Cell Adhesion and Growth on the Anodized Aluminum Oxide Membrane

Park, Jeong Su; Moon, Dalnim; Kim, Jin-Seok; Lee, Jin Seok

doi:10.1166/jbn.2016.2192

Cited by 13 publications

(11 citation statements)

References 23 publications

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“…When the pores are smaller than this critical size, the AAO surface is not favorable for cell adhesion but does not induce cell death. These are generally in agreement with the related literature works . For instance, Song et al .…”

Section: Resultssupporting

confidence: 93%

“…Park et al . showed that AAO does not have any cell toxicity and that cell proliferation is enhanced as the pore size increases up to 30 nm but larger pores (40 nm) decreases the enhancement effect . On the contrary, Taxis et al .…”

Section: Resultsmentioning

confidence: 96%

“…Various aspects of AAO such as pore size, pore depth, and surface wettability have been explored for their effects on biological entities, leading to a general conclusion that the AAO surfaces can affect biological systems . For instance, cell adhesion and/or cell proliferation are reported to depend strongly on the pore size or the pore depth of AAO membranes as demonstrated with human cell lines such as HMEC cells, HepG2 cells, NIH3T3 cells, hFOB 1.19 cells, and OVCAR‐8 cells . However, how the AAO surface interacts with cells is still unclear, which information can be critically important for the development of biocompatible nanoporous materials.…”

Section: Introductionmentioning

confidence: 99%

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Viability Studies of Cells on Nanostructured Surfaces With Various Feature Sizes

Kim

Lee

et al. 2017

Bulletin Korean Chem Soc

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We demonstrate that the pore size of a nanoporous anodic aluminum oxide (AAO) membrane is critical for cell viability and cell adhesion upon HeLa cell growth on it, while other properties of membranes such as surface wettability and pore fraction show no such effects. After a human cancer cell, HeLa cell line, was seeded on AAO membranes with various pore sizes ranging from 18 to 150 nm, the adhered cell population and 3‐(4,5‐dimethyltiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay were measured. The cell adhesion and viability on the membrane with small pores (18 nm) were similar to those on the nonporous reference; however, they gradually decreased as the pore size increased. The decreased viability involves cell death as well as poor cell adhesion. We found that cyclo(l‐arginylglycyl‐l‐α‐aspartyl‐d‐phenylalanyl‐l‐cysteinyl), a well‐known integrin receptor antagonist, drastically decreased the cell adhesion to the membranes. The data indicate that the cell adhesion to the nanoporous membranes may be mediated by integrin on the cell surface, plausibly explaining the pore size dependence of the cell adhesion and viability. Implications of the present results to related fields such as implants are discussed.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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Viability Studies of Cells on Nanostructured Surfaces With Various Feature Sizes

Kim

Lee

et al. 2017

Bulletin Korean Chem Soc

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“…In their case, cells cultured on 30 nm sized nanoporous surfaces proliferated much better than those on flat surfaces and other nanoporous surfaces (40, 45, and 50 nm). Park et al (2016) fabricated anodic aluminum oxide (AAO) membranes, which were designed to possess three different pore sizes, AAO-1 ($25 nm), AAO-2 ($33 nm), and AAO-3 ($46 nm). AAO-1 membranes bearing small sized pores were found to maintain the spreading shape of the cultured cells, while cells cultured on AAO-2 and AAO-3 membranes, bearing large pore-sized AAO membranes, changed shape from spreading to rounding.…”

Section: Nanoporesmentioning

confidence: 99%

Biological responses to nanomaterials: understanding nano-bio effects on cell behaviors

Liu

Tang

2017

Drug Delivery

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The unique properties of nanomaterials in drug delivery and tissue engineering have captured a great deal of attention as experimental tools in bioimaging, diagnostic, and therapeutic processes. A plenty of research have provided a strong evidence that nanostructures not only passively interact with cells but also actively engage and mediate cell functions and molecular processes. Undoubtedly, it is crucially important to better understand biological responses to engineered nanomaterials, especially in view of their potential for biomedical applications. In this review, we shall highlight recent advances in exploring nano-bio effects in diverse systems of nanoparticles, nanotopographies, and mixed composite scaffolds. We will also discuss their manipulating functions on cellular behaviors and important biological processes of adhesion, morphology, proliferation, migration, differentiation, and even hidden mechanisms including molecular signaling pathways. At last, the perspectives will be addressed for further directions of nanomaterial designs with the purpose of better drug delivery and cell therapies. ARTICLE HISTORY

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“…However, the duration of release is dependent on the amount of protein loaded and the bioactive molecules in the coating; that is, it is limited. Therefore, Rough surfaces, especially nanostructured surfaces, maintain neuron viability more effectively than smooth surfaces, presumably because the former mimic the extracellular matrix [8,9]. Various nanostructured surfaces-such as nanowire arrays [10,11], nanopatterned poly-ε-caprolactone (PCL) [12], and a nanoporous gold (np-Au) substrate [13]-increased neuronal growth compared with that on a flat surface.…”

Section: Introductionmentioning

confidence: 99%

An SU-8-based microprobe with a nanostructured surface enhances neuronal cell attachment and growth

Kim

Choi

2017

Micro and Nano Syst Lett

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Microprobes are used to repair neuronal injury by recording electrical signals from neuronal cells around the surface of the device. Following implantation into the brain, the immune response results in formation of scar tissue around the microprobe. However, neurons must be in close proximity to the microprobe to enable signal recording. A common reason for failure of microprobes is impaired signal recording due to scar tissue, which is not related to the microprobe itself. Therefore, the device-cell interface must be improved to increase the number of neurons in contact with the surface. In this study, we developed nanostructured SU-8 microprobes to support neuronal growth. Nanostructures of 200 nm diameter and depth were applied to the surface of microprobes, and the attachment and neurite outgrowth of PC12 cells on the microprobes were evaluated. Neuronal attachment and neurite outgrowth on the nanostructured microprobes were significantly greater than those on non-nanostructured microprobes. The enhanced neuronal attachment and neurite outgrowth on the nanostructured microprobes occurred in the absence of an adhesive coating, such as poly-l-lysine, and so may be useful for implantable devices for long-term use. Therefore, nanostructured microprobes can be implanted without adhesive coating, which can cause problems in vivo over the long term.

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Cell Adhesion and Growth on the Anodized Aluminum Oxide Membrane

Cited by 13 publications

References 23 publications

Viability Studies of Cells on Nanostructured Surfaces With Various Feature Sizes

Viability Studies of Cells on Nanostructured Surfaces With Various Feature Sizes

Biological responses to nanomaterials: understanding nano-bio effects on cell behaviors

An SU-8-based microprobe with a nanostructured surface enhances neuronal cell attachment and growth

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