The effect of multiple cycles in oocyte donors

Jain, Akas; Robins, Jared C.; Williams, Daniel B.; Thomas, Michael A.

doi:10.1016/j.ajog.2004.12.038

Cited by 7 publications

(4 citation statements)

References 6 publications

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“…Other studies also indicate that high peak E 2 levels are not detrimental to oocyte quality, fertilization, and embryo cleavage; and that to the contrary, elevated E 2 levels result in a greater number of oocytes and embryos for selection at the time of embryo transfer or cryopreservation [1,3,14,20,21]. These findings are in agreement with observations for high responder (those with peak E 2 >2000) undergoing conventional in vitro fertilization.…”

Section: Discussionsupporting

confidence: 82%

Effect of estradiol on oocyte development

Bianco

Mahutte

Arıcı

et al. 2008

Intl J Gynecology & Obste

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Objective To determine whether elevated serum estradiol (E2) concentrations in oocyte donors affect assisted reproduction outcome. Method In a retrospective cohort study of 58 consecutive oocyte donation cycles, donors were stratified into 2 groups according to E2 concentration, group 1 (n=32; E2≤2000 pg/mL [range 456–2000 pg/mL]) and group 2 (n=27; E2>2000 pg/mL [range, 2062–6957 pg/mL]). Data were analyzed using the t test and χ2 test. Results Donors in group 1 produced significantly less oocytes than donors in group 2 (19.3±1.7 vs 12.0±1.4; P<0.001), and recipients of oocytes from group 1 had significantly fewer numbers of embryos available for transfer (10.4±1.1 vs 6.4±0.8; P=0.003). However, the mean number (3.3) of embryos transferred and the pregnancy rate were the same in both groups. Conclusion Elevated estradiol concentration in oocyte donors did not affect pregnancy outcome, suggesting that estradiol levels in donors do not affect oocyte development.

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Section: Discussionsupporting

confidence: 82%

Effect of estradiol on oocyte development

Bianco

Mahutte

Arıcı

et al. 2008

Intl J Gynecology & Obste

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“…The same results were found in humans, that the oocyte quality, assessed as fertilization, implantation and pregnancy rates, was similar through three to five repeated controlled ovarian stimulation cycles (Caligara et al, 2001;Jain et al, 2005). This shows that there is no difference in the quality of the matured oocytes retrieved from monkeys in first and repeat cycles.…”

Section: Effect Of Stimulation Cycle On Scntsupporting

confidence: 79%

Somatic cell nuclear transfer using transported in vitro-matured oocytes in cynomolgus monkey

Chen

Liow

Abdullah

et al. 2007

Zygote

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Somatic cell nuclear transfer (SCNT) is not successful so far in non-human primates. The objective of this study was to investigate the effects of stimulation cycles (first and repeat) on oocyte retrieval and in vitro maturation (IVM) and to evaluate the effects of stimulation cycles and donor cell type (cumulus and fetal skin fibroblasts) on efficiency of SCNT with transported IVM oocytes. In this study, 369 immature oocytes were collected laparoscopically at 24 h following human chorionic gonadotrophin (hCG) treatment from 12 cynomolgus macaque (Macaca fascicularis) in 24 stimulation cycles, and shipped in pre-equilibrated IVM medium for a 5 h journey, placed in a dry portable incubator (37 degrees C) without CO(2) supplement. A total of 70.6% (247/350) of immature oocytes reached metaphase II (MII) stage at 36 h after hCG administration, MII spindle could be seen clearly in 80.6% (104/129) of matured IVM oocytes under polarized microscopy. A total of 50.0% (37/74) of reconstructive SCNT embryos cleaved after activation; after cleavage, 37.8% (14/37) developed to the 8-cell stage and 8.1% (3/37) developed to morula, but unfortunately none developed to the blastocyst stage. Many more oocytes could be retrieved per cycle from monkeys in the first cycle than in repeated cycles (19.1 vs. 11.7, p < 0.05). There were no significant differences in the maturation rate (70.0 vs. 71.4%, p > 0.05) and MII spindle rate under polarized microscopy (76.4 vs. 86.0%, p > 0.05) between the first and repeat cycles. There were also no significant differences in the cleavage rate, and the 4-cell, 8-cell and morula development rate of SCNT embryos between the first and repeat cycles. When fibroblast cells and cumulus cells were used as the donor cells for SCNT, first cleavage rate was not significantly different, but 4-cell (50.0 vs. 88.9%, p < 0.05) and 8-cell (0 vs. 51.9%, p < 0.01) development rate were significantly lower for the former. In conclusion, the number of stimulation cycles has a significant effect on oocyte retrieval, but has no effect on maturation and SCNT embryo development; however, different donor cell types (cumulus and fibroblast) resulted in different developmental potentials of SCNT embryos.

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“…Although several previous studies have investigated the ovarian response by comparing outcomes of subsequent cycles in a period of several years ( 12 , 13 ), firm conclusions about the effect of ovarian stimulation can only be drawn if repetitive cycles are performed ideally within a short time frame, using the same stimulation strategy (e.g., type of gonadotropin, GnRH analog protocol, and decisions on patient management). In this regard, evidence derived from oocyte donation cycles has shown that the ovarian response is not altered in case a subsequent IVF cycle is started in a short period after the first attempt ( 14 , 15 ). However, these studies evaluated the effect of stimulation on reproductive outcomes without taking into account the ovarian stimulation regimen, which is the most important parameter for decision-making.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Dose Adjustments in a Subsequent Cycle of Women With Suboptimal Response Following Conventional Ovarian Stimulation

et al. 2018

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Several infertile patients, who may even represent around 40% of the infertile cohort, may respond “suboptimally” (4–9 oocytes retrieved) following IVF, despite being predicted as normal responders. The aim of our longitudinal study was to evaluate the ovarian response of suboptimal responders in terms of the number of oocytes retrieved, following their second IVF cycle, evaluating exclusively patients who had the same stimulation protocol and used the same or higher initial dose of the same type of gonadotropin compared to their previous failed IVF attempt. Overall, our analysis included 160 patients treated with a fixed antagonist protocol in their second cycle with the same [53 (33.1%)] or higher [107 (66.9%)] starting dose of rFSH. The number of oocytes retrieved was significantly higher in the second IVF cycle [6 (5–8) vs. 9 (6–12), p < 0.001]. According to our results, a dose increment of rFSH remained the only significant predictor of the number of oocytes retrieved in the subsequent IVF cycle (coefficient 0.02, p-value = 0.007) after conducting GEE multivariate regression, while adjusting for relevant confounders. A regression coefficient of 0.02 for the starting dose implies that an increase of 50 IU of the initial rFSH dose would lead to 1 more oocyte.

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The effect of multiple cycles in oocyte donors

Cited by 7 publications

References 6 publications

Effect of estradiol on oocyte development

Effect of estradiol on oocyte development

Somatic cell nuclear transfer using transported in vitro-matured oocytes in cynomolgus monkey

The Effect of Dose Adjustments in a Subsequent Cycle of Women With Suboptimal Response Following Conventional Ovarian Stimulation

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