The Effect of Oestrogen and Progesterone on the Release of Prostaglandins From the Uterus of the Ovariectomized Guinea-Pig

Blatchley, F. R.; Poyser, N.L.

doi:10.1530/jrf.0.0400205

Cited by 65 publications

(17 citation statements)

References 6 publications

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“…The experiment with progesterone followed by oestrogen treatment was carried out because this schedule can enhance the elevation of PGs in uterine tissue and venous blood usually observed after oestrogen treatment (Blatchley et al, 1971 ;Blatchley, Donovan, Horton & Poyser, 1972 ;Saksena & Harper, 1972;Saksena & Lau, 1973;Blatchley & Poyser, 1974;Castracane & Jordan, 1975;Ham, Cirillo, Zanetti & Kuehl, 1975). This treatment also failed to influence the response of blood flow to oestrogen.…”

Section: Discussionmentioning

confidence: 99%

Uterine blood flow and prostaglandin levels in ovariectomized rabbits

Antonini¹,

Valenzuela²,

Harper³

1978

Reproduction

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Summary. Blood flow (measured by radioactive microspheres) through the uteri of ovariectomized rabbits increased significantly by 2 h, was lower at 4 and 8 h, and returned to control values by 24 h after an injection of 25 \g=m\goestradiol-17\g=b\. Blood flow through the kidneys was not affected. Concentrations of PGF and PGE in uterine vein blood were elevated after oestrogen treatment. PGF values were significantly greater than control. PGE concentrations, while elevated, were not significantly different from control, although the pattern of release was similar to that of PGF. Pretreatment of animals with progesterone or L11204, or concurrent treatment with indomethacin did not modify the blood flow through the uterus. It is concluded that the increased uterine blood flow seen after oestrogen treatment is not related to prostaglandin production.

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Section: Discussionmentioning

confidence: 99%

Uterine blood flow and prostaglandin levels in ovariectomized rabbits

Antonini¹,

Valenzuela²,

Harper³

1978

Reproduction

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“…No prostaglandin Fla was detected. (2) The later samples were assayed by radioimmunoassay using an antibody raised and tested in our laboratory (Blatchly & Poyser, 1974;Naylor & Poyser, 1975;Dighe, Emslie, Henderson, Rutherford & Simon, 1975 The uterus of a guinea-pig in dioestrus was removed and each uterine horn divided in half. The top half of one horn was combined with the bottom half of the other horn, and each uterine sample was weighed.…”

Section: Methodsmentioning

confidence: 99%

EFFECT OF p‐BROMOPHENACYL BROMIDE, AN INHIBITOR OF PHOSPHOLIPASE A₂, ON ARACHIDONIC ACID RELEASE AND PROSTAGLANDIN SYNTHESIS BY THE GUINEA‐PIG UTERUS in vitro

Mitchell

Poyser

Wilson

1977

British J Pharmacology

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The synthesis of prostaglandins F2α and E2 by guinea‐pig uterine homogenates was inhibited by p‐bromophenacyl bromide (PBPAB), an inhibitor of phospholipase A2. Metabolism of prostaglandin F2α by uterine homogenates was undetectable; this was not affected by PBPAB. There was no significant difference between the amounts of arachidonic acid released from uterine homogenates on days 7 and 15 of the oestrous cycle. Small amounts of dihomo‐γ‐linolenic acid were detected in the homogenates. The release of arachidonic acid from uterine homogenates was greatly inhibited by PBPAB. Addition of exogenous arachidonic acid to uterine homogenates did not overcome the inhibition of uterine prostaglandin F2α synthesis produced by PBPAB. It is concluded that PBPAB inhibits both the release of arachidonic acid from the guinea‐pig uterus and its subsequent conversion into prostaglandins.

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“…(55). Vertical bars join identical amino acids, two dots or one dot join highly or less highly related residues, and unjoined residues have no similarity.…”

Section: Steroidal Regulation Of Pgf 2␣ and Pgfm Secretionmentioning

confidence: 99%

Cloning of Guinea Pig Cyclooxygenase-2 and 15-Hydroxyprostaglandin Dehydrogenase Complementary Deoxyribonucleic Acids: Steroid-Modulated Gene Expression Correlates to Prostaglandin F2 Secretion in Cultured Endometrial Cells

