The effect of mixing intensity on the performance and microbial dynamics of a single vertical reactor integrating acidogenic and methanogenic phases in lignocellulosic biomass digestion

Zhao, Ye; Wu, Jingwei; Yuan, Xufeng; Zhu, Wanbin; Wang, Xiaofen; Xu, Chuncheng; Cui, Zongjun

doi:10.1016/j.biortech.2017.04.080

Cited by 43 publications

(13 citation statements)

References 26 publications

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“…The significant rise in acetic acid and propionic acid was noted in the digester with intensive mixing, and this resulted in a fast diffusion from top to bottom. The substrate type, mixing intensity and the structure and volume of the reactor affected biogas production efficiency [39]. Supporting this fact, Stroot et al [19] determined that the level of VFAs which increased sharply under continuous mixing conditions because of an increase of acetate concentrations, which was due to an imbalance in the digestion process at higher mixing intensities.…”

Section: Effect Of Mixing On Vfa Concentration and Tvs Reductionmentioning

confidence: 96%

“…Various rheological models were developed, such as the Casson model, the power law and the Bingham plastic model. A non-Newtonian fluid character of slurry Ghanimeh et al [18] Latha et al [42] Nasir et al [44] Hoffamn et al [15] Sulaiman et al [45] Stroot et al [19] Tian et al [22] Lind et al [46] Si-Jia et al [17] Yu et al [39] VFA concentraƟon [g/l] Graph 2. Presents the effect of different mixing strategies on accumulation of TVFAs in the anaerobic digestion process.…”

Section: Effect Of Viscosity Shear Stress and Ts Contentmentioning

confidence: 99%

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Impact of mixing intensity and duration on biogas production in an anaerobic digester: a review

Singh

Szamosi

Siménfalvi

2020

Critical Reviews in Biotechnology

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This paper is a critical evaluation of the effect of mixing on biogas production rates in an anaerobic digester. Mixing plays a prominent role in determining the efficiency of the anaerobic digestion process. This review analyzes the miscellaneous effects of mixing (on the microbial community, methane content and volatile fatty acids) at various mixing intensities and during different stages of the digestion process. Intermittent mixing (mixing at intervals) seems preferable in terms of the quality and quantity of biogas produced, and results in lower power consumption and maintenance costs associated with large-scale biogas production. Preferable mixing time (the length at intervals) and the intensity depends on the geometry of the digester and impeller. The conclusion is drawn that the study of the slurry rheology is very crucial in the designing of the mixing equipment, the shape and size of the digester, and the pipe transport system which can assist in minimizing the initial investment and operational costs. Accordingly, this paper focuses on the parameters which determine the potency of mixing, such as viscosity, total solid content and digester design. Empirical data demonstrated by various researchers regarding rheological characteristics is compared and reviewed. Consequently, close attention should be paid toward the optimization of mixing in terms of its speed, mixing time and impeller geometry, especially during different stages of the digestion process (hydrolysis, acidogenesis, acetogenesis and methanogenesis). Finally, readers will be guided to the extensive publications regarding optimization, directions of future research, and troubleshooting of the mixing operation in an anaerobic digester. This investigation will help to improve mixing efficiency with biogas plants. HIGHLIGHTSEffect of shear rate on different stages of an anaerobic digestion process. Methane content varies with the variation in mixing speeds. Mixing effect is significant when the total solid content is higher. Intermittent mixing is favorable when compared to continuous mixing. The geometry of the digester and mixer is essential to evaluate digester mixing. ARTICLE HISTORY

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Section: Effect Of Mixing On Vfa Concentration and Tvs Reductionmentioning

confidence: 96%

Section: Effect Of Viscosity Shear Stress and Ts Contentmentioning

confidence: 99%

Impact of mixing intensity and duration on biogas production in an anaerobic digester: a review

Singh

Szamosi

Siménfalvi

2020

Critical Reviews in Biotechnology

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“…Frontiers in Microbiology | www.frontiersin.org cycles of 95 The PCR products were purified using a QIAquick Gel Extraction Kit (QIAGEN, Germany), quantified by a real-time PCR, and sequenced on an Illumina MiSeq PE300 platform at Beijing Allwegene Tech, Ltd (Beijing, China). After the run, image analysis, base calling, and error estimation were performed using Illumina Analysis Pipeline Version 2.6 (Zhao et al, 2017). The erroneous and chimeric sequences were filtered out, and pair-end reads were initially trimmed using Illumina Analysis Pipeline Version 2.6 to remove low-quality sequences with a score below 20.…”

Section: Dna Extraction Pcr Amplification and Illumina Sequencingmentioning

confidence: 99%

Adaptive Pathways of Microorganisms to Cope With the Shift From P- to N-Limitation in Subtropical Plantations

Wang

Jiao

2022

Front. Microbiol.

