The effect of papaya leaf extract (Carica papaya l.) to the bleeding time on mice with trombositopenia

Abstract

<p align="center"><strong>ABSTRAK</strong></p><p><strong>Latar belakang: </strong>Trombositopenia merupakan suatu keadaan jumlah trombosit di bawah 150.000/mm<sup>3</sup>, hal ini dapat menyebabkan perdarahan yang apabila tidak ditangani dengan baik dapat berujung pada kematian. Beberapa tindakan yang dapat dilakukan untuk mengatasi trombositopenia, salah satunya adalah dengan tanaman daun pepaya.</p><p><strong>Tujuan:</strong> untuk menganalisis pengaruh pemberian ekstrak daun pepaya terhadap <em>bleeding time</em> (waktu perdarahan).</p><p><strong>Metode : </strong>Desain penelitian ini menggunakan <em>true experimental</em> dengan rancangan <em>post test only control group design</em>. Sampel dalam penelitian ini sejumlah 30 ekor mencit jantan (<em>Mus musculus</em>) yang dibagi menjadi lima kelompok. Kelompok pertama sebagai kelompok kontrol negatif (CMC Na 0,5%), kelompok kedua sebagai kontrol positif (CMC Na 0,5%)<a href="file:///D:/peer/VOLUME%206/VOLUME%206%20NOMOR%203/KIRIM%20LINTANG%20VOLUME%206%20ISSUE%203,%202018%20(AKREDITASI)/6.%20Revisi%20Publish%20JGDI%20(1)_Yudha.rtf#_msocom_1">[a1]</a> <a href="file:///D:/peer/VOLUME%206/VOLUME%206%20NOMOR%203/KIRIM%20LINTANG%20VOLUME%206%20ISSUE%203,%202018%20(AKREDITASI)/6.%20Revisi%20Publish%20JGDI%20(1)_Yudha.rtf#_msocom_2">[A2]</a> , kelompok ketiga diberi esktrak etanol 96% daun pepaya dosis 0,5g/kgBB, kelompok keempat diberi esktrak etanol 96% daun pepaya dosis 1g/kgBB, kelompok kelima diberi esktrak etanol 96% daun pepaya dosis 2g/kgBB. Pengumpulan data menggunakan lembar pengukuran <em>bleeding time</em>, yang dianalisa dengan uji ANOVA.</p><p><strong>Hasil: </strong>Hasil penelitian ini menunjukkan bahwa didapatkan nilai <em>p</em><0.0001 dengan rerata waktu paling cepat antara kelompok perlakuan yaitu kelompok perlakuan dengan dosis 2g/kgBB (2.74±0.14). Hal ini menunjukkan bahwa terdapat perbedaan signifikan antar semua kelompok. <a href="file:///D:/peer/VOLUME%206/VOLUME%206%20NOMOR%203/KIRIM%20LINTANG%20VOLUME%206%20ISSUE%203,%202018%20(AKREDITASI)/6.%20Revisi%20Publish%20JGDI%20(1)_Yudha.rtf#_msocom_3">[a3]</a> <a href="file:///D:/peer/VOLUME%206/VOLUME%206%20NOMOR%203/KIRIM%20LINTANG%20VOLUME%206%20ISSUE%203,%202018%20(AKREDITASI)/6.%20Revisi%20Publish%20JGDI%20(1)_Yudha.rtf#_msocom_4">[A4]</a> </p><p><strong>Kesimpulan: </strong>Kesimpulan dari penelitian ini adalah pemberian ekstrak daun pepaya (<em>Carica papaya L.</em>) dapat mempercepat <em>bleeding time</em> (waktu perdarahan).</p><p><strong>KATA KUNCI: </strong>daun pepaya; <em>bleeding time, </em>trombositopenia<strong></strong></p><p align="center"> </p><p align="center"><strong>ABSTRACT</strong></p><p><strong><em>Background</em></strong><strong>: </strong>Thrombocytopenia is a condition of platelet counts below 150,000/mm<sup>3</sup>, this may cause bleeding which, if not handled properly, may lead to death. Some actions that can be done to overcome thrombocytopenia, one of which is with papaya leaf.</p><p><strong>Objectives:</strong> This study aims to analyze the effect of giving papaya leaf extract to bleeding time.</p><p><strong><em>Methods:</em></strong><strong> </strong>This research design uses true experimental with design of post-test only control group design. The sample in this study were 30 male mice (<em>Mus musculus</em>) divided into five groups. The first group as the negative control group (CMC Na 0.5%), the second group as the positive control (CMC Na 0.5%), the third group was given ethanol 96% extract papaya leaf dose 0.5g/kgBW, the fourth group was given ethanol 96% extract papaya leaf dose 1g/kgBW, the fifth group was given ethanol 96% extract papaya leaf dose 2g/kgBW. Data collection using measurement sheet of bleeding time which were analyzed by ANOVA test.</p><p><strong><em>Results:</em></strong><strong> </strong>The results of this study showed that p<0.0001 the fastest mean time occur between treatment groups that is treatment group with dose 2g/kgBW (2.74±0.14). In the bleeding time variables p<0.0001 the fastest mean time occur between treatment groups that is treatment group with dose 2g/kgBW (2.74±0.14). This result shows that there are significant differences between every groups.</p><p><strong><em>Conclusion:</em></strong><strong> </strong>The conclusion of this research is giving papaya leaf extract can increase bleeding time.</p><p> </p><p><strong>KEYWORDS: </strong>leaf of papaya, bleeding time, thrombocytopenia.</p><p> </p>