The Effect of Nitrate and Ammonium Feeding on Carbon Dioxide Assimilation in Maize

Blackwood, G. C.; Miflin, B. J.

doi:10.1093/jxb/27.4.735

Cited by 30 publications

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“…The levels of 14C remaining in glucose-P (Fig. 4) during the chase period of NH4+-fed plants suggest that the synthesis of carbohydrates through glucose-P is reduced, in agreement with previously published results (3,13).…”

Section: Discussionsupporting

confidence: 92%

“…5). Because malate is an important metabolite in N03-transport and assimilation (1,30), it may be that in N03 -fed beans there is an increase in malate synthesis as suggested by the results and as has been demonstrated in maize (3).…”

Section: Discussionsupporting

confidence: 57%

“…Supplying C4 and C3 plants with NH4' as compared to NO3 produces, among other effects, changes in the initial carboxylation products, the CO2 compensation point, and the net photosynthetic rate (26), while in maize, NH4' nutrition causes a lower incorporation of label from 14CO2 into malate (3). Further, the addition of NH4' to N03-grown cells of Nicotiana tabacum produced an increase in the cellular concentrations of alanine and glutamine, ' This work was supported by Swiss National Science Foundation a decrease in the concentration of malate and an inhibition of malic enzyme activity (2).…”

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confidence: 99%

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Effects of Different Inorganic Nitrogen Sources on Photosynthetic Carbon Metabolism in Primary Leaves of Non-nodulated Phaseolus vulgaris L.

Marques

Oberholzer²,

Erismann³

1983

Plant Physiol.

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Young bean plants (Phaseolus vulgaris L. var Saxa) were fed with three different types of inorganic nitrogen, after being grown on nitrogen-free nutrient solution for 8 days. The pattern of "CO2 fixation was investigated in photosynthesizing primary leaf discs of 11-day-old plants (3 days with nitrogen source) and in a pulse-chase experiment in 13-day-old plants (5 days with nitrogen source).Ammonium caused, in contrast to nitrate nutrition, a higher level of 14C incorporation into sugar phosphates but a lower incorporation of label into malate, glycolate, glycerate, aspartate, and alanine. The labeling kinetics of glycine and serine were little changed by the nitrogen source. Ammonium feeding also produced an increase in the ratio of extractable activities of ribulose-1,5-bisphosphate carboxylase to phosphoenolpyruvate carboxylase and an increase in dark respiration and the CO2 compensation concentration. Net photosynthesis was higher in plants assimilating nitrate.The results point to stimulated turnover of the photosynthetic carbon reduction cycle metabolites, reduced phosphoenolpyruvate carboxylation, and altered turnover rates within the photosynthetic carbon oxidation cycle in ammonium-fed plants. Mechanisms of the regulation of primary carbon metabolism are proposed and discussed.The effect of NH4' on photosynthetic carbon assimilation has been repeatedly reported (3, 8, 12, 13, 16, 17, 19-23, 27, 29) a decrease in the concentration of malate and an inhibition of malic enzyme activity (2). However, the supply of N03-to cells of Spinacia oleracea had no apparent effect on the distribution of photosynthetic products compared with N-free nutrition, whereas in NH4t-fed cells, fixation of CO2 into carbohydrates decreased but increased in carboxylic and amino acids (16).The effect of inorganic N sources on the composition of N compounds in the bleeding sap and leaves of the bushbean Phaseolus vulgaris has previously been reported (6, 27). Coupled with the above observations, this has led us to investigate the effect of different N sources on various carboxylating enzymes and on the pattern of "4CO2 fixation in primary leaves of nonnodulated bean plants grown in liquid culture. Our results show that different N sources, fed through the roots of Phaseolus vulgaris, cause changes in photosynthetic carbon metabolism. MATERIALS AND METHODSPlant Material. Seeds of Plaseolus vulgaris L. var Saxa (strain Vatter) were soaked overnight in tap water, rinsed, and placed on wet tissue paper in darkness for 3 d at 24 ± 2°C. Seedlings were transferred to coarse quartz sand, watered with N-free nutrient (27), and grown under an illumination of 7000 to 9000 lux. After a further 4 d (day 8), the plants were set into plastic tanks filled with 14 L nutrient solution containing a total N concentration of 3.5 mm either as NH4', or NH4NO3 (27). The pH of the nutrient solution was adjusted with either KOH or H3PO4. All subsequent investigations were carried out on primary leaves. "4C02/'2C02 Pulse-Chase ExpeAments. After...

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 57%

mentioning

confidence: 99%

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Effects of Different Inorganic Nitrogen Sources on Photosynthetic Carbon Metabolism in Primary Leaves of Non-nodulated Phaseolus vulgaris L.

