The effect of ovariectomy on bone metabolism in rats

N, Omi; Ezawa, Ikuko

doi:10.1016/8756-3282(95)00329-c

Cited by 84 publications

(74 citation statements)

References 9 publications

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“…Although the administration of APS increased the uterine weight compared to that of the OVX group, it was still lower than that of the Sham group. Furthermore, a previous study showed that the body weight gain in OVX rats increased significantly compared with that in the Sham rats (Omi and Ezawa, 1995). In line with this study, we found that the OVX group showed a significant increase in body weight gain than the Sham group did.…”

Section: Discussionsupporting

confidence: 81%

Effect of Astragalus polysaccharides on ovariectomy-induced osteoporosis in mice

Huo¹,

Sun²

2016

Genet. Mol. Res.

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ABSTRACT. Postmenopausal osteoporosis, a common type of osteoporosis in women, has become a serious public health issue. Astragalus polysaccharides (APS), possessing various pharmacological activities, are the active ingredients of Radix Astragali. It can be advantageous in the treatment of postmenopausal osteoporosis. In the present study, we evaluated the potential therapeutic effects of APS on postmenopausal osteoporosis by using a mice model induced by ovariectomy (OVX). Forty-eight female 6-week-old outbred ICR mice were randomly divided into six groups (N = 8): Sham group, OVX group, 17 b-estradiol (E2, 0.1 mg/kg)-treated OVX group, and APS (at three doses: 100, 200, and 400 mg/kg)-treated OVX groups. The effect of APS on the bone mineral density (BMD) was determined using dualenergy X-ray absorptiometry. The serum levels of receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG), osteocalcin, and tumor necrosis factor (TNF)-a were measured using ELISA kits. The results revealed that APS exerted significant antiosteoporotic activity by increasing the BMD considerably in a dosedependent manner. APS treatment reduced the serum RANKL levels considerably and increased the serum OPG levels, thereby lowering the ratio of RANKL/OPG. Furthermore, APS also markedly reduced osteocalcin and TNF-a concentration in OVX-induced postmenopausal osteoporosis mice model. These results showed that APS exerts a protective effect on bone loss in OVX mice. The molecular mechanism underlying this effect be the reduction of bone resorption and inhibition of osteoclastogenesis. Our findings suggest that APS may be a potential strategy for the prevention and treatment of postmenopausal osteoporosis.

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Section: Discussionsupporting

confidence: 81%

Effect of Astragalus polysaccharides on ovariectomy-induced osteoporosis in mice

Huo¹,

Sun²

2016

Genet. Mol. Res.

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“…The LF treatment did not cause differentiation of the bone marrow stromal cells to an osteoblastic lineage in this ovariectomized rat model. According to previous studies [3] , however, the inhibition of bone resorption markers by oral LF suggests that LF may prevent Ovx-induced bone loss via a preferential anti-osteoclastogenic pathway.…”

Section: Discussionmentioning

confidence: 99%

“…The bone loss is caused by an imbalance between the activities of osteoclasts and osteoblasts, and it leads to the uncoupling of bone resorption and bone formation [2] . The bone loss found in postmenopausal osteoporosis (PMOP) cases is caused by estrogen deficiency that increases osteoclast activity and bone remodeling -a predominance of bone resorption over bone formation [3] . The increased rate of bone turnover as observed from markers, such as β-CrossLaps (β-CTx) and collagen type I N-telopeptide (NTX), may be an additional risk factor for fractures by exacerbating bone loss.…”

Section: Introductionmentioning

confidence: 99%

Bovine lactoferrin improves bone mass and microstructure in ovariectomized rats via OPG/RANKL/RANK pathway

Hou

Xue

2012

Acta Pharmacol Sin

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Aim: Lactoferrin (LF), an 80-kDa iron-binding glycoprotein, is a pleiotropic factor found in colostrum, milk, saliva and epithelial cells of the exocrine glands. The aim of this study was to evaluate the effects of LF on the bones in ovariectomized (Ovx) rats and to identify the pathways that mediate the anabolic action of LF on the bones. Methods: Female Sprague-Dawley rats (6-month-old) underwent ovariectomy, and were treated with different doses of LF (10, 100, 1000, and 2000 mg·kg -1 ·d -1 , po) or with 7β-estradiol (0.1 mg·kg -1 , im, each week) as the positive control. By the end of 6 month-treatments, the bone mass and microstructure in the rats were scanned by micro-computed tomography (micro-CT), and the bone metabolism was evaluated with specific markers, and the mRNA levels of osteoprotegerin (OPG) and the receptor-activator of nuclear factor κB ligand (RANKL) in femur were measured using qRT-PCR. Results: LF treatment dose-dependently elevated the bone volume (BV/TV), trabecular thickness (TbTh) and trabecular number (TbN), and reduced the trabecular separation (TbSp) in Ovx rats. Furthermore, higher doses of LF (1000 and 2000 mg·kg -1 ·d -1 ) significantly increased the bone mineral density (BMD) compared with the untreated Ovx rats. The higher doses of LF also significantly increased the serum levels of OC and BALP, and decreased the serum levels of β-CTx and NTX. LF treatment significantly increased the OPG mRNA levels, and suppressed the RANKL mRNA levels, and the RANKL/OPG mRNA ratio in Ovx rats. Conclusion: Oral administration of LF preserves the bone mass and improves the bone microarchitecture. LF enhances bone formation, reduces bone resorption, and decreases bone mass loss, possibly through the regulation of OPG/RANKL/RANK pathway.

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“…A recent study by Fujimura et al 22 has been reported that the local administration of alendronate sodium hydrate has inhibited orthodontic tooth movement and root resorption in male mice. Classically, ovariectomized rats have been advocated as a good osteoporotic animal model 23 and often have been used to study efficacy of drugs aimed to prevent bone loss. 16,[24][25][26][27][28] Due to the similarity among of the morphology and function of the cells, in both, root resorption and bone resorption processes could be considered similar.…”

Section: Introductionmentioning

confidence: 99%

Orthodontic tooth movement and root resorption in ovariectomized rats treated by systemic administration of zoledronic acid

Sirisoontorn

Hotokezaka

Hashimoto

et al. 2012

American Journal of Orthodontics and Dentofacial Orthopedics

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The effect of ovariectomy on bone metabolism in rats

Cited by 84 publications

References 9 publications

Effect of Astragalus polysaccharides on ovariectomy-induced osteoporosis in mice

Effect of Astragalus polysaccharides on ovariectomy-induced osteoporosis in mice

Bovine lactoferrin improves bone mass and microstructure in ovariectomized rats via OPG/RANKL/RANK pathway

Orthodontic tooth movement and root resorption in ovariectomized rats treated by systemic administration of zoledronic acid

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