Volume-regulatory responses of Amphiuma red cells in anisotonic media. The effect of amiloride.

Siebens, A W; Kregenow, Floyd M.

doi:10.1085/jgp.86.4.527

Cited by 59 publications

(49 citation statements)

References 72 publications

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“…This contrasts to other cell types that progressively restore cell volume toward normal in hypertonic medium (12)(13)(14)(15)(16)(17)(18)(19)(20)(21).…”

Section: Introductionmentioning

confidence: 66%

Isovolumetric regulation of isolated S2 proximal tubules in anisotonic media.

Lohr¹,

Grantham²

1986

J. Clin. Invest.

123

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Sudden alteration in medium osmolality causes an osmometric change in proximal tubule cell size followed by restoration of cell volume toward normal in hypotonic but not in hypertonic medium. We determined the capability of isolated nonperfused proximal tubules to prevent a change in cell volume in anisotonic media. The external osmolality was gradually changed over a range from 110 to 480 mosM. At 1.5 mosM/min, cell volume remained constant between 167±9 and 361±7 mosM, a phenomenon termed isovolumetric regulation (IVR). Cells lost intracellular solutes in hypotonic and gained intracellular solutes in hypertonic media. Raffinose or choline chloride substitution showed that osmolality, rather than NaCG, signalled cell volume maintenance in hyperosmotic media. Cooling (7-100C) blocked IVR. IVR was maintained when osmolality was lowered at a rate of 27, but not at 42 mosM/min. IVR was not observed when the rate of osmolality increase exceeded 3 mosM/min. We conclude that proximal tubule cells sensitively regulate intracellular volume in an osmolality range of pathophysiologic interest by mechanisms dependent on (a) the rate of net water movement across basolateral membranes and (b) the absolute intracellular content of critical solutes.

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“…This contrasts to other cell types that progressively restore cell volume toward normal in hypertonic medium (12)(13)(14)(15)(16)(17)(18)(19)(20)(21).…”

Section: Introductionmentioning

confidence: 66%

Isovolumetric regulation of isolated S2 proximal tubules in anisotonic media.

Lohr¹,

Grantham²

1986

J. Clin. Invest.

123

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“…Duck RBC V was calculated to be ~175 fl, as 1 kg of cell dried solids equals 14.7×10 12 cells (Lytle et al, 1998), and normal cells contain 1.55-1.57 l cell water per 1 kg cell dried solids (Lytle and McManus, 2002;present study). Amphiuma RBC V and V w /A were calculated from V w =0.682 (w/w) (Siebens and Kregenow, 1985; present study), A=5000 μm…”

Section: Inhibitorsmentioning

confidence: 94%

The permeability of red blood cells to chloride, urea, and water

Brahm

2013

Journal of Experimental Biology

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SUMMARYThis study extends permeability (P) data on chloride, urea and water in red blood cells (RBC), and concludes that the urea transporter (UT-B) does not transport water. cms -1 (human). DIDS, DNDS and phloretin inhibit P Cl by >99% in all RBC species. PCMBS, PCMB and phloretin inhibit P urea by >99% in Amphiuma, dog and human RBC, but not in chick and duck RBC. PCMBS and PCMB inhibit P d in duck, dog and human RBC, but not in chick and Amphiuma RBC. Temperature dependence, as measured by apparent activation energy, E A , of P Cl is 117.8 (duck), 74.9 (Amphiuma) and 89.6kJmol −1 (dog). The E A of P urea is 69.6 (duck) and 53.3kJmol −1 (Amphiuma), and that of P d is 34.9 (duck) and 32.1kJmol −1 (Amphiuma). The present and previous RBC studies indicate that anion (AE1), urea (UT-B) and water (AQP1) transporters only transport chloride (all species), water (duck, dog, human) and urea (Amphiuma, dog, human), respectively. Water does not share UT-B with urea, and the solute transport is not coupled under physiological conditions.

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“…In salamander (Amphiuma) erythrocytes, the presence of an Na+-H+ exchanger has already been described and the physiological role has been considered (Cala, 1980;Siebens & Kregenow, 1978, 1980. In Amphiuma this exchanger becomes apparent after osmotic perturbation: when erythrocytes are placed in a hypertonic medium, their initial shrinkage is followed by a gradual re-expansion over a period of several hours.…”

Section: Na+-h+ Exchanges Induced By Catecholaminesmentioning

confidence: 99%

A transient sodium‐hydrogen exchange system induced by catecholamines in erythrocytes of rainbow trout, Salmo gairdneri.

Baroin¹,

Garcia-Romeu²,

Lamarre³

et al. 1984

The Journal of Physiology

119

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SUMMARY1. The addition of isoprenaline to an isotonic suspension of red blood cells of rainbow trout induces an amiloride-sensitive Na+ transport which is independent of Cl-and insensitive to 4,4'-diisothiocyano-2,2'-stilbene disulphonic acid (DIDS) and furosemide.2. Na+ uptake is accompanied by amiloride-sensitive H+ release. The H+ efflux is dependent upon the external Na+ concentration, the Ko.5 value for Na+ being 16 mm.3. In the presence of DIDS, when the coupled NaCl entry (NaCl co-transport) induced by catecholamine is blocked, the results provide evidence for a linked movement of Na+ and H+, with a stoicheiometry of 1 :1.4. Exchange of H+ for Na+ induces osmotic swelling of the cells which is due to the replacement of a bound proton by an osmotically active Na+ cation.5. In the absence of DIDS when the bulk of the Na+ uptake is the result of a coupled entry of Na+ and Cl-, H+ extrusion still occurs and the magnitude of acid excretion is identical to that found in DIDS-treated cells. This suggests that Na+-H+ exchange remains active.6. Addition of isoprenaline first stimulates the Na+-H+ exchange but only transiently. This is followed by a more permanent stimulation ofthe NaCl co-transport.

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Volume-regulatory responses of Amphiuma red cells in anisotonic media. The effect of amiloride.

Cited by 59 publications

References 72 publications

Isovolumetric regulation of isolated S2 proximal tubules in anisotonic media.

Isovolumetric regulation of isolated S2 proximal tubules in anisotonic media.

The permeability of red blood cells to chloride, urea, and water

A transient sodium‐hydrogen exchange system induced by catecholamines in erythrocytes of rainbow trout, Salmo gairdneri.

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