The effect of metabolites of testosterone on the development of fertilizing ability by spermatozoa in the epididymis of castrated hamsters

Cm, Lubicz-Nawrocki

doi:10.1002/jez.1401970110

Cited by 13 publications

(5 citation statements)

References 25 publications

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“…The explanation for this effect appears to lie in the relative capacity of the glands to convert the diol to 5a-dihydrotestosterone. A similar situation may exist in the hamster epididymis where apparently 5a-androstane-3a,17ß-diol is a very 'potent' androgen (Lubicz-Nawrocki, 1976).…”

Section: Discussionmentioning

confidence: 74%

METABOLISM OF TESTOSTERONE AND 5α-Reduced ANDROGENS BY THE RABBIT PROSTATE AND EPIDIDYMIS: STUDIES IN VITRO AND IN VIVO

Booth¹,

Jones²

1979

Journal of Endocrinology

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The metabolism of radioactively labelled testosterone, 5\g=a\-dihydrotestosterone, 5\g=a\-androstane-3\g=a\,17\g=b\-diol and 5\g=a\-androstane-3\g=b\,17\g=b\-diolby the rabbit epididymis and prostate has been investigated in vitro and in vivo. In vitro, the rate of conversion of testosterone to 5\ g=a\ \ x =r eq-\ reduced androgens was low in both glands. Varying the nature of the tissue preparation, age of animal or incubation medium did not improve the situation substantially. However, the rabbit prostate and epididymis metabolized 5\g=a\-reduced androgens readily. The prostate was particularly efficient at interconverting 5\g=a\-androstane-3\g=a\,17\g=b\-diol and 5\g=a\-dihydrotestosterone, whereas the capacity of the epididymis to carry out this step was much lower. Small amounts of 5\g=a\-androstane-3,17-dione and androsterone were also identified. Both glands interconverted 5\g=a\-dihydrotestosterone and 5\g=a\-androstane-3\g=b\,17\g=b\-diol to a comparable degree.Following the intravenous injection of 3H-labelled testosterone, significant levels of 3H-labelled 5\g=a\-dihydrotestosterone were found in the prostate and epididymis within 30 min. Furthermore, 5\g=a\-androstane-3\g=a\,17\g=b\-diol was detected in both glands. In blood plasma, the ratio of radioactively labelled testosterone : 5\g=a\-dihydrotestosterone was 2 : 1, i.e. similar to that for endogenous steroids. The intravenous injection of 3H-labelled 5\g=a\-androstane-3\g=a\\x=req-\ 17\g=b\-diolgave rise to much higher amounts of 5\g=a\-dihydrotestosterone in the prostate than in the epididymis, whereas the reverse was found for the levels of unmetabolized diol.The results indicate that the prostate and epididymis of the adult rabbit differ in their capacity to metabolize 5\g=a\-reduced androgens and that both glands depend to a large extent on the relatively high levels of 5\g=a\-dihydrotestosterone and 5\g=a\-androstanediols present in the peripheral circulation, rather than the metabolism of testosterone in situ.

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Section: Discussionmentioning

confidence: 74%

METABOLISM OF TESTOSTERONE AND 5α-Reduced ANDROGENS BY THE RABBIT PROSTATE AND EPIDIDYMIS: STUDIES IN VITRO AND IN VIVO

Booth¹,

Jones²

1979

Journal of Endocrinology

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“…It is generally recognized that the cauda epididymidis of eutherian mammals is adapted to store spermatozoa (Glover & Nicander, 1971;Bedford, 1979) and that the storage function is androgen dependent (Dyson & Orgebin-Crist, 1973;Lubicz-Nawrocki & Glover, 1973a, b;Orgebin-Crist, 1975;Lubicz-Nawrocki, 1976). For example, it has been shown, using ligatures to retain spermato¬ zoa in the cauda epididymidis, that spermatozoa maintain their capacity to fertilize an ovum for 25 days in the mouse , 21 days in the rat (White, 1932(White, , 1933, 20-30 days in the guinea-pig (Young, 1929), 20-25 days in the hamster and 28-35 days in the rabbit (Hammond & Asdell, 1926;Paufler & Foote, 1968;Tesh & Glover, 1969).…”

Section: Introductionmentioning

confidence: 98%

Role of androgens in survival of spermatozoa in epididymis of tammar wallaby (Macropus eugenii)

