Prostaglandin F 2␣ (PGF 2␣ ) secretion is lowest at midcycle and highest on day 15 at luteolysis in the cycling guinea pig uterus and is inversely related to serum progesterone levels. An increase in 17- estradiol (E 2 ) occurs only towards the end of the cycle. To investigate the effect of steroids on the control of uterine PGF 2␣ metabolism at the level of gene expression we established a primary cell culture model of day 15 cycling guinea pig endometrial cells. We cloned guinea pig cDNAs for cyclooxygenase 2 (COX-2), 15-hydroxyprostaglandin dehydrogenase (PGDH) that converts PGF 2␣ to biologically inactive 13,14-dihydro-15-keto PGF 2␣ (PGFM) and a fragment of cyclooxygenase-1 (COX-1). They were found to bear 87% and 90% homology at the amino acid level to their human counterparts for COX-2 and PGDH, respectively, retaining all functional sites. Purified epithelial and stromal cell subcultures were primed with medium containing either E 2 or medroxyprogesterone acetate (MPA) for 24 h. They were then treated for a further 4 or 24 h either withdrawing the steroid, maintaining the priming steroid, or supplementing with both steroids, before harvesting conditioned media and RNA. Epithelial cells secreted 30-fold more PGF 2␣ compared with stromal cells (e.g. 7.8 Ϯ 0.7 vs. 0.26 Ϯ 0.09 pg/ng DNA•24 h), and PGF 2␣ secretion levels were approximately 15-fold higher than those of PGFM (e.g. 7.8 Ϯ 0.7 vs. 0.45 Ϯ 0.16 pg/ng DNA⅐24 h, for epithelial cells). COX-1 transcripts were low and unaffected by treatment in both cell types. COX-2 transcripts were more abundant in epithelial than stromal cells. Steroid-modulated, COX-2 dependent changes in PGF 2␣ secretion were observed. The addition of MPA to E 2 primed cells caused a decrease in PGF 2␣ secretion and COX-2 messenger RNA levels after 4 h. Conversely, the addition of E 2 to MPA primed epithelial cells led to an increase in PGF 2␣ secretion and COX-2 messenger RNA levels after 4 and 24 h. The withdrawal of E 2 caused a fall in PGF 2␣ secretion and COX-2 transcripts after 24 h. In contrast, PGDH transcripts were more abundant in stromal than epithelial cells and were up-regulated by the addition of MPA to E 2 primed cells. These in vitro observations are in keeping with the secretory profile seen in vivo in the cycling guinea pig uterus suggesting that 1) the fall of E 2 and the coinciding rise in progesterone seen in the early cycle lead to a reduction in PGF 2␣ levels; and 2) the rise of E 2 in the late cycle on a progesterone primed uterus is the stimulus for an increase in uterine PGF 2␣ production. Our findings suggest a differential role for uterine stroma and epithelium in vivo whereby the former acts to remove (via PGDH), and the latter to produce (via COX-2) biologically active prostaglandin. (Endocrinology 138: [237][238][239][240][241][242][243][244][245][246][247] 1997)
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