The Effect of Norepinephrine and Dibutyryl Cyclic Adenosine Monophosphate on Cation Transport in Duck Erythrocytes

Abstract

Freshly prepared duck erythrocytes, incubated either in plasma or an isotonic synthetic medium containing norepinephrine ([K] of both media 2.5 m), maintain water and electrolyte composition in the steady state (upper steady state) for at least 90 min. If incubated in the synthetic medium without norepinephrine or in plasma to which a -adrenergic blocking agent (propranolol) is added, the cells lose both water and electrolyte (predominantly KCI) until a new steady state is reached (lower steady state). Reaccumulation of water and electrolyte from isotonic solutions toward the upper steady-state levels requires the addition of norepinephrine and KCI. Reaccumulation is maximal when the concentration of K and norepinephrine in the medium is 15 mM and 10-7 M, respectively. Dibutyryl cyclic-AMP (10-2 M) mimics norepinephrine in lower steady-state cells. Although an analogous effect in upper steady-state cells was not established with certainty, it is proposed that the catecholamine-induced net changes in water and electrolyte movement in duck erythrocytes are a consequence of stimulation of the activity of a membrane-bound adenyl cyclase system.

Ouabain-insensitive salt and water movements in duck red cells. I. Kinetics of cation transport under hypertonic conditions.

“…Table III illustrates the effect of ouabain on cell water and ion contents during a 3-h incubation in isotonic solutions. In the absence of the drug, these preincu-bated cells usually continue to lose 2% of their water over the next 3 h. This is in contrast to the apparent steady state achieved by Muscovy duck red cells as reported by Riddick et al (1971). Kregenow (1973), however, has observed a similar 2% shrinkage of Muscovy duck cells under these circumstances.…”

“…These effects have also been studied in red cells from the Muscovy duck (Cairina raoschata) by Riddick and by Kregenow. Although propranolol effectively prevents the swelling response to catecholamine (Riddick et al, 1971), this /3-adrenergic blocker has no effect on reswelling in hypertonic solutions (Kregenow, 1971). The two systems demonstrate important similarities.…”

Volume-regulatory responses of Amphiuma red cells in anisotonic media. The effect of amiloride.

“…Previous reports in avian red cells indicated that catecholamines stimulate a cAMPdependent transport mechanism similar to that of the avian VRI response (Riddick et al, 1971 ;Kregenow, 1973;Kregenow et al, 1976). The possibility that hypertonicity and catecholamine-stimulated salt transport in avian and amphibian red cells may have common characteristics was anticipated by the work of Orskov (1954Orskov ( , 1956a.…”

“…Cells for gravimetric cell volume determination were removed from the top of the special tubes with a Pasteur pipette . The cell pellet was then weighed, dried at 60°C to constant weight (at least 12 h), cooled in a dessicator, and reweighed for the dry weight determination (Riddick et al ., 1971) . To obtain cells for chemical or tracer ion measurements, the plug was removed and the cells were washed out with deionized water through the opening at the base into a centrifuge tube .…”

Ouabain-insensitive salt and water movements in duck red cells. II. Norepinephrine stimulation of sodium plus potassium cotransport.

“…He found that norepinephrine and, to a lesser extent, epinephrine stimulated net potassium uptake in pigeon red cells in vitro. Riddick et al (1971) suggested that norepinephrine -is actually required for Muscovy duck red cells to maintain steady states of ions and water at normal levels in artificial, buffered salt solutions. Omission of the hormone from their incubations resulted in a spontaneous decline in cell potassium and water until a THE JOURNAL OF GENERAL PHYSIOLOGY • VOLUME 70 -1977 new "lower steady state" was reached after 90 min.…”