The Effect of Nano-Scale Topography on Keratinocyte Phenotype and Wound Healing Following Burn Injury

Parkinson, Leigh G.; Rea, Suzanne; Stevenson, Andrew; Wood, Fiona; Fear, Mark W.

doi:10.1089/ten.tea.2011.0307

Cited by 25 publications

(31 citation statements)

References 57 publications

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“…Parkinson et al demonstrated that the size of nano-pores on aluminum oxide membranes influenced the rate of keratocyte proliferation and migration in vitro [29]. In addition, the use of topographically patterned membranes as dressings lead to less granulation tissue and a more mature epidermal layer in skin than control standard burn dressings in vivo [30]. …”

Section: Discussionmentioning

confidence: 99%

The influence of substrate topography on the migration of corneal epithelial wound borders

et al. 2013

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Currently available artificial corneas can develop post-implant complications including epithelial downgrowth, infection, and stromal melting. The likelihood of developing these disastrous complications could be minimized through improved formation and maintenance of a healthy epithelium covering the implant. We hypothesize that this epithelial formation may be enhanced through the incorporation of native corneal basement membrane biomimetic chemical and physical cues onto the surface of the keratoprosthesis. We fabricated hydrogel substrates molded with topographic features containing specific bio-ligands and developed an in vitro wound healing assay. In our experiments, the rate of corneal epithelial wound healing was significantly increased by 50% in hydrogel surfaces containing topographic features, compared to flat surfaces with the same chemical attributes. We determined that this increased healing is not due to enhanced proliferation or increased spreading of the epithelial cells, but to an increased active migration of the epithelial cells. These results show the potential benefit of restructuring and improving the surface of artificial corneas to enhance epithelial coverage and more rapidly restore the formation of a functional epithelium.

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Section: Discussionmentioning

confidence: 99%

The influence of substrate topography on the migration of corneal epithelial wound borders

et al. 2013

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“…4 5 AAO can form different types of anodic oxides: a non-porous barrier type, when fabricated using neutral electrolytes, and a porous-type oxide, when fabricated using acidic electrolytes. Nanoporous alumina is a suitable template for the production of nanostructures 6 and has been used in medical applications, e.g., tissue engineering, in recent years based on reports that various types of cells, such as neurons, 7 hepatic cells, 8 smooth muscle cells, 9 keratinocytes, 10 fibroblasts, 11 and osteoblasts, 12 are capable of attaching to its surface. Furthermore, neurite outgrowth is known to be accelerated by pitch-dependent AAO substrates, 7 and hepatic cell growth is accelerated on self-supporting, mechanically stabilized nanoporous aluminum oxide membranes.…”

Section: Introductionmentioning

confidence: 99%

Cell Adhesion and Growth on the Anodized Aluminum Oxide Membrane

Park¹,

Moon²,

Kim³

et al. 2016

j biomed nanotechnol

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Nanotopological cues are popular tools for in vivo investigation of the extracellular matrix (ECM) and cellular microenvironments. The ECM is composed of multiple components and generates a complex microenvironment. The development of accurate in vivo methods for the investigation of ECM are important for disease diagnosis and therapy, as well as for studies on cell behavior. Here, we fabricated anodized aluminum oxide (AAO) membranes using sulfuric and oxalic acid under controlled voltage and temperature. The membranes were designed to possess three different pore and interpore sizes, AAO-1, AAO-2, and AAO-3 membranes, respectively. These membranes were used as tools to investigate nanotopology-signal induced cell behavior. Cancerous cells, specifically, the OVCAR-8 cell-line, were cultured on porous AAO membranes and the effects of these membranes on cell shape, proliferation, and viability were studied. AAO-1 membranes bearing small sized pores were found to maintain the spreading shape of the cultured cells. Cells cultured on AAO-2 and AAO-3 membranes, bearing large pore-sized AAO membranes, changed shape from spreading to rounding. Furthermore, cellular area decreased when cells were cultured on all three AAO membranes that confirmed decreased levels of focal adhesion kinase (FAK). Additionally, OVCAR-8 cells exhibited increased proliferation on AAO membranes possessing various pore sizes, indicating the importance of the nanosurface structure in regulating cell behaviors, such as cell proliferation. Our results suggest that porous-AAO membranes induced nanosurface regulated cell behavior as focal adhesion altered the intracellular organization of the cytoskeleton. Our results may find potential applications as tools in in vivo cancer research studies.

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“…A previous study using keratinocytes grown on aluminum oxide nanoporous membranes also showed that greater cell spreading correlated with higher levels of proliferation. 15 Therefore, we hypothesized that decreased cell spreading will lead to lower proliferation on the SA samples compared with the other substrates in the study.…”

Section: Resultsmentioning

confidence: 99%

“…A previous study using aluminum oxide membranes showed that nanopore size influences keratinocyte proliferation and migration, both of which affect wound repair and reconstruction. 15 Another study using fibroblasts cultured on polymer microrods showed that microtopography affected cell proliferation and downregulated synthesis of matrix proteins involved in scar formation. 16 Additionally, previous work in our group using corneal fibroblasts cultured on collagen nanofibrillar substrates has shown that both fibril diameter and alignment promote the quiescent keratocyte phenotype and downregulate matrix synthesis.…”

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confidence: 99%

Effect of Collagen Nanotopography on Keloid Fibroblast Proliferation and Matrix Synthesis: Implications for Dermal Wound Healing

Muthusubramaniam

Zaitseva

Paukshto

et al. 2014

Tissue Engineering Part A

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Keloids are locally exuberant dermal scars characterized by excessive fibroblast proliferation and matrix accumulation. Although treatment strategies include surgical removal and intralesional steroid injections, an effective regimen is yet to be established due to a high rate of recurrence. The regressing center and growing margin of the keloid have different collagen architecture and also differ in the rate of proliferation. To investigate whether proliferation is responsive to collagen topography, keloid, scar, and dermal fibroblasts were cultured on nanopatterned scaffolds varying in collagen fibril diameter and alignment-small and large diameter, aligned and random fibrils, and compared to cells grown on flat collagen-coated substrates, respectively. Cell morphology, proliferation, and expression of six genes related to proliferation (cyclin D1), phenotype (asmooth muscle actin), and matrix synthesis (collagens I and III, and matrix metalloproteinase-1 and -2) were measured to evaluate cell response. Fibril alignment was shown to reduce proliferation and matrix synthesis in all three types of fibroblasts. Further, keloid cells were found to be most responsive to nanotopography.

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The Effect of Nano-Scale Topography on Keratinocyte Phenotype and Wound Healing Following Burn Injury

Cited by 25 publications

References 57 publications

The influence of substrate topography on the migration of corneal epithelial wound borders

The influence of substrate topography on the migration of corneal epithelial wound borders

Cell Adhesion and Growth on the Anodized Aluminum Oxide Membrane

Effect of Collagen Nanotopography on Keloid Fibroblast Proliferation and Matrix Synthesis: Implications for Dermal Wound Healing

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