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Ecological stoichiometry is increasingly acknowledged as one of the main control factors for microbial activity and diversity. Soil carbon/nitrogen (C/N) and carbon/phosphorus (C/P) ratios are usually much higher than microbial nutrient requirements and vary with planting density and stand age in forestlands. However, how microorganisms cope with such stoichiometric imbalances and how they regulate nutrient cycling remain unclear. Here, 5- and 35-year-old experimental Cunninghamia lanceolata [Lamb.] Hook plantations with five planting densities (1,667, 3,333, 5,000, 6,667, and 10,000 stems ha−1) were used to explore the underlying mechanism of the response of microorganisms to stoichiometric imbalances. We found that (i) enzyme activity and microbial biomass and diversity increased with planting density at age 5 but decreased at age 35; (ii) soil microorganisms were P-limited at age 5, but gradually shifted from P- to N-limitation during the development of plantations from 5 to 35 years; and (iii) significantly negative relationships between microbial biomass stoichiometry and enzymatic stoichiometry were observed. The adaptive pathways of soil microorganisms to cope with stoichiometric imbalances include (i) adjusting the stoichiometry of microorganisms and enzymes; (ii) changing the relative abundance of the dominant microbial phyla; and (iii) increasing the ratio of fungal to bacterial diversity. These results highlight how to use the ecological stoichiometry method to identify soil microbial nutrient limitations with planting density during the development of plantations. By underlining the important role of stoichiometry on microbial growth and activity, these findings furthermore emphasize the dependency of organic matter transformation and nutrient cycling on the microbial community.

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“…Members of this order, such as Clostridium sp. have already been described with the ability to consume different carbon sources through hydrolysis, culminating with organic acids and hydrogen generation, among other metabolic pathways [51]. The high relative abundance of the order Clostridiales in reactor R3 (10.6%) suggests there were still compounds to be consumed or an environmental condition that could favor/select these endospore-forming cells.…”

Section: Microbial Relative Abundancementioning

confidence: 99%

Bioconversion of Crude Glycerol to Hydrogen and Methane in Three-Stage Horizontal Anaerobic Fixed-Bed Reactors (HARFB)

Adames

Jacobus

Sakamoto

et al. 2021

Preprint

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The anaerobic conversion of residual glycerol from biodiesel production is an excellent option for producing clean energy at a low cost. Crude glycerol (CG) is an excellent carbon source for microorganisms in Anaerobic Digestion (AD) process; however, it is a nutrient-limited waste. On the other hand, domestic Sewage (DS) is wastewater containing macronutrients, such as nitrogen and phosphorus. Therefore, feeding bioreactors with a blend of both wastes is an exciting approach. The objective of this study was to operate a system consisting of three horizontal anaerobic series reactors (HARFB) with fixed bed (R1, R2, and R3), fed with a mixture of crude glycerol and domestic sewage to evaluate the start-up strategy and best organic loading rate (OLR) to improve biogas production (H2 e CH4) as well as to analyze the dynamic changes in the anaerobic microbial consortium during the operation of the HARFB. The highest hydrogen generation was 10.3 moles H2 (m3 d-1) in reactor R1, and the highest methane yield was 312.0 and 283.9 L CH4 (m3 d-1) in R2 and R3, respectively. The three-stage system showed high efficiency in removing crude glycerol and Chemical Oxygen Demand (COD), with consumptions of 99.9%. In addition, there was a change in the relative abundance of microorganisms amongst R1, R2, and R3 and a considerable decrease in the diversity index in the fermentation reactor (R1). The results have shown the potential of applying HARFB reactors for energy recovery and alternative waste disposal.

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The effect of mixing intensity on the performance and microbial dynamics of a single vertical reactor integrating acidogenic and methanogenic phases in lignocellulosic biomass digestion

Cited by 43 publications

References 26 publications

Impact of mixing intensity and duration on biogas production in an anaerobic digester: a review

Impact of mixing intensity and duration on biogas production in an anaerobic digester: a review

Adaptive Pathways of Microorganisms to Cope With the Shift From P- to N-Limitation in Subtropical Plantations

Bioconversion of Crude Glycerol to Hydrogen and Methane in Three-Stage Horizontal Anaerobic Fixed-Bed Reactors (HARFB)

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