The Effect of Ouabain and Ethacrynic Acid on ATPase Activities in the Inner Ear of the Rat and Guinea Pig

Abstract

The effect of ethacrynic acid on ATPase activities in the stria vascularis of rat and guinea pig inner ear was studied. Both Na-K- and Mg-activated ATPase activities were inhibited non-selectively only at very high concentrations of ethacrynic acid, in contrast to the selective inhibition of Na-K-ATPase activity by ouabain. It is suggested that the deleterious effect of ethacrynic acid on the inner ear may be due to its effect on the general metabolism of the cell rather than on the ionic transport system.

Contributions of Mouse and Human Hematopoietic Cells to Remodeling of the Adult Auditory Nerve After Neuron Loss

“…It has been used as a blocking agent to study the functional role of Na,K-ATPase in inner ear fluid and ion homeostasis. 18,19,20 Application of ouabain to the round window of gerbils and mice results in a rapid and highly selective elimination of type I SGNs without degeneration of cells within the organ of Corti, the strial vascularis and spiral ligament in the cochlear lateral wall. 3,21,22 Here, we used this approach to investigate the effects of acute injury on tissue engraftment of mouse and human hematopoietic cells in the adult AN using a mouse-mouse bone marrow transplantation model and a human-mouse hematopoietic cell transplantation model.…”