The effect of organic ligand modification on protein corona formation of nanoscale metal organic frameworks

Wang, Wenhao; Liu, Huihui; Huang, Zhengwei; Fu, Fangqin; Wang, Wenhua; Wu, Lu; Huang, Ying; Wu, Chuanbin; Pan, Xin

doi:10.1016/j.cclet.2022.02.052

Cited by 14 publications

(4 citation statements)

References 29 publications

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“…The protein amount in the lysate was determined with a BCA assay. The required amount of protein was loaded onto precast 10% SDS-PAGE gels . After electrophoresis, the gel was stained with Coomassie brilliant blue and imaged with Molecular Imager ChemiDoc XRS (Bio-Rad Laboratories, Hercules, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…The required amount of protein was loaded onto precast 10% SDS-PAGE gels. 32 After electrophoresis, the gel was stained with Coomassie brilliant blue and imaged with Molecular Imager ChemiDoc XRS (Bio-Rad Laboratories, Hercules, CA, USA). In addition, the digested peptides were extracted and analyzed by nano-liquid chromatography−mass spectrometry (nano-LC−MS/MS).…”

Section: Cellmentioning

confidence: 99%

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Glycyrrhizic Acid-Lipid Framework Nanovehicle Loading Triptolide for Combined Immunochemotherapy

Xu,

Huang,

et al. 2023

ACS Appl. Mater. Interfaces

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Despite the acknowledged advantages of combined immunochemotherapy for tumor treatment, the high efficiency of co-delivery of these combined agents into the targeted tumor tissue is still challenging. Herein, based on a "three-birds-with-one-stone" strategy, a facile glycyrrhizic acid (GL)-lipid hybrid nanoplatform loading triptolide (TP/ GLLNP) is designed to better address the dilemma. Differing from the traditional liposomes with dual-drug co-delivery NPs, GL with a cholesterol-like structure is primarily employed to construct the lipid membrane skeleton of the GL-based lipid nanoparticle (GLLNP), and then triptolide (TP) is readily loaded in the lipid bilayer of GLLNP. The fabricated GLLNP possessed similar drug loading efficacy, particle size, and storage stability; none of the hemolysis; even higher membrane fluidity; and lower absorbed opsonin proteins compared with the conventional liposomes. Compared to TP-loaded traditional liposomes (TP/Lipo), TP/GLLNP exhibits significantly enhanced cellular uptake, cytotoxicity, and apoptosis of HepG2 cells. In addition, GLLNP could ameliorate tumor immunosuppression by promoting tumor-associated macrophage polarization from M2 to M1 phenotype. Furthermore, enhanced retention and accumulation in the tumor area of GLLNP could be found. As expected, TP/GLLNP displayed synergistic anti-hepatocellular carcinoma efficacy in vivo. In conclusion, this study provides an inspirational strategy to combine the anti-HCC benefits of GL and TP using a novel dual-drug co-delivery nanosystem.

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Section: Methodsmentioning

confidence: 99%

Section: Cellmentioning

confidence: 99%

Glycyrrhizic Acid-Lipid Framework Nanovehicle Loading Triptolide for Combined Immunochemotherapy

Xu,

Huang,

et al. 2023

ACS Appl. Mater. Interfaces

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“…To date, the influences of intrinsic properties of nanoparticles for protein corona formation have been extensively studied both in vitro and in vivo . , It has been illustrated that the intrinsic properties of nanoparticles (e.g., size, shape, surface charge, surface chemistry, and mechanical properties) play an important role in protein corona formation (Figure ). ,, Poly(ethylene glycol) (PEG) is one of the ligands frequently used to reduce protein adsorption and confer a stealth property to avoid capture from the immune system. However, anti-PEG bodies were found as the product as a consequence of increased dosage of PEG-modified nanoparticles .…”

Section: Features Of the In Vivo Protein Corona Formation Of Nanopart...mentioning

confidence: 99%

Perspectives on Protein–Nanoparticle Interactions at the In Vivo Level

He,

Qu,

Shang

2024

Langmuir

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The distinct features of nanoparticles have provided a vast opportunity of developing new diagnosis and therapy strategies for miscellaneous diseases. Although a few nanomedicines are available in the market or in the translation stage, many important issues are still unsolved. When entering the body, nanomaterials will be quickly coated by proteins from their surroundings, forming a corona on their surface, the so-called protein corona. Studies have shown that the protein corona has many important biological implications, particularly at the in vivo level. For example, they can promote the immune system to rapidly clear these outer materials and prevent nanoparticles from playing their designed role in therapy. In this Perspective, the available techniques for characterizing protein−nanoparticle interactions are critically summarized. Effects of nanoparticle properties and environmental factors on protein corona formation, which can further regulate the in vivo fate of nanoparticles, are highlighted and discussed. Moreover, recent progress on the biomedical application of protein corona-engineered nanoparticles is introduced, and future directions for this important yet challenging research area are also briefly discussed.

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“…Up to now, the evolution behavior of the protein corona in the biological environment is mostly inferred from the knowledge of the hard corona. − This is mainly attributed to the fact that NPs with a hard corona are easily isolated from biological systems for further characterization with a variety of sophisticated analytical techniques, such as mass spectrometry, circular dichroism (CD), transmission electron microscopy (TEM), and so forth . , In contrast, the soft corona is often described as a highly dynamic, loose protein layer which varies rapidly in the biological environment, but its composition and biological relevance have remained largely elusive due to the lack of analytical methods.…”

Section: Introductionmentioning

confidence: 99%

Chirality-Dependent Dynamic Evolution of the Protein Corona on the Surface of Quantum Dots

Qiao

Zhong

et al. 2022

ACS Appl. Mater. Interfaces

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Elucidating the biological behavior of engineered nanoparticles, for example, the protein corona, is important for the development of safe and efficient nanomedicine, but our current understanding is still limited due to its highly dynamic nature and lack of adequate analytical tools. In the present work, we demonstrate the establishment of a fluorescence resonance energy transfer (FRET)-based platform for monitoring the dynamic evolution behavior of the protein corona in complex biological media. With human serum albumin and lysozyme as the model serum proteins, the protein exchange process of the preformed corona on the surface of chiral quantum dots (QDs) upon feeding either individual protein or human serum was monitored in situ by FRET. Important parameters characterizing the evolution process of protein corona could be obtained upon quantitative analysis of FRET data. Further combining real-time FRET monitoring with gel electrophoresis experiments revealed that the nature of the protein initially adsorbed on the surface of QDs significantly affects the subsequent dynamic exchange behavior of the protein corona. Furthermore, our results also revealed that only a limited proportion of proteins are involved in the protein exchange, and the exchange process exhibits a significant dependence on the surface chirality of QDs. This work demonstrates the feasibility of FRET as a powerful tool to exploit the dynamic evolution process of the protein corona, which can provide theoretical guidance for further design of advanced nanomaterials for biomedical applications.

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The effect of organic ligand modification on protein corona formation of nanoscale metal organic frameworks

Cited by 14 publications

References 29 publications

Glycyrrhizic Acid-Lipid Framework Nanovehicle Loading Triptolide for Combined Immunochemotherapy

Glycyrrhizic Acid-Lipid Framework Nanovehicle Loading Triptolide for Combined Immunochemotherapy

Perspectives on Protein–Nanoparticle Interactions at the In Vivo Level

Chirality-Dependent Dynamic Evolution of the Protein Corona on the Surface of Quantum Dots

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