The effect of metal mixture composition on toxicity to C. elegans at individual and population levels

Moyson, Sofie; Town, Raewyn M.; Vissenberg, Kris; Blust, Ronny

doi:10.1371/journal.pone.0218929

Cited by 19 publications

(11 citation statements)

References 57 publications

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“…32 On the other hand, population size could be considered a sensitive end point for the toxicity assessment of heavy metals. 33 The population decline observed in this study was closely related to the function of the reproductive system of the nematodes. This implies that chronic exposure to low doses of MeHg (above 100 nM) may have induced significant reproductive toxicity in C. elegans.…”

Section: Resultsmentioning

confidence: 59%

“…Adverse effects of pollutants on the offspring through the parent have a more significant impact on the population level . On the other hand, population size could be considered a sensitive end point for the toxicity assessment of heavy metals . The population decline observed in this study was closely related to the function of the reproductive system of the nematodes.…”

Section: Resultsmentioning

confidence: 93%

See 1 more Smart Citation

Ecotoxicity Risk of Low-Dose Methylmercury Exposure to Caenorhabditis elegans: Multigenerational Toxicity and Population Discrepancy

et al. 2021

Chem. Res. Toxicol.

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Methylmercury (MeHg) is a common organic form of mercury in water, which has been linked to several forms of biological toxicity. However, studies on the ecotoxicity risk of long-term exposure to low-dose MeHg are insufficient for the assessment of environmental safety. In the present study, the effects of MeHg on multiple generations (P0–F3) and population of Caenorhabditis elegans were investigated under long-term, low-dose exposure. We investigated the multigenerational toxicity of MeHg by analyzing reproductive and developmental indicators. According to our results, exposure to 100 nM MeHg had little effect on the parental generation (P0) but caused serious reproductive toxicity in the offspring (F1–F3), and the effect of MeHg was aggravated with each passing generation. The genes related to apoptosis and DNA damage were upregulated in the F3 generation. Pearson correlation analysis showed that the changes in these genes were closely related to the apoptosis of gonadal cells. Furthermore, chronic exposure to MeHg (from 100 to 1000 nM group) caused a sharp decline in population size and triggered the “bag of worms” phenotype. Genes related to vulvar development were downregulated in the F3 generation after treatment with 100 nM MeHg. These data suggest that long-term low-dose MeHg exposure adversely affected C. elegans and its offspring and triggered multigenerational toxicity and population discrepancy.

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Section: Resultsmentioning

confidence: 59%

Section: Resultsmentioning

confidence: 93%

Ecotoxicity Risk of Low-Dose Methylmercury Exposure to Caenorhabditis elegans: Multigenerational Toxicity and Population Discrepancy

et al. 2021

Chem. Res. Toxicol.

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“…Moreover, although the chosen doses were very similar to those used by Moyson et al. (2019), in our study we did not use a mixture of metals, such as Zn, Cd, and Cu, for long‐term exposure. In this study, we aimed to determine if organic products that contained copper would be safe to consume.…”

Section: Discussionmentioning

confidence: 93%

Copper uses in organic production are safe to the nervous system of Caenorhabditis elegans?

Philippsen

Tamagno

Vanin

et al. 2021

Environmental Quality Mgmt

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The Bordeaux mixture, fungicides composed of copper sulfate, is commonly used in organic production. This use has been questioned owing to the impact of copper accumulation in the environment, which can affect living organisms negatively. Here, was analyzed the presence of copper levels in soils and in orange and grape juices that received Bordeaux mixture fungicide treatments. Furthermore, we evaluated copper toxicity in Caenorhabditis elegans nematodes by assessing some of their biochemical and behavioral parameters after acute and chronic copper exposure. The metal exposure in vivo was performed at concentrations of 0.05, 0.1, 0.3, and 0.7 mg/L, compared with a control group that received ultrapure water. The results showed that copper was present in the sampled soils at higher levels than in the control area soil. Copper was detected in an average value of 0.25 mg/L in orange juice and 0.69 mg/L in grape juice. The acute and chronic exposure conditions showed behavioral changes and an activation of the acetylcholinesterase enzyme. However, chronic exposure to low copper concentrations led to inhibition of this enzyme. Thus, the copper concentrations detected in the fruit juices acted as modulators of the cholinergic system and modified the behavioral parameters in C. elegans.

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“…The entire estimated dose–response curve for the mixture may be compared to the predicted dose–response curve under the assumption of independent action, obtained from Eqns () or (), either through visual inspection or by means of statistical tests such as two‐sample t ‐tests or non‐parametric equivalents (comparing fitted and predicted values dose by dose) 30 . These statistical methods assume independence between fitted and predicted values and, therefore, they are not entirely appropriate.…”

Section: Methodsmentioning

confidence: 99%

How to use statistics to claim antagonism and synergism from binary mixture experiments

Ritz

Streibig²,

Kniss

2021

Pest Management Science

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We review statistical approaches applicable for the analysis of data from binary mixture experiments, which are commonly used in pesticide science for evaluating antagonistic or synergistic effects. Specifically, two different situations are reviewed, one where every pesticide is only available at a single dose level and a mixture simply combines these doses, and one where the pesticides and their mixture are used at increasing doses. The former corresponds to using factorial designs whereas the latter corresponds to fixed‐ratio designs. We consider dose addition and independent action as references for lack of antagonistic and synergistic effects. Data from factorial designs should be analyzed using two‐way analysis of variance models whereas data from fixed‐ratio designs should be analyzed using non‐linear dose–response analysis. In most cases, independent action seems the more natural choice for factorial designs. In contrast, dose addition is more appropriate for fixed‐ratio designs although dose addition is not equally compatible with all types of dose–response data. Fixed‐ratio designs should be preferred as they allow validation of the assumed dose–response relationship and, consequently, provide much stronger claims about antagonistic and synergistic effects than factorial designs. Finally, it should be noted that, in any case, simple ways of summarizing pesticide mixture effects may come at the price of more or less restrictive modeling assumptions. © 2021 Society of Chemical Industry.

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The effect of metal mixture composition on toxicity to C. elegans at individual and population levels

Cited by 19 publications

References 57 publications

Ecotoxicity Risk of Low-Dose Methylmercury Exposure to Caenorhabditis elegans: Multigenerational Toxicity and Population Discrepancy

Ecotoxicity Risk of Low-Dose Methylmercury Exposure to Caenorhabditis elegans: Multigenerational Toxicity and Population Discrepancy

Copper uses in organic production are safe to the nervous system of Caenorhabditis elegans?

How to use statistics to claim antagonism and synergism from binary mixture experiments

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