The effect of plant growth regulators on somaclonal variation in Cavendish banana (Musa AAA cv. ‘Zelig’)

Study of Genetic and Phenotypic Variability among Somaclones Induced by BAP and TDZ in Micropropagated Shoot Tips of Banana (Musa spp.) Using RAPD Markers

“…5) mother plants of AAA groups were placed together and their regenerated somaclones were placed away from their parental cultivars, as somaclone 9 from 'Berangan Intan' and somaclone 17 from 'Berangan' showed least similarity meanwhile, somaclone 28 from 'Rastali' (AAB) showed least similarity to all other. From each dendrogram caused by BAP and TDZ, it could be concluded that regenerated somaclones by higher concentrations of both BAP and TDZ accumulated much genetic variation compared to control and lower concentrations of each BAP and TDZ which supports the earlier report in banana cultivars (Bairu et al, 2006) based on positive correlation between concentration of cytokinins and frequency of genetic variation. 8,9,10,16,17,18,19,25,26,27 and 28 are micropropagated somaclones of cultivars 'Berangan Intan', 'Berangan' and 'Rastali' respectively according to Table 2. …”

“…This significant reduction in percentage of polymorphism reported by these authors could be due to the only one concentration of BAP applied in the medium in a way that this concentration has been previously approved as optimum BAP concentration for banana micropropagation (Kalimuthu et al, 2007;Venkatachalam et al, 2007;Bairu et al, 2008;and Shirani et al, 2009) The effect of BAP on genetic variation has been previously reported for other cultivars of banana (Bairu et al, 2006;Martin et al, 2006;Ray et al, 2006;EL-Dougdoug et al, 2007;Mohamed, 2007;and Sheidai et al, 2008). Bairu et al (2006) studied the correlation between multiplication rate and somaclonal variation, their results showed that treatments with high multiplication rate caused more variation. El-Dougdoug et al (2007) recorded 25% polymorphism among in vitro micropropagated banana which was closer to the findings reported in this study.…”

“…Somaclonal variation results in the production of new genotypes with a limited change in the original genome and molecular markers such as RAPD, RFLP, AFLP and microsatellites are ideal to identify genetic and epigenetic somaclones (Jain, 2001). Somaclonal variation has been studied to assess the potential of variability among the regenerated shoots of banana cultivars (Israeli et al, 1991;Sahijram et al, 2003;Bairu et al, 2006;Harirah and Khalid, 2006;Martin et al, 2006;Ray et al, 2006;El-Dougdoug et al, 2007;Jain et al, 2007;Mohamed, 2007;Oh et al, 2007;Venkatachalam and Sreedhar, 2007;and Sheidai et al, 2008). Although somaclonal variation is undesirable in the case of micropropagation as its primary objectives is to produce true -to -type clonal planting material, it can be useful for genetic improvement of banana (Sahijram et al, 2003;and Hwang, 2004).…”

DNA methylation changes in triticale due to in vitro culture plant regeneration and consecutive reproduction

“…Alternatively, the tissue culture medium composition could be also responsible for the difference in global DNA methylation. Plant growth regulators may enhance in vitro induced variation resulting from epigenetic modifications (Nehra et al 1992;Bairu et al 2006). Study on Malus xiaojinensis revealed increased DNA methylation with increasing 2,4-D (Huang et al 2012).…”

Micropropagation and Assessment of Genetic Stability of Musa sp. cv. Williams using RAPD and SRAP Markers.

“…RAPD analysis used to evaluate genetic stability of several micropropagated crops (Farahani et al, 2011 andGoyal et al, 2015). Two important factors were found to affect genetic stability in micropropagation prosses; the subculture number and subculture interval (Etienne &Bertrand, 2003 andBairu et al, 2006). …”