The Effect of Nondialyzable Material (NDM) Cranberry Extract on Formation of Contact Lens Biofilm by<i>Staphylococcus epidermidis</i>

Leshem, Rotem; Maharshak, Idit; Jacob, Eshel Ben; Ofek, Itzhak; Kremer, I

doi:10.1167/iovs.10-5335

Cited by 18 publications

(11 citation statements)

References 39 publications

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“…Cranberry juice and extracts have historically been used to prevent urinary tract infections in women [ 3 – 5 ]. The extracted NDM of cranberry has been shown to prevent the formation of biofilm by S. epidermis on soft contact lenses, which could reduce ocular infections [ 43 ]. It has been shown previously that cranberry NDM contains primarily A-type PACs [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…The extracted NDM of cranberry has been shown to prevent the formation of biofilm by S. epidermis on soft contact lenses, which could reduce ocular infections [ 43 ]. It has been shown previously that cranberry NDM contains primarily A-type PACs [ 43 ]. Cranberry NDM has been shown to interfere with quorum sensing in Vibrio harveyi [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

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Cranberry proanthocyanidins have anti-biofilm properties against Pseudomonas aeruginosa

Ulrey

Barksdale

Zhou

et al. 2014

BMC Complement Altern Med

119

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BackgroundBacteria within a biofilm are phenotypically more resistant to antibiotics, desiccation, and the host immune system, making it an important virulence factor for many microbes. Cranberry juice has long been used to prevent infections of the urinary tract, which are often related to biofilm formation. Recent studies have found that the A-type proanthocyanidins from cranberries have anti-biofilm properties against Escherichia coli.MethodsUsing crystal violet biofilm staining, resazurin metabolism assays, and confocal imaging, we examined the ability of A-type proanthocyanidins (PACs) to disrupt the biofilm formation of Pseudomonas aeruginosa. We used mass spectrometry to analyze the proteomic effects of PAC treatment. We also performed synergy assays and in vitro and in vivo infections to determine whether PACs, alone and in combination with gentamicin, could contribute to the killing of P. aeruginosa and the survival of cell lines and G. mellonella.ResultsCranberry PACs reduced P. aeruginosa swarming motility. Cranberry PACs significantly disrupted the biofilm formation of P. aeruginosa. Proteomics analysis revealed significantly different proteins expressed following PAC treatment. In addition, we found that PACs potentiated the antibiotic activity of gentamicin in an in vivo model of infection using G. mellonella.ConclusionsResults suggest that A-type proanthocyanidins may be a useful therapeutic against the biofilm-mediated infections caused by P. aeruginosa and should be further tested.Electronic supplementary materialThe online version of this article (doi:10.1186/1472-6882-14-499) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cranberry proanthocyanidins have anti-biofilm properties against Pseudomonas aeruginosa

Ulrey

Barksdale

Zhou

et al. 2014

BMC Complement Altern Med

119

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“…The main vehicle to cause infection is by medical device, such as catheters, heart valves, contact lenses, etc. [ 2 – 3 ]. Different strategies have been applied in order to find determinant biomarkers for S .…”

Section: Introductionmentioning

confidence: 99%

Molecular and Phenotypic Characterization of Staphylococcus epidermidis Isolates from Healthy Conjunctiva and a Comparative Analysis with Isolates from Ocular Infection

