The Effect of pH and Color Stability of Anthocyanin on Food Colorant

Wahyuningsih, Sayekti; Wulandari, Lilis; Wartono, Muhammad Widyo; Munawaroh, H; Ramelan, Ari Handono

doi:10.1088/1757-899x/193/1/012047

Cited by 125 publications

(73 citation statements)

References 9 publications

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“…The maximum peaks of absorbance of the fungal extracts subjected to the pH gradient were obtained as follows: red-pink coloration at pH 9-10 (λ max1 = 300 nm and λ max2 = 500 nm); purple coloration found at pH 12-13 (λ max1 = 290 nm and λ max2 = 555 nm), and blue coloration found at pH 14 (λ max1 = 295 nm and λ max2 = 580 nm). These changes of color in the presence of different pH solutions corroborated the presence of phenolic-amines or anthocyanin-like compounds [51]. Absorbance measurements indicated that the strain Neocosmospora sp.…”

Section: Metabolite Profiling Of the H17 Strain With Or Without Leadsupporting

confidence: 60%

Characterization of Fungal Endophytes Isolated from the Metal Hyperaccumulator Plant Vachellia farnesiana Growing in Mine Tailings

et al. 2020

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Heavy metal pollution has become an environmental and health problem worldwide. With the aim of finding novel strategies for metal bioremediation, endophytic fungi from the heavy metal hyperaccumulator plant Vachellia farnesiana were isolated and characterized. The plants were growing in mine tailings, rich in Zn, Pb, and Cu. Morphological and phylogenetic analyses indicated that the fungal strains belonged to Neocosmospora and Aspergillus genera. The Neocosmospora isolate belongs to the Fusarium solani species complex (FSSC) that groups phytopathogen species. However, in this case the plants from which it was isolated did not show any signs of disease. Both fungal strains were able to remove significant amounts of heavy metals from liquid cultures, either in a mixture of the three metals or each metal in a single culture. In response to lead exposure, the Neocosmospora sp. strain secreted specific novel phenolic compounds other than anthraquinones or naphtoquinones, which have been described in similar situations. The Aspergillus sp. dropped the pH in the medium. High-performance liquid chromatography determinations indicated that this strain secreted mainly glutamic acid in response to lead, a novel mechanism, which has not been reported elsewhere. Malic and succinic acids were also produced in response to lead exposure. Possibly, glutamic and succinic acids (synthesized in the Krebs cycle) can be used to cope with metal toxicity due to the plant providing photosynthates to the fungus. These fungi showed the potential to be used for bioremediation or restoration of metal-polluted environments.

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Section: Metabolite Profiling Of the H17 Strain With Or Without Leadsupporting

confidence: 60%

Characterization of Fungal Endophytes Isolated from the Metal Hyperaccumulator Plant Vachellia farnesiana Growing in Mine Tailings

et al. 2020

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“…Absolute methanol (200 mL) was then added to the fine powder (2 gm) and the solution was transferred to the soxhlet apparatus for the extraction of phytochemicals (Dhawan and Gupta, 2017). The obtained leaf extract (LE) was first filtered and the filtrate was concentrated in the rotary evaporatory (Wahyuningsih et al, 2017). Moreover, root exudates (RE) was obtained from filtered hypdroponic culture of the root seedlings and concentrated in the rotary evaporatory.…”

Section: Methodsmentioning

confidence: 99%

Phytohormonal cross-talk modulate Bipolaris sorokiniana (Scc.)interaction with Zea mays

