The Effect of miR-539 Regulating TRIAP1 on the Apoptosis, Proliferation, Migration and Invasion of Osteosarcoma Cells

Liu, Huowen; Zhang, Yufeng; Yang, Zhiqiang; Chen, Zhe; Xie, Yuanlong; Peng, Binglong; Cai, Lin

doi:10.21203/rs.3.rs-133262/v1

Cited by 4 publications

(5 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present study identified hsa-miR-205-5p and hsa-miR-539-3p as differentially expressed miRNAs in the lung tissue of SSc-PAH patients, which might help us understand the roles of miRNAs in the pathogenesis of SSc-PAH. Previous studies also indicated that the abnormal hsa-miR-205-5p and hsa-miR-539-3p expressions existed in the various cancers (21,22). These results demonstrated that the two miRNAs may be the potential biomarkers of the early diagnosis, disease evaluation and prognosis judgment in SSc-PAH.…”

Section: Discussionmentioning

confidence: 58%

“…Available at https://dx.doi. org/10.21037/atm- 21 Ethical Statement: All authors are accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration (as revised in 2013).…”

Section: Footnotementioning

confidence: 99%

See 1 more Smart Citation

The expression profiling of microRNA in systemic sclerosis-associated pulmonary arterial hypertension

Huang¹,

Li²,

Liu³

et al. 2021

Ann Transl Med

View full text Add to dashboard Cite

Background: The role of microRNAs (miRNAs) in the pathogenesis of systemic sclerosis-associated pulmonary arterial hypertension (SSc-PAH) remains to be fully elucidated. This study evaluated the expression profile of miRNAs in the lung tissue of patients with SSc-PAH.Methods: Lung tissue samples were collected from 3 SSc-PAH patients and 4 healthy controls. A small RNA high throughput sequence approach was used for screening the differentially expressed miRNAs in the lung tissue samples. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to validate 4 highly significant differentially expressed miRNAs. Gene Ontology and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis for mRNAs were performed using the R package clusterProfiler software.Results: A total of 82 upregulated miRNAs and 35 downregulated miRNAs were detected in the lung tissues of patients with SSc-PAH compared with healthy controls. GO enrichment analysis demonstrated that the upregulated target genes were closely involved in biological processes such as nervous system development, anatomical structure morphogenesis, system development, cellular macromolecule metabolic processes, and cellular processes. The downregulated target genes were involved in the plasma membrane bound cell projection morphogenesis and the regulation of macromolecule metabolic processes. The KEGG enrichment analysis showed that the upregulated genes were associated with important pathways involved in cancer biology, and the target genes of the downregulated miRNAs were involved in axon guidance. High throughput sequencing and qRT-PCR revealed that hsa-miR-205-5p and hsa-miR-539-3p were differentially expressed in SSc-PAH tissue. The target genes of hsa-miR-205-5p and hsa-miR-539-3p, IRF1and ADCYAP1, respectively, were verified using the high throughput dataset GSE48149.Conclusions: miRNAs may play an important role in the pathogenesis of SSc-PAH, and hsa-miR-205-5p and hsa-miR-539-3p may be potential therapeutic targets in patients with SSc-PAH.

show abstract

Section: Discussionmentioning

confidence: 58%

Section: Footnotementioning

confidence: 99%

The expression profiling of microRNA in systemic sclerosis-associated pulmonary arterial hypertension

Huang¹,

Li²,

Liu³

et al. 2021

Ann Transl Med

View full text Add to dashboard Cite

show abstract

“…Previous studies have found that miRNAs play an important role in the pathogenesis of tumors [28][29][30]. Furthermore, miRNAs play a vital role in the occurrence and development of OS [31,32]. Although miR-875-5p is involved in inhibiting tumor progression in a variety of tumors [18,19], the mechanism of action of miR-875-5p in OS has not previously been studied.…”

Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA LINC00909 Induces Epithelial-Mesenchymal Transition and Contributes to Osteosarcoma Tumorigenesis and Metastasis