Marques

Oberholzer²,

Erismann³

1983

Plant Physiol.

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“…Plants grown on NH4' nutrient solutions often showed restricted growth and early senescence of their leaves, especially when the pH of the solutions was low (1,13,18). In long-term experiments, NH4' nutrition furthermore caused a lower cell sap pH and a much smaller incorporation of 14C into malate in leaves (3,16) and smaller pools of malate in leaves and roots, compared to NO3-nutrition (13,23). The extractable PEPC activity from leaves of NH4+-grown plants was found to be lower than with NO3-nutrition (16).…”

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confidence: 89%

Effect of Nitrate and Ammonium Nutrition of Nonnodulated Phaseolus vulgaris L. on Phosphoenolpyruvate Carboxylase and Pyruvate Kinase Activity

Schweizer¹,

Erismann²

1985

Plant Physiol.

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Young bean plants (Phaseolus vulgaris L. var Saxa) were fed with 3.5 or 10 millimolar N in either the form of NO3-or NH4', after being grown on N-free nutrient solution for 8 days. The pH of the nutrient solutions was either 6 or 4. The cell sap pH and the extractable activities of phosphoenolpyruvate carboxylase and of pyruvate kinase from roots and primary leaves were measured over several days.The extractable activity of phosphoenolpyruvate carboxylase (based on soluble protein) from primary leaves increased with NO3-nutrition, whereas with NHW4 nutrition and on N-free nutrient solution the activity remained at a low level. Phosphoenopyruvate carboxylase activity from the roots of NH4V-fed plants at pH 4 was finally somewhat higher than from the roots of plants grown on N03-at the same pH. There was no difference in activity from the root between the N treatments when pH in the nutrient solutions was 6. The extractable activity of pyruvate kinase from roots and primary leaves seemed not to be influenced by the N nutrition of the plants.The results are discussed in relation to the physiological function of both enzymes with special regard to the postulated functions of phosphoenolpyruvate carboxylase in C3 plants as an anaplerotic enzyme and as part of a cellular pH stat.

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“…A surplus of carbohydrate and amino acid metabolism (Paul et nitrogen can divert photosynthates away from the al, 1978), There is experiniental evidence that the formation of storage or transport carbohydrates such content of F26BP is increased when nitrogen supply as starch or sucrose to amino acid or protein synthesis is high (Selenastrum minutum\ Turpin et al, 1990; (Snyder & Tolbert, 1966;Blackwood & Miflin, Rhizinus communis: Geigenberger & Stitt, 1991). 1976Platt, Plaut & Bassham, 1977;Paul, Cornweil This could lead to a better availability of carbon & Bassham, 1978;de Veau et al, 1990de Veau et al, , 1992.…”

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confidence: 99%

Influence of different nutrient regimes on the regulation of carbon metabolism in Norway spruce [Picea abies (L.) Karst.] seedlings

et al. 1994

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SUMMARYPhosphoenolpyruvate carboxylase (PEPC) activity, fructose 2,6-bisphosphate (F26BP), starch and soluble sugar contents were determined in needles and roots of Norway spruce seedlings grown in a semi-hydroponic cultivation system under difFerent nutrient regimes. In needles, a surplus of nitrogen caused an increase in specific PEPC activity {up to six times control activity) and F26BP content (up to tbree times control level) while starch content was reduced. Sucrose contents were not affected. Basically, the responses in root samples were similar. Here, PEPC was highest at an imbalance in nutrition {-h-N/ -P). E26BP, with root contents being 3-to 11-times higher than tbose in needles, significantly exceeded control values at -f-N/ + P. The results show that aJteration of nitrogen supply leads to marked changes in allocation of carbon between pathways, which is also influenced by Pnutrition. Pool sizes of F26BP and activity of PEPC are indicators for these changes in leaf as well as in root tissues of Norway spruce.

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The Effect of Nitrate and Ammonium Feeding on Carbon Dioxide Assimilation in Maize

Cited by 30 publications

References 0 publications

Effects of Different Inorganic Nitrogen Sources on Photosynthetic Carbon Metabolism in Primary Leaves of Non-nodulated Phaseolus vulgaris L.

Effects of Different Inorganic Nitrogen Sources on Photosynthetic Carbon Metabolism in Primary Leaves of Non-nodulated Phaseolus vulgaris L.

Effect of Nitrate and Ammonium Nutrition of Nonnodulated Phaseolus vulgaris L. on Phosphoenolpyruvate Carboxylase and Pyruvate Kinase Activity

Influence of different nutrient regimes on the regulation of carbon metabolism in Norway spruce [Picea abies (L.) Karst.] seedlings

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