Micropropagation and Assessment of Genetic Stability of Musa sp. cv. Williams using RAPD and SRAP Markers.

Khatab, Ismael A.; Youssef, Mohamed S.

doi:10.21608/ejbo.2018.3199.1161

Cited by 11 publications

(9 citation statements)

References 20 publications

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“…The metabolites with the lowest PC2 loadings (more abundant in DPs than in NPs) were 1,2,3-Propanetricarboxylic acid 2-hydroxi, 2-Keto-d-gluconic acid, Psicofuranose and Propanoic acid, whereas D-Galactose 2-deoxy, D-Fructose, D-(-)-Fructofuranose and β-L-Arabinopyranose showed the highest PC2 loadings (more abundant in NPs compared to DPs) (Figure 3B). (11)(12)(13)(14)(15)(16)(17)(18)(19) are also visualized as an assessment set. Metabolites numbers in the loading plot correspond to Table 1.…”

Section: Overall Metabolite Profilingmentioning

confidence: 99%

“…However, the recognition of phenotypic characteristics and diagnosis of dwarfism can only be achieved by trained personnel at the end of the greenhouse acclimatization phase or in the field [8]. Molecular techniques such as random amplified polymorphic DNA (RAPD) [7,[16][17][18] amplified fragment length polymorphisms (AFLPs) [19] and inter simple sequence repeats (ISSRs) [17,[20][21][22][23] have been proposed to monitor SV in Musa. However, the relationship among the proposed molecular markers and the SVs was not described.…”

Section: Introductionmentioning

confidence: 99%

“…Williams microplants at the Rooting Phase coming from meristem M1 (N1-N10: normal plants, D1-D10: dwarf plants), for which the PCA was performed. Additionally, the plants M2 DP(11)(12)(13)(14)(15)(16)(17)(18)(19) are also visualized as an assessment set. Metabolites numbers in the loading plot correspond to Table1.…”

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confidence: 99%

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Changes in the Metabolite Profile during Micropropagation of Normal and Somaclonal Variants of Banana Musa AAA cv. Williams

et al. 2021

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Micropropagation techniques allow the mass production of banana plants but can cause somaclonal variations such as dwarfism. Changes in the metabolite profile during micropropagation of normal (NP) and dwarf (DP) banana plants have not been described. Both, NPs and DPs of banana Musa AAA cv. Williams were micropropagated and the metabolite profile of vitroplants was assessed at the proliferation (PP), rooting (RP) and the second greenhouse-acclimatization (APII) phases of tissue culture. Metabolites from 10 DPs and 10 NPs meristems from each micropropagation phase were extracted and identified by gas chromatography coupled with mass spectrometry (GC-MS). Principal component analysis (PCA) and test of statistical significance were applied to detect differentially accumulated metabolites. The PCA showed a clear grouping of DPs separated from NPs in RP and APII. Among the differentially accumulated metabolites, various precursors of apoplast components including arabinose and galactose or deoxygalactose in both PP and RP, as well as mannose and fucose in APII were under-accumulated in DPs. Results suggest affected apoplast composition during micropropagation of DPs.

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Section: Overall Metabolite Profilingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Changes in the Metabolite Profile during Micropropagation of Normal and Somaclonal Variants of Banana Musa AAA cv. Williams

et al. 2021

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“…Application of molecular analysis of in vitro regenerated plants has been well documented by many workers [27,28,29,30]. Molecular analysis is an efficient and reliable screening technique for tissue culture-derived plants [31,32]. Reliable monitoring of variability in DNA sequences of plants has been achieved using several PCR based molecular markers such as RAPD, ISSR, SSR and AFLP.…”

Section: Molecular Marker (Rapd-pcr)mentioning

confidence: 99%

Biometric and Genetic Stability Assessment of In Vitro Rooted Populus alba L. Micro-Shoots

Ahmed

Tawfik

2021

Preprint

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Populus alba is a deciduous large woody tree. The plant was introduced to shooting, then rooting multiplication on MS media. This work's design depends on two factors (media power and sucrose concentrations) with different concentrations resulted in 12 treatments. There were nine responses measured in the new individuals. Some morphological parameters had a significant difference, such as shoot length, leaf and root numbers. Where in the physiological measurements, all the parameters measured had significant differences. RAPD-PCR molecular marker was applied to assess the variation in the new individuals' genetic stability from treatments with the two factors. Four RAPD primers gave reproducible bands and resulted in a total polymorphism percentage of 38.33%. The new individuals resulted from the tissue culture technique were identical to the mother plant and each other. However, in this work, the use of different media power and sucrose concentrations resulted in different values in morphological, physiological responses, and P. alba's molecular variation.

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“…Genetic variations under long term culture in stevia and other plant species may be due to DNA hypermethylation (Rival et al, 2013), changes in chromosomal number and structure, pre-existed genetic changes in the donor plant or other reasons (Isah, 2015;Khatab & Youssef, 2018). The combination between shorten of subculture period and application of anti-ethylene improved shoot health through the disappearance of the vitrification phenomenon up to the sixth subculture.…”

Section: Jm Salemmentioning

confidence: 99%

Effects of Anti-ethylene Compounds on Vitrification and Genome Fidelity of Stevia rebaudiana Bertoni

Salem

2020

Egypt. J. Bot.

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Micropropagation and Assessment of Genetic Stability of Musa sp. cv. Williams using RAPD and SRAP Markers.

Cited by 11 publications

References 20 publications

Changes in the Metabolite Profile during Micropropagation of Normal and Somaclonal Variants of Banana Musa AAA cv. Williams

Changes in the Metabolite Profile during Micropropagation of Normal and Somaclonal Variants of Banana Musa AAA cv. Williams

Biometric and Genetic Stability Assessment of In Vitro Rooted Populus alba L. Micro-Shoots

Effects of Anti-ethylene Compounds on Vitrification and Genome Fidelity of Stevia rebaudiana Bertoni

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