Bracken¹

1997

Endocrinology

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Prostaglandin F 2␣ (PGF 2␣ ) secretion is lowest at midcycle and highest on day 15 at luteolysis in the cycling guinea pig uterus and is inversely related to serum progesterone levels. An increase in 17-␤ estradiol (E 2 ) occurs only towards the end of the cycle. To investigate the effect of steroids on the control of uterine PGF 2␣ metabolism at the level of gene expression we established a primary cell culture model of day 15 cycling guinea pig endometrial cells. We cloned guinea pig cDNAs for cyclooxygenase 2 (COX-2), 15-hydroxyprostaglandin dehydrogenase (PGDH) that converts PGF 2␣ to biologically inactive 13,14-dihydro-15-keto PGF 2␣ (PGFM) and a fragment of cyclooxygenase-1 (COX-1). They were found to bear 87% and 90% homology at the amino acid level to their human counterparts for COX-2 and PGDH, respectively, retaining all functional sites. Purified epithelial and stromal cell subcultures were primed with medium containing either E 2 or medroxyprogesterone acetate (MPA) for 24 h. They were then treated for a further 4 or 24 h either withdrawing the steroid, maintaining the priming steroid, or supplementing with both steroids, before harvesting conditioned media and RNA. Epithelial cells secreted 30-fold more PGF 2␣ compared with stromal cells (e.g. 7.8 Ϯ 0.7 vs. 0.26 Ϯ 0.09 pg/ng DNA•24 h), and PGF 2␣ secretion levels were approximately 15-fold higher than those of PGFM (e.g. 7.8 Ϯ 0.7 vs. 0.45 Ϯ 0.16 pg/ng DNA⅐24 h, for epithelial cells). COX-1 transcripts were low and unaffected by treatment in both cell types. COX-2 transcripts were more abundant in epithelial than stromal cells. Steroid-modulated, COX-2 dependent changes in PGF 2␣ secretion were observed. The addition of MPA to E 2 primed cells caused a decrease in PGF 2␣ secretion and COX-2 messenger RNA levels after 4 h. Conversely, the addition of E 2 to MPA primed epithelial cells led to an increase in PGF 2␣ secretion and COX-2 messenger RNA levels after 4 and 24 h. The withdrawal of E 2 caused a fall in PGF 2␣ secretion and COX-2 transcripts after 24 h. In contrast, PGDH transcripts were more abundant in stromal than epithelial cells and were up-regulated by the addition of MPA to E 2 primed cells. These in vitro observations are in keeping with the secretory profile seen in vivo in the cycling guinea pig uterus suggesting that 1) the fall of E 2 and the coinciding rise in progesterone seen in the early cycle lead to a reduction in PGF 2␣ levels; and 2) the rise of E 2 in the late cycle on a progesterone primed uterus is the stimulus for an increase in uterine PGF 2␣ production. Our findings suggest a differential role for uterine stroma and epithelium in vivo whereby the former acts to remove (via PGDH), and the latter to produce (via COX-2) biologically active prostaglandin. (Endocrinology 138: [237][238][239][240][241][242][243][244][245][246][247] 1997)

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The Effect of Oestrogen and Progesterone on the Release of Prostaglandins From the Uterus of the Ovariectomized Guinea-Pig

Cited by 65 publications

References 6 publications

Uterine blood flow and prostaglandin levels in ovariectomized rabbits

Uterine blood flow and prostaglandin levels in ovariectomized rabbits

EFFECT OF p‐BROMOPHENACYL BROMIDE, AN INHIBITOR OF PHOSPHOLIPASE A₂, ON ARACHIDONIC ACID RELEASE AND PROSTAGLANDIN SYNTHESIS BY THE GUINEA‐PIG UTERUS in vitro

Cloning of Guinea Pig Cyclooxygenase-2 and 15-Hydroxyprostaglandin Dehydrogenase Complementary Deoxyribonucleic Acids: Steroid-Modulated Gene Expression Correlates to Prostaglandin F2 Secretion in Cultured Endometrial Cells

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The Effect of Oestrogen and Progesterone on the Release of Prostaglandins From the Uterus of the Ovariectomized Guinea-Pig

Cited by 65 publications

References 6 publications

Uterine blood flow and prostaglandin levels in ovariectomized rabbits

Uterine blood flow and prostaglandin levels in ovariectomized rabbits

EFFECT OF p‐BROMOPHENACYL BROMIDE, AN INHIBITOR OF PHOSPHOLIPASE A2, ON ARACHIDONIC ACID RELEASE AND PROSTAGLANDIN SYNTHESIS BY THE GUINEA‐PIG UTERUS in vitro

Cloning of Guinea Pig Cyclooxygenase-2 and 15-Hydroxyprostaglandin Dehydrogenase Complementary Deoxyribonucleic Acids: Steroid-Modulated Gene Expression Correlates to Prostaglandin F2 Secretion in Cultured Endometrial Cells

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EFFECT OF p‐BROMOPHENACYL BROMIDE, AN INHIBITOR OF PHOSPHOLIPASE A₂, ON ARACHIDONIC ACID RELEASE AND PROSTAGLANDIN SYNTHESIS BY THE GUINEA‐PIG UTERUS in vitro