Chaturapanich¹,

Jones²,

Clulow³

1992

Reproduction

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Studies of undiluted micropuncture samples of luminal fluid from the cauda epididymidis of the tammar indicated that spermatozoa are immotile in situ and spontaneously activate during collection or subsequent incubation in vitro. The suppression of sperm motility was related to the androgen status of the tammars and when this was increased by the use of Silastic implants of testosterone propionate, the spontaneous activation of samples was delayed for up to 2 h during incubation in vitro. Spermatozoa survived for up to 9 weeks when isolated in the cauda epididymidis between ligatures around the ductus. However, even after isolation for 3 weeks their viability was reduced compared with samples from the contralateral, unligated duct. Isolation of a length of ductus between ligatures also reduced the concentration of spermatozoa in the lumen of the duct and reduced the concentration of some proteins in the epididymal plasma. However, it did not affect the electrophoretic pattern of detergent extracts of spermatozoa. A study of the effects of orchidectomy and testosterone therapy indicated that sperm survival in the epididymis is androgen dependent. Orchidectomy reduced the concentration of spermatozoa in the luminal fluid and the volume of luminal fluid, and resulted in an increase in the concentration and a change in the electrophoretic pattern of protein in the fluid. The effects of orchidectomy were reduced or prevented by testosterone therapy. It is concluded that the cauda epididymidis of the tammar is at least as well adapted for sperm storage as it is in the eutherian mammals that have been studied.

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“…Several androgens can provide this, notably testosterone (Blaquier, Cameo & Burgos, 1972;Dyson & Orgebin-Crist, 1973), 17/?-hydroxy-5oc-androstan-3-one (5a-dihydrotestosterone; DHT) (Orgebin-Crist, Jahad & Hoffman, 1976) and 5a-androstane-3a,17/?-diol (Lubicz-Nawrocki, 1973,1976. The latter two are produced by the epididymis from testosterone (Inano, Machino & Tamaoki, 1969) by a 5a-reductase alone and a 5a-reductase in conjunction with a 3oc-hydroxysteroid oxidoreductase respect¬ ively (Robaire, Ewing, Zirkin & Irby, 1977).…”

Section: Introductionmentioning

confidence: 99%

Age-Related Changes in the Metabolism of [3h]testosterone in Vitro by the Epididymis of the Immature Rat

Foldesy¹,

Leathem²

1981

Journal of Endocrinology

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We examined the production in vitro of 5 alpha-reduced metabolites from testosterone by the rat epididymis during pubertal maturation. Minced caput and cauda epididymides from 30-,45-, and 55-day-old rats were incubated with [3H] testosterone for 2h. Analysis of the radioactive metabolites revealed both similarities and differences in the metabolic patterns compared to those reported for adult rats. As in adults, 5 alpha-dihydrotestosterone was the most abundant metabolite produced by both epididymal segments at all three ages, and it was formed in larger quantities in the caput epididymidis than in the cauda. However, [3H] testosterone metabolism by the epididymis of the immature rat was characterized by a lower formation of 5 alpha-androstane-3 alpha,17 beta-diol and higher production of 5 alpha-androstane-3,17-dione than in adults. Production of these two metabolites by the caput region increased and decreased respectively, toward adult levels, with increasing age. In addition, the amount of [3H] testosterone metabolized was higher with tissues from prepubertal rats (30 days of age) than with those from rats 55 days of age. These data suggest that testosterone metabolism in the caput begins to change to that of the adult during the period of pubertal maturation but apparently not until later in the cauda epididymidis.

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The effect of metabolites of testosterone on the development of fertilizing ability by spermatozoa in the epididymis of castrated hamsters

Cited by 13 publications

References 25 publications

METABOLISM OF TESTOSTERONE AND 5α-Reduced ANDROGENS BY THE RABBIT PROSTATE AND EPIDIDYMIS: STUDIES IN VITRO AND IN VIVO

METABOLISM OF TESTOSTERONE AND 5α-Reduced ANDROGENS BY THE RABBIT PROSTATE AND EPIDIDYMIS: STUDIES IN VITRO AND IN VIVO

Role of androgens in survival of spermatozoa in epididymis of tammar wallaby (Macropus eugenii)

Age-Related Changes in the Metabolism of [3h]testosterone in Vitro by the Epididymis of the Immature Rat

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