Yousaf

Hussain

Hamayun

et al. 2019

Preprint

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16Besides acting as growth inducing molecule, Gibberellin (GA 3 ) also confers the compatibility 17 of microbial interactions with host. We inoculated 11 days old Z. mays seedlings grown under 18 hydroponic conditions and high GA 3 levels with Bipolaris sorokiniana (BIPOL) at the spore 19 density (SD) of OD 0.6 . The high level of GA 3 negatively affected the growth of the seedlings, 20 accompanied by the high level of stress deducing secondary metabolites (proline, total 21 flavanoids, phenylpropanoids, and glucosinolides). Moreover, high level of GA 3 produced a 22 hypersensitive response (HR) in the seedlings. The HR developed cross talks with IAA and 23 trans-zeatins and triggered higher production of hypersensitive inducing biomolecules. The 24 other HR co-related biological processes were demonstrated by high phytoalexins level and 25 high protease activities. Such activities ultimately inhibited the colonization of BIPOL on the 26 roots of maize seedlings. The products of the genes expressed at high GA 3 also conferred the 27 deterrence of BIPOL colonization at SD = OD 0.6 . Intriguingly, when we inhibited GA 3 28 biosynthesis in the seedlings with aerially sprayed uniconizole, prior to BIPOL treatment, the 29 BIPOL colonized and subsequently promoted the seedling growth. This low level of GA 3 30 after BIPOL treatment checked the high level of secondary metabolites and hypersensitivity 31 inducing molecules. The results, thus suggested that the aforementioned processes only 32 happened in the BIPOL at SD (OD 0.6 ), whereas the SD at lower levels (OD 0.2 or OD 0.4 ) 33 neither promoted the growth of uniconizole pre-treated seedlings nor produced HR in control 34 seedlings of maize plant.35

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“…Color of CMC-based films changed from violet to pink for BP extracts and from red to pink for RP extract. At lower acidic pH, reddish taint of anthocyanins was dominant and as the pH increased the molecules become deprotonated (Wahyuningsih, Wulandari, Wartono, Munawaroh, & Ramelan, 2017) and changed to less intensive rose/pink taint. This change was less evident in CS films.…”

Section: Evaluation Of Films As Ph Indicators-relationship Between Chmentioning

confidence: 99%

Comparison of Two pH Responsive Color Changing Bio‐Based Films Containing Wasted Fruit Pomace as a Source of Colorants

Kurek

Hlupić

Ščetar

et al. 2019

Journal of Food Science

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The main objective of this work is to develop and characterize novel bio‐based sensor as intelligent food packaging film to monitor quality changes in fresh chicken meat. Chitosan (CS) and carboxymethyl cellulose (CMC) were used as polymer matrices for immobilization of pH sensitive color indicators. Generally considered as a food waste, blueberry (BP) and red grape skin pomace (RP) extracts were used as indicators at total phenolic content (TPC) of 0.06, 0.13, and 0.25 w/v for BP, and 0.006, 0.012, and 0.02 w/v for RP‐based films. Color, mechanical, and intelligent material properties were determined. CS films were more elastic than CMC, while no significant changes in mechanical properties occurred after the addition of both extracts at all concentrations. Notable and eye recognizable color changes in dry films were seen. It was the most significant in the pH range from 4 to 7. For CMC‐based films, color changed from violet to pink for BP extracts and from red to pink for RP extracts. For CS films, the changes were less significant. The color taint and intensity changed toward green in blueberry‐based films and to orange in grape seed extract‐based films. Significant color changes were observed after 46 hr in application tests on fresh chicken meat. This was correlated to pH changes of meat above 6 that was one of the parameters considered as an indication of spoilage. Practical Application This work provides interesting data to food industry. It offers an idea and possibility of managing food waste from fruits and vegetables industry that makes problems and costs when needed to be disposed in a proper manner. Moreover, there is the possibility of producing novel kind of food packaging materials that could be used in order to check the end of food shelf‐life in an inexpensive and natural way. Additional value lies in the fact that materials and procedures could be considered as sustainable.

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The Effect of pH and Color Stability of Anthocyanin on Food Colorant

Cited by 125 publications

References 9 publications

Characterization of Fungal Endophytes Isolated from the Metal Hyperaccumulator Plant Vachellia farnesiana Growing in Mine Tailings

Characterization of Fungal Endophytes Isolated from the Metal Hyperaccumulator Plant Vachellia farnesiana Growing in Mine Tailings

Phytohormonal cross-talk modulate Bipolaris sorokiniana (Scc.)interaction with Zea mays

Comparison of Two pH Responsive Color Changing Bio‐Based Films Containing Wasted Fruit Pomace as a Source of Colorants

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