Liu

Zhang

Shen

et al. 2022

Journal of Oncology

View full text Add to dashboard Cite

Background. Osteosarcoma (OS) is a malignant tumor that is highly metastatic with a high mortality rate. Although mounting evidence suggests that LINC00909 is strongly associated with the malignant progression of various tumors, the exact role of LINC00909 in OS remains unknown. Therefore, the current study was designed to investigate the relationship between LINC00909 and the malignant progression of OS. Methods. LINC00909 expression was measured in OS cell lines and clinical specimens using RT-qPCR assays. The effects of LINC00909 on OS proliferation, invasion, and migration were calculated both in vitro and in vivo. Apart from that, bioinformatics analyses, FISH, RIP, and luciferase reporter assays were carried out to investigate the downstream target of LINC00909. Rescue experiments were also conducted to investigate the potential mechanisms of action of competitive endogenous RNAs (ceRNAs). Results. In this study, we found that LINC00909 was highly expressed in OS cell lines and clinical specimens. In vivo and in vitro experiments demonstrated that LINC00909 induces epithelial-to-mesenchymal transition (EMT) and contributes to OS tumorigenesis and metastasis. FISH, RIP, and luciferase assays indicated that miR-875-5p is a direct target of LINC00909. Moreover, HOXD9 was validated as the downstream target of miR-875-5p in a luciferase reporter assay and western blotting experiments. Rescue experiments revealed that HOXD9 reversed the effect of LV-sh-LINC00909 on OS cells by positively regulating the PI3K/AKT/mTOR signaling pathway. Conclusion. Collectively, LINC00909 induces EMT and contributes to OS tumorigenesis and metastasis through the PI3K/AKT/mTOR pathway by binding to miR-875-5p to upregulate HOXD9 expression. Targeting the LINC00909/miR-875-5p/HOXD9 axis may have potential in treating OS.

show abstract

“…Since miRNAs can inhibit mRNA translation by complementary pairing with the 3'-UTR, thus regulating target gene expression [26,27]. For example, miRNA-30a-3p was found to inhibit GC cell proliferation and migration by targeting APBB2 [28]; miRNA-539 inhibited osteosarcoma cell proliferation, apoptosis, invasion and migration by targeting TRIAP1 [29]. MiRNA-34c-5p is a member of the miRNA-34 family, miRNA-34 family can inhibit tumor progression by promoting apoptosis and blocking the cell cycle [30].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-34c-5p exhibits anticancer properties in gastric cancer by targeting MAP2K1 to inhibit cell proliferation, migration, and invasion

Zhao

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

Purpose. Gastric cancer(GC)is one of the deadliest digestive tract tumors worldwide，existing studies suggest that dysregulated expression of microRNAs (miRNAs) plays an important role in the pathogenesis and progression of GC. This study aimed to investigate the expression, biological function, and downstream mechanism of miR-34c-5p in GC, provide new targets for gastric cancer diagnosis and treatment. Methods. The expression of miR-34c-5p in GC tissues and cell lines was examined by RT-qPCR. Cell wound healing, transwell and cell cloning assays were used to detect the effect of miR-34c-5p on the migration and invasion abilities, respectively, of GC cells. Western blot was performed to detect the expression of related proteins. Bioinformatics analysis was used to predict the binding of MAP2K1 to miR-34c-5p and the targeting relationship was confirmed by dual luciferase reporter assay. Results. The expression level of miR-34c-5p was significantly decreased in GC tissues and cell lines. miR-34c-5p overexpression inhibited migration, invasion, and colony formation of gastric cancer cells, the related protein E-cadherin expression was significantly increased and N-cadherin, vimentin, and PCNA expression were significantly decreased, while miR-34c-5p knockdown exerted the opposite effects. In addition, the targeting relationship between miR-34c-5p and MAP2K1 was predicted and confirmed, and further confirmed by rescue experiments that MAP2K1 alleviated the inhibitory effect of miR-34c-5p in GC. Conclusion. MiR-34c-5p is lowly expressed in GC, and it can target MAP2K1 to exert inhibitory effects on GC proliferation, invasion, and migration. These findings provide a promising biomarker and a potential therapeutic target for gastric cancer.

show abstract

The Effect of miR-539 Regulating TRIAP1 on the Apoptosis, Proliferation, Migration and Invasion of Osteosarcoma Cells

Cited by 4 publications

References 17 publications

The expression profiling of microRNA in systemic sclerosis-associated pulmonary arterial hypertension

The expression profiling of microRNA in systemic sclerosis-associated pulmonary arterial hypertension

Long Noncoding RNA LINC00909 Induces Epithelial-Mesenchymal Transition and Contributes to Osteosarcoma Tumorigenesis and Metastasis

MicroRNA-34c-5p exhibits anticancer properties in gastric cancer by targeting MAP2K1 to inhibit cell proliferation, migration, and invasion

Contact Info

Product

Resources

About