The effect of phosphate starvation on the lipid and fatty acid composition of the fresh water eustigmatophyte Monodus subterraneus

Khozin‐Goldberg, Inna; Cohen, Zvi

doi:10.1016/j.phytochem.2006.01.010

Cited by 395 publications

(208 citation statements)

References 29 publications

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“…4C) was reduced under phosphate-limiting conditions. It has been also showed that the cellular lipid content of starved cells in Monodus subterraneous was increased due to the increase in the TAGs levels which at the same time decreased the production of phospholipids (Khozin-Goldberg and Cohen, 2006). This is consistent with the findings of Fidalgo and co-workers (1998), who stated that microalgae produce higher unsaturated fatty acid levels during stationary growth phase, where the nutrient concentration is limited.…”

Section: Discussionsupporting

confidence: 87%

“…It is also well established that algal growth rate depends on the phosphorus levels in cells (Larsdotter, 2006) and microalgae species. Variations in phosphate concentrations, described by Khozin-Goldberg and Cohen (2006), affected the accumulation of fatty acid and total oil production in Monodus subterraneus. However, the literature on the effect of phosphate on gene expression engaged in fatty acid biosynthesis in microalgae is limited.…”

Section: Introductionmentioning

confidence: 99%

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Phosphate-induced changes in fatty acid biosynthesis in Chlorella sp. KS-MA2 strain

Aziz¹,

Osman²,

San³

et al. 2016

bta

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Phosphate is involved in fatty acid biosynthesis in microalgae cells. The influence of phosphate concentrations on polyunsaturated fatty acids' profile, elongation, and desaturation of fatty acids chains during their biosynthesis still remains unclear. Protein ketoacyl-ACP synthase-I (KAS-1 ) is required for the addition of malonyl-CoA for the elongation of butyryl-ACP from a 4-to 14-carbon chain. The omega-6 (ω-6 FAD ) and omega-3 desaturases (ω-3 FAD ) are involved in the insertion of double bonds into the pre-formed fatty acid chains. This study reports the effect of phosphate concentrations on the growth, fatty acid content, and level of KAS-1, ω-6 FAD, and ω-3 FAD transcripts in Chlorella KS-MA2 strain at the stationary growth phase. Phosphate was tested at concentrations 9, 18, 36, and 72 μM and the fatty acid content was analyzed using gas chromatography. Gene expression levels were measured using quantitative real-time-PCR. Results showed that cell growth, amount of saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFAs) did not significantly (P > 0.05) differ among tested treatments. The highest concentration of α-linolenic acid and the expression level of ω-3 FAD were recorded with 72 μM of phosphate in culture medium. The KAS-1 and ω-6 FAD transcripts levels decreased when the concentration of phosphate was below 36 μM. Phosphate in F/2 medium was sufficient to enhance the biosynthesis of major fatty acids in Chlorella sp.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Phosphate-induced changes in fatty acid biosynthesis in Chlorella sp. KS-MA2 strain

Aziz¹,

Osman²,

San³

et al. 2016

bta

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“…There are reports that amount of MUFAs goes up due to the reduction of nitrogen in the culture medium 20,23 . The potential relationship between phosphorus and amount of MUFAs have beenshown intwo microalgae Phaeodactylum tricornutum and Dunaliella tertiolecta 48 and the yellow-green alga, Monodus subterraneus 49 , in which reduced phosphorus in medium had impacted MUFAs. By analyzing the changes in fatty acids composition of several algal species at different concentrations of nitrogen and silica, Shifrin and Chisholm 50 showed increase in MUFAs as the result of nitrogen limitation.…”

Section: Discussionmentioning

confidence: 99%

Effect of nutrients on total lipid content and fatty acids profile of Scenedesmus obliquus

Darki

Seyfabadi

Fayazi

2017

Braz. arch. biol. technol.

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“…Phosphorus limitation resulted in increased lipid content, mainly TAG, in Monodus subterraneus (Eustigmatophyceae) (Khozin-Goldberg and Cohen, 2006), P. tricornutum and Chaetoceros sp. (Bacillariophyceae), and I. galbana and Pavlova lutheri (Prymnesiophyceae), but decreased lipid content in Nannochloris atomus (Chlorophyceae) and Tetraselmis sp.…”

Section: Nutrientsmentioning

confidence: 99%

Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances

et al. 2008

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SummaryMicroalgae represent an exceptionally diverse but highly specialized group of micro-organisms adapted to various ecological habitats. Many microalgae have the ability to produce substantial amounts (e.g. 20-50% dry cell weight) of triacylglycerols (TAG) as a storage lipid under photo-oxidative stress or other adverse environmental conditions. Fatty acids, the building blocks for TAGs and all other cellular lipids, are synthesized in the chloroplast using a single set of enzymes, of which acetyl CoA carboxylase (ACCase) is key in regulating fatty acid synthesis rates. However, the expression of genes involved in fatty acid synthesis is poorly understood in microalgae. Synthesis and sequestration of TAG into cytosolic lipid bodies appear to be a protective mechanism by which algal cells cope with stress conditions, but little is known about regulation of TAG formation at the molecular and cellular level. While the concept of using microalgae as an alternative and renewable source of lipid-rich biomass feedstock for biofuels has been explored over the past few decades, a scalable, commercially viable system has yet to emerge. Today, the production of algal oil is primarily confined to high-value specialty oils with nutritional value, rather than commodity oils for biofuel. This review provides a brief summary of the current knowledge on oleaginous algae and their fatty acid and TAG biosynthesis, algal model systems and genomic approaches to a better understanding of TAG production, and a historical perspective and path forward for microalgae-based biofuel research and commercialization.

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The effect of phosphate starvation on the lipid and fatty acid composition of the fresh water eustigmatophyte Monodus subterraneus

Cited by 395 publications

References 29 publications

Phosphate-induced changes in fatty acid biosynthesis in Chlorella sp. KS-MA2 strain

Phosphate-induced changes in fatty acid biosynthesis in Chlorella sp. KS-MA2 strain

Effect of nutrients on total lipid content and fatty acids profile of Scenedesmus obliquus

Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances

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