The effect of physical stress on plant cells in suspension cultures

Tanaka, Hideo; Semba, Hisashi; Jitsufuchi, Tetsuya; Harada, Hiroshi

doi:10.1007/bf01027061

Cited by 53 publications

(19 citation statements)

References 6 publications

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“…The addition of conditioned medium to MS-production medium did result initially in the formation of a gelatinous precipitate complicating analysis. The enhancing effects of MS + C medium was initially assumed to result from the reduction of certain ions with precipitation or to the presence of a polysaccharide released from the plant cell wall (Tanaka et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

The effect of inoculum density and conditioned medium on the production of ajmalicine and catharanthine from immobilizedCatharanthus roseus cells

Lee

Shuler

2000

Biotechnol. Bioeng.

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Section: Discussionmentioning

confidence: 99%

The effect of inoculum density and conditioned medium on the production of ajmalicine and catharanthine from immobilizedCatharanthus roseus cells

Lee

Shuler

2000

Biotechnol. Bioeng.

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“…Some adaptation period may be necessary in some cases because cells may suffer a hydrodynamic stress generated by aeration and agitation to which cells are exposed (Tanaka et al, 1988). However, after adaptation, very proliferative, health cells grew fast in the solution (Figure 2).…”

Section: Cell Suspension Formationmentioning

confidence: 99%

“…Regardless, growth deceleration and diminution probably happened here in because calli started to exhaust nutrients of the medium; growth reduction due to cell signaling mechanism ; or cell death due to decrease in nutrient availability, smaller physical area and air available for growth inside the vessel as well as gases exchanges between cells and media inside the vessel's environment (Pépin et al, 2004). The stresses faced by cells in culture may include the cell growth rate, the maximum cell mass to a certain physical space and the size distribution of cell aggregates (Tanaka et al, 1988).…”

Section: Growth Curve Evaluationmentioning

confidence: 99%

Calogênese, curva de crescimento de calos e formação de suspensões celulares de Lavandula

Bona

Santos²,

Biasi

2012

Agraria

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The cultivation of calli and cell suspension lines of aromatic plants is important because they are considered to be factories of active compounds such as, for instance, the rosmarinic acid found in Lavandula species. L. angustifolia is considered the most important species because it is regarded to be practically free from camphor, differently from other species in the genus, however, it is less productive than L. dentata, which is very productive and easily asexually propagated, but presents higher content of camphor. Information about calli and cell suspensions induction and maintenance for Lavandula species, especially the latter, is scarce. Not only that, but once callus formation is induced, it is necessary to identify the growth dynamic of the cells in order to maintain healthy, logarithmically phased growing cells for future elicitation. The goals of this work were to induce calli formation, evaluate growth curve and produce cell suspensions of Lavandula angustifolia and Lavandula dentata, two Lavandula species of great economical importance. Apical and lateral buds of L. angustifolia and L. dentata were placed on MS + 1 mg.L -1 2.4-D or picloran + 0.5 mg.L -1 BAP or 1 mg.L -1 2.4-D + 0.5 mg.L -1 GA 3 , in the presence of light or in the dark. Calli were obtained only from L. angustifolia, under light, with the combination of 2.4-D + GA 3 . The growth curve of L. angusifolia calli indicated the need for media exchange every three weeks. A blue pigment which may be interesting as a dye in the food industry was present in the media. Cells suspensions were formed.Key words: Growth regulator, lavender, micropropagation, tissue culture. Calogênese, curva de crescimento de calos e formação de suspensões celulares de Lavandula RESUMO O cultivo de calos e suspensões celulares de plantas aromáticas é importante porque estes são considerados fábricas de compostos ativos como, por exemplo, o ácido rosmarínico encontrado em espécies de Lavandula. L. angustifolia é considerada a espécie mais importante por ser praticamente livre de cânfora, todavia, é menos produtiva que L. dentata, que é facilmente propagada vegetativamente, mas apresenta alto teor de cânfora. Informação sobre indução e manutenção de calos e suspensões celulares de espécies de Lavandula, especialmente a última, é escassa. Não somente isto, mas uma vez que a formação de calos é induzida, é necessário identificar o crescimento dinâmico das células para mantê-las com crescimento saudável, em fase logarítmica para futuras elicitações. Os alvos deste trabalho foram indução de calos, avaliação de curva de crescimento e formação de suspensões celulares de Lavandula angustifolia e Lavandula dentata, duas espécies de Lavandula de grande importância econômica. Gemas de L. angustifolia e L. dentata foram colocadas em MS + 1 mg.L -1 2,4-D ou picloran + 0,5 mg.L -1 BAP ou 1 mg.L -1 2,4-D + 0,5 mg.L -1 GA 3 , em condições de luz, ou no escuro. Foram obtidos calos de L. angustifolia em condições de luz, sendo 2,4-D + GA 3 a melhor combinação. A curva de cr...

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“…그리고 한 세포응집체 내에 세포 간의 영양분 및 산소구배가 형성되며 불균등한 환경이 형성되기 쉽다 [10]. 또한 세포응집체의 표면에 있는 세포는 전단응력에 비교적 큰 손상을 입으므로 세포주에 적합한 효율적인 교반전략과 최적화가 요구된다 [11]. 조정배지는 세포를 회수하고 남은 배양액으로 세포로부터 분비된 글루코오스, 아미노산, 핵산과 같은 대사산물, 인터루 킨, EGF (epidermal growth factor)와 같은 성장인자 그리고 세 포 외 기질 단백질 등을 포함하고 있다 [12,13].…”

Section: Introductionunclassified

Effects of Mixing Performance and Conditioned Medium on hCTLA4Ig Production in Transgenic Rice Cell Suspension Cultures

최홍열¹,

박준용²,

남형진³

et al. 2015

KSBB Journal

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Transgenic rice cells using RAmy3D promoter can provide high productivity, and the production of recombinant protein is induced by sugar starvation. In this system, productivity was reduced during the scale-up processes. To ensure the influences of shear stress and oxygen transfer rate, working volume and mixing performances were investigated under various agitation speeds and working volumes. In addition, inoculation methods including suspended cells and filtered cells were compared. Working volumes and shaking speeds were 300, 450 mL and 80, 120 rpm, respectively. Hydrodynamic environment of each condition was measured numerically like mixing time and k L a. Good mixing performance and high shear stress were measured at high agitation speed and low volume. The highest level of hCTLA4Ig was 30.7 mg/L at 120 rpm, 300 mL. When conditioned medium was used for inoculation, increased cell growth was noticed during the day 0~4 and decreased slower than filtered cells. Compared with filtered cells, the maximum hCTLA4Ig level reached 37.8 mg/L at 120 rpm, 300 mL and lower protease activity level was observed. In conclusion mixing performance is critical factor for productivity and conditioned medium can have a positive effect on damaged cells caused by hydrodynamic shear stress.

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The effect of physical stress on plant cells in suspension cultures

Cited by 53 publications

References 6 publications

The effect of inoculum density and conditioned medium on the production of ajmalicine and catharanthine from immobilizedCatharanthus roseus cells

The effect of inoculum density and conditioned medium on the production of ajmalicine and catharanthine from immobilizedCatharanthus roseus cells

Calogênese, curva de crescimento de calos e formação de suspensões celulares de Lavandula

Effects of Mixing Performance and Conditioned Medium on hCTLA4Ig Production in Transgenic Rice Cell Suspension Cultures

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