The effect of molybdenum on the<i>in vitro</i>development of mouse preimplantation embryos

Bi, Chen; Zhang, Yuling; Liu, Fengjun; Zhou, Tiezhong; Yang, Zhihong; Gao, Shenyang; Wang, Shu-De; Chen, Xiaoli; Zhai, Xiaowei; Ma, Xue-Gang; Jin, Lijun; Wang, Shen

doi:10.3109/19396368.2012.743198

Cited by 20 publications

(7 citation statements)

References 27 publications

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“…Molybdenum is very toxic to ruminants at levels of several parts per million being the most affected are cows (Underwood 1979;Kincaid 1980). Molybdenum toxicity in inhibiting spermatogenesis and arresting embryogenesis in organisms such as catfish and mice at levels as low as several parts per million have been reported (Meeker et al 2008;Bi et al 2013;Zhai et al 2013;Zhang et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a molybdenum-reducing and SDSdegrading Klebsiella oxytoca strain Aft-7 and its bioremediation application in the environment

Masdor¹,

Shukor²,

Khan

et al. 2015

Biodiversitas

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Pollution as a result of anthropogenic activities is a severe global issue. These activities including inappropriate disposal, industrial and prospecting activities and unnecessary use of agricultural chemicals have triggered international initiatives to eliminate these contaminants. In this work we screen the ability of a molybdenum-reducing bacterium isolated from contaminated soil to grow and reduce molybdenum on various detergents. The bacterium was able to grow on SDS as a carbon source although the compound did not support molybdenum reduction. The bacterium reduces molybdate to Mo-blue optimally between pH 5.8 and 6.3 and between 25 and 34 o C. Glucose was the best electron donor for supporting molybdate reduction followed by sucrose, D-mannitol, D-sorbitol, lactose, salicin, trehalose, maltose and myo-Inositol in descending order. Other requirements include a phosphate concentration between 5.0 and 7.5 mM and a molybdate concentration between 5 and 20 mM. The absorption spectrum of the Mo-blue produced was similar to previous Mo-reducing bacterium, and closely resembles a reduced phosphomolybdate. Molybdenum reduction was inhibited by mercury (ii), silver (i) and copper (ii) at 2 ppm by 62.1, 33.9 and 33.6%, respectively. Biochemical analysis resulted in a tentative identification of the bacterium as Klebsiella oxytoca strain Aft-7. The ability of this bacterium to detoxify molybdenum and degrade detergent makes this bacterium an important tool for bioremediation.

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Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a molybdenum-reducing and SDSdegrading Klebsiella oxytoca strain Aft-7 and its bioremediation application in the environment

Masdor¹,

Shukor²,

Khan

et al. 2015

Biodiversitas

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“…Molybdenum is commonly used in the industry and causes many instances of water poisoning worldwide such as in Malaysia [3], the Tokyo Bay, Tyrol in Austria and the Black Sea [4,5]. Toxicity to molybdenum has been recorded as low as a few parts per million resulting in the inhibition of spermatogenesis and embryonic in a number of species including catfish and mouse [6,[6][7][8][9]. In addition, molybdenum is extremely poisonous This work is licensed under the terms of the Creative Commons Attribution (CC BY) (http://creativecommons.org/licenses/by/4.0/).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of a Molybdenum-reducing and the Congo Red Dye-decolorizing Pseudomonas putida strain Neni-3 in soils from West Sumatera, Indonesia