Flores-Páez

Zenteno²,

Alcántar‐Curiel

et al. 2015

PLoS ONE

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Staphylococcus epidermidis is a common commensal of healthy conjunctiva and it can cause endophthalmitis, however its presence in conjunctivitis, keratitis and blepharitis is unknown. Molecular genotyping of S. epidermidis from healthy conjunctiva could provide information about the origin of the strains that infect the eye. In this paper two collections of S. epidermidis were used: one from ocular infection (n = 62), and another from healthy conjunctiva (n = 45). All isolates were genotyped by pulsed field gel electrophoresis (PFGE), multilocus sequence typing (MLST), staphylococcal cassette chromosome mec (SCCmec), detection of the genes icaA, icaD, IS256 and polymorphism type of agr locus. The phenotypic data included biofilm production and antibiotic resistance. The results displayed 61 PFGE types from 107 isolates and they were highly discriminatory. MLST analysis generated a total of 25 STs, of which 11 STs were distributed among the ocular infection isolates and lineage ST2 was the most frequent (48.4%), while 14 STs were present in the healthy conjunctiva isolates and lineage ST5 was the most abundant (24.4%). By means of a principal coordinates analysis (PCoA) and a discriminant analysis (DA) it was found that ocular infection isolates had as discriminant markers agr III or agr II, SCCmec V or SCCmec I, mecA gene, resistance to tobramycin, positive biofilm, and IS256+. In contrast to the healthy conjunctiva isolates, the discriminating markers were agr I, and resistance to chloramphenicol, ciprofloxacin, gatifloxacin and oxacillin. The discriminant biomarkers of ocular infection were examined in healthy conjunctiva isolates, and it was found that 3 healthy conjunctiva isolates [two with ST2 and another with ST9] (3/45, 6.66%) had similar genotypic and phenotypic characteristics to ocular infection isolates, therefore a small population from healthy conjunctiva could cause an ocular infection. These data suggest that the healthy conjunctiva isolates do not, in almost all cases, infect the eye due to their large genotypic and phenotypic difference with the ocular infection isolates.

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“…To date, clinical trials have documented the efficacy of cranberry in women with recurrent UTIs (Jepson and Craig, ; Stothers, ), but there is limited research regarding the activity of cranberry on biofilm formation. Previous studies have included the evaluation of cranberry extracts on biofilm produced by Staphylococcus epidermidis on contact lens (Leshem et al , ), and cranberry juice on biofilm produced by uropathogenic E. coli (Di Martino et al , ; Tao et al , ). These studies confirm that cranberry juice can lead to a decrease in the ability of pathogenic bacteria to develop biofilm on inert surfaces such as urinary catheters and contact lenses.…”

Section: Introductionmentioning

confidence: 99%

Effects of Cranberry Extracts on Growth and Biofilm Production of Escherichia coli and Staphylococcus species

LaPlante

Sarkisian

Woodmansee

et al. 2012

Phytotherapy Research

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Biofilm producing bacteria such as Staphylococcus species and Escherichia coli are the most common cause of catheter related urinary tract infections (UTIs). The American cranberry (Vaccinium macrocarpon) is utilized widely as a prophylaxis for UTIs due to its prevention of microbial adhesion. Cranberry contains proanthocyanidins (PACs), which have been implicated as active constituents responsible for its bacterial antiadhesive properties. Despite overwhelming data supporting cranberry's beneficial effects against human pathogenic bacteria, there is limited information regarding its effects on biofilm formation. This study evaluated the effects of three proprietary PAC-standardized cranberry extracts on the inhibition of bacterial growth and biofilm production against a panel of clinically relevant pathogens: Staphylococcus epidermidis, Staphylococcus aureus, clinical methicillin-resistant S. aureus (MRSA), Staphylococcus saprophyticus and Escherichia coli. The extracts inhibited the growth of the Gram-positive bacteria (Staphylococcus spp.) but not the Gram-negative species (E. coli) with minimum inhibitory concentrations in the range 0.02-5 mg/mL. The extracts also inhibited biofilm production by the Gram-positive bacteria but did not eradicate their established biofilm. These results suggest that cranberry may have beneficial effects against the growth and biofilm producing capability of Gram-positive bacteria pathogens.

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The Effect of Nondialyzable Material (NDM) Cranberry Extract on Formation of Contact Lens Biofilm byStaphylococcus epidermidis

Abstract: NDM reduces formation of biofilm on soft contact lenses. This has important implications for the prevention of contact lens-related corneal infections caused by S. epidermidis.

Cited by 18 publications

References 39 publications

Cranberry proanthocyanidins have anti-biofilm properties against Pseudomonas aeruginosa

Cranberry proanthocyanidins have anti-biofilm properties against Pseudomonas aeruginosa

Molecular and Phenotypic Characterization of Staphylococcus epidermidis Isolates from Healthy Conjunctiva and a Comparative Analysis with Isolates from Ocular Infection

Effects of Cranberry Extracts on Growth and Biofilm Production of Escherichia coli and Staphylococcus species

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