The effect of paromomycin and [psi] on the suppression of mitochondrial mutations in Saccharomyces cerevisiae

Dujardin, Geneviève; Lund, Patricia; Słonimski, Piotr P.

doi:10.1007/bf00396200

Cited by 11 publications

(2 citation statements)

References 53 publications

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“…To confirm that the appearance of V2p is really the result of an alteration in translation, we assessed whether its synthesis is affected by paromomycin, an antibiotic that changes translation fidelity of cytosolic as well as of mitochondrial ribosomes (Wilhelm et al, 1978;Dujardin et al, 1984;Zagorski et al, 1987a). Translation in the presence of Na 2 [ 35 S]O 4 was monitored in mitochondria incubated either with or without paromomycin (see Materials and Methods) after isolation from the wild type, mitas well as from the mrf1-136 and mrf1-145 cells.…”

Section: Changes In the Fidelity Of Mitochondrial Translation Results mentioning

confidence: 99%

Translational readthrough of a termination codon in the yeast mitochondrial mRNA VAR1 as a result of mutation in the release factor mRF1.

Claisse

Boguta²,

Zagórski³

2005

Acta Biochim Pol

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Yeast mitochondrial DNA codes for eight major polypeptides. Translation of he mitochondrially encoded polypeptides in strains with mutated mitochondrial release factor, mRF1, was found to result in the synthesis of a novel protein, V2. Different mrf1 alleles were associated with different efficiency of V2p synthesis. Translation of V2p was enhanced by paromomycin. Comparative analysis of peptides resulting from protease digestion indicated that V2p is a derivative of Var1p. According to our hypothesis, V2p represents a readthrough product of the natural stop codon in VAR1 mRNA.An effective way of investigating the expression of genes in mtDNA is based on studying the control exerted by the nucleus over mitochondrial functions. This is due to replication, transcription and translation apparatus in mitochondria being composed mostly of polypeptides encoded by nuclear DNA. The yeast Saccharomyces cerevisiae, being a facultative anaerobe, offers distinct advantages for mitochondrial genetic studies. Several hundred respiratory deficient point mutations or small deletions, called mit -, have been genetically mapped and assigned to genes in mtDNA. Respiration of mitstrain Vol. 52 No. 1/2005 [129][130][131][132][133][134][135][136][137] QUARTERLY .

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Section: Changes In the Fidelity Of Mitochondrial Translation Results mentioning

confidence: 99%

Translational readthrough of a termination codon in the yeast mitochondrial mRNA VAR1 as a result of mutation in the release factor mRF1.

Claisse

Boguta²,

Zagórski³

2005

Acta Biochim Pol

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“…Using antibodies raised against the b14 maturase Jacq et al (1984) have shown that this maturase can only be detected in mutant strains that overproduce the maturase. Dujardin et al (1984) were able to show that paromomycin preferentially suppresses nonsense mutations in mRNA maturases and Weiss-Brummer et al (1984) have demonstrated that frame-shift mutations in the upstream exons of the cob-box gene are sufficiently leaky to allow the processing of the oxi3 transcript, which implies that mRNA maturases are synthesized. All these data support the idea that only a small amount of maturase is needed for splicing.…”

Section: Discussionmentioning

confidence: 99%

The NAM2 proteins from S. cerevisiae and S. douglasii are mitochondrial leucyl-tRNA synthetases, and are involved in mRNA splicing.

et al. 1988

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We have cloned and sequenced the NAM2 gene of Saccharomyces douglasii, which is a homologue of the NAM2 gene of Saccharomyces cerevisiae. The wild‐type S.douglasii gene possesses the suppressor functions of the mutant S. cerevisiae NAM2‐1 allele, being able to cure a mitochondrial b14 maturase deficiency. By sequence comparisons and direct measurements we have demonstrated that the NAM2 genes encode mitochondrial leucyl tRNA synthetases (EC 6.1.1.4.). Using a derivative of the NAM2 gene, where the expression of the gene is under the control of the UAS GAL10, we have shown that the processing of the pre‐mRNA from the two mosaic genes oxi3 and cob‐box is impaired when transcription of the gene is repressed. These results lead us to conclude that the mitochondrial leucyl tRNA synthetase is involved in protein synthesis and mRNA splicing. Sequence comparisons show that the mitochondrial and Escherichia coli leucyl tRNA synthetases are highly homologous; however, significant features which may be important for the splicing functions of the mitochondrial enzymes are absent from the bacterial enzyme.

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Mim3 and nam3 Omnipotent Suppressor Genes of the Yeast S. Cerevisiae Affect the Polypeptide Composition of the 37s Mitoribosomal Subunit.

Zagórski¹,

Mieszczak²,

Boguta³

et al. 1988

Genetics of Translation

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The effect of paromomycin and [psi] on the suppression of mitochondrial mutations in Saccharomyces cerevisiae

Cited by 11 publications

References 53 publications

Translational readthrough of a termination codon in the yeast mitochondrial mRNA VAR1 as a result of mutation in the release factor mRF1.

Translational readthrough of a termination codon in the yeast mitochondrial mRNA VAR1 as a result of mutation in the release factor mRF1.

The NAM2 proteins from S. cerevisiae and S. douglasii are mitochondrial leucyl-tRNA synthetases, and are involved in mRNA splicing.

Mim3 and nam3 Omnipotent Suppressor Genes of the Yeast S. Cerevisiae Affect the Polypeptide Composition of the 37s Mitoribosomal Subunit.

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