampered because of the non-availability of genuine planting material and the lack of knowledge on its agronomical practices. In this regard, micropropagation technique will be useful to obtain true-to-type L. reticulata planting materials from an elite germplasm to meet the current demand. Adopting other biotechnological approaches such as synthetic seed technology, cryopreservation, cell culture, and genetic transformation can warrant conservation as well as increased metabolite production from L. reticulata. The present review summarizes scientific information on the botanical, agronomical, phytochemical, pharmacological, and biotechnological aspects of L. reticulata. This comprehensive information will certainly allow better utilization of this industrially important herb towards the discovery of lead drug molecules.

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Anticancer Plants and Their Conservation Strategies: An Update

Kumar

Swamy

Akhtar³

2018

Anticancer Plants: Properties and Application

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The effect of plant growth regulators and natural supplements on in vitro propagation of Pogostemon cablin Benth.

Cited by 35 publications

References 22 publications

In vitro cultivation of purple basil Ocimum basilicum L. ‘red rubin’ at different levels of salts, charcoal, sucrose and potassium iodine

In vitro cultivation of purple basil Ocimum basilicum L. ‘red rubin’ at different levels of salts, charcoal, sucrose and potassium iodine

<em>Leptadenia reticulata</em> (Retz.) Wight (Jivanti): Botanical, Agronomical, Phytochemical, Pharmacological, and Biotechnological Aspects

Anticancer Plants and Their Conservation Strategies: An Update

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