Rusnam

Gusmanizar

2022

J Biochem Microbiol Biotechnol

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The elimination of heavy metals and organic contaminants, such as phenols, hydrocarbons, and amides, by bioremediation, is the most effective choice for the foreseeable future. This is especially true at low levels, where other methods, such as physical or chemical methods, may not be successful. Each year, a few million tons of these contaminants are emitted. In this study, we examined the ability of a molybdenum-reducing bacteria that were isolated from polluted soil to decolorize azo dyes independently of its ability to reduce molybdenum. The ideal conditions for the bacterium to convert molybdate to molybdate blue are a pH range of 6.0 to 6.5 and a temperature range of 25 to 37 degrees Celsius. After glucose, fructose and galactose were the most effective donors of electrons to enable the reduction of molybdate. Galactose was the least effective supplier of electrons. There are a few other prerequisites that need to be met as well, such as a phosphate concentration of between 2.5 and 7.5 mM and a molybdate concentration of between 10 and 15 mM. Its absorption spectra were identical to that of the phosphomolybdate reduction process and to that of the earlier Mo-reducing bacterium. At a concentration of 2 ppm, the heavy metals Ag (I), Hg (II), and Cu (II) each inhibited the reduction of molybdenum by a per centage of 62.8, 61.1, and 36.8 per cent, respectively. We put the bacterium through a test to see if it can remove the color from a variety of dyes. The Congo Red dye was able to lose its color when exposed to the bacterium. Based on the results of the biochemical study, the bacterium has been provisionally identified as Pseudomonas putida strain Neni-3. This bacteria's ability to detoxify various toxicants is a desirable quality, as it makes the bacterium an efficient bioremediation approach. As a result, this bacterium is in high demand. Purification of the molybdenum-reducing enzyme that was produced by this bacterium is presently being studied in order to characterize decolorization research in a more accurate manner.

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“…This discovery came about during that decade. It has been demonstrated that even at concentrations as low as a few parts per million, molybdenum may inhibit the development of embryos and delay the process of spermatogenesis in various species, including catfish and mice [6,[6][7][8][9]. In addition, molybdenum is extremely hazardous to ruminants, particularly cows, at levels of several parts per million.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Molybdenum-reducing and Phenol-degrading Pseudomonas sp. strain Neni-4 from soils in West Sumatera, Indonesia

Rusnam

Gusmanizar

Rahman

et al. 2022

Bull Environ Sci Sust Manage

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Millions of tonnes of these chemicals are manufactured each year and a large quantity is determined to be contaminating the environment, making them important worldwide pollutants. The fact that they pollute the environment is a major problem on a worldwide scale. There is a continuing search for bioremediation of these contaminants employing bacteria capable of numerous detoxifications. Analysis of the bacterium yielded a preliminary identification of the organism as Pseudomonas aeruginosa Neni-4. Screening for the capacity of molybdenum-reducing bacteria to decolorize different polyphenols was conducted in this study. Reduction was optimum at pH 6.3 and between 25 and 40 oC. The bacterium used glucose as the best carbon source or molybdenum reduction followed by galactose, 2-ketogluconate, and citrate in decreasing order. Phosphate between 5.0 and 7.5 mM and sodium molybdate between 15 and 20 mM maximally supported reduction. Like earlier Mo-reducing bacteria, a reduction of phosphomolybdate is seen in the absorption spectra of the Mo-blue generated. Heavy metals prevented molybdenum reduction. None of the phenolic compounds can reduce molybdenum when provided as sole carbon sources. In contrast, the bacterium was able to grow on phenol, benzoate, salicylic acid, and catechol, all of which are substances that include phenolic components. A significant bioremediation technology is this bacterium's capacity to metabolise molybdenum and thrive on poisonous phenolics.

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The effect of molybdenum on thein vitrodevelopment of mouse preimplantation embryos

Cited by 20 publications

References 27 publications

Isolation and characterization of a molybdenum-reducing and SDSdegrading Klebsiella oxytoca strain Aft-7 and its bioremediation application in the environment

Isolation and characterization of a molybdenum-reducing and SDSdegrading Klebsiella oxytoca strain Aft-7 and its bioremediation application in the environment

Isolation and Characterization of a Molybdenum-reducing and the Congo Red Dye-decolorizing Pseudomonas putida strain Neni-3 in soils from West Sumatera, Indonesia

Characterization of a Molybdenum-reducing and Phenol-degrading Pseudomonas sp. strain Neni-4 from soils in West Sumatera, Indonesia

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