The effect of melatonin on bovine<i>in vitro</i>embryo development

Tsantarliotou, Maria; Attanasio, Laura B.; Rosa, A. De; Boccia, Lorenzo; Pellerano, Graciela; Gasparrini, B.

doi:10.4081/ijas.2007.1s.488

Cited by 20 publications

(19 citation statements)

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“…Melatonin during IVM increases the nuclear maturation and blastocyst rates in porcine (Kang et al, 2009) and in buffalo (Manjunatha et al, 2009). On the other hand, in agreement with our results, Tsantarioutou et al (2007) observed no differences on bovine cleavage and blastocyst rates when melatonin was added to IVM medium. Those authors did not study the nuclear maturation rate.…”

Section: Resultssupporting

confidence: 82%

“…The protective role of free radical scavengers in maturation culture medium has been documented (Tsantarioutou et al, 2007;Kang et al, 2009;Manjunatha et al, 2009). Melatonin acts as a potent scavenger of free radicals (Tan et al, 2007) and a direct gonadal response to melatonin has been also reported, including stimulation of progesterone (Adriaens et al, 2006) and estradiol synthesis (Sirotkin and Schaeffer, 1997), and improvement of oocyte quality and maturation (Chattorag et al, 2005;Kang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Takada

Martins

Mingoti

et al. 2010

Sci. agric. (Piracicaba, Braz.)

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Melatonin (MEL) acts as a powerful scavenger of free radicals and direct gonadal responses to melatonin have been reported in the literature. Few studies, however, have evaluated the effect of MEL during in vitro maturation (IVM) on bovine embryos. This study tested the addition of MEL to maturation medium (MM) with no gonadotropins on nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos. Cumulus-oocyte complexes were aspirated from abattoir ovaries and cultured in MM (TCM-199 medium supplemented with 10% fetal calf serum -FCS) at 39°C and 5% CO 2 in air. After 24 hours of culture in MM with 0.5 μg mL -1 FSH and 5.0 μg mL -1 LH; 10 -9 M MEL) or 10 -9 M MEL, 0.5 μg mL -1 FSH and 5.0 μg mL -1 LH, the oocytes were stained with Hoechst 33342 to evaluate nuclear maturation rate. After in vitro fertilization and embryo culture, development rates were evaluated and the blastocysts were assessed for DNA damage by Comet assay. There was no effect of melatonin added to the MM, alone or in combination with gonadotropins, on nuclear maturation, cleavage and blastocyst rates. These rates ranged between 88% to 90%, 85% to 88% and 42% to 46%, respectively. The extent of DNA damage in embryos was also not affected by MEL supplementation during IVM. The addition of 10 -9 M MEL to the MM failed to improve nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos, but was able to properly substitute for gonadotropins during IVM. Key words: comet assay, embryos, gonadotropins, in vitro fertilization, melatonin Melatonina no meio de maturação não melhorou as taxas de maturação dos ovócitos, de desenvolvimento embrionário e a fragmentação do DNA dos embriões bovinos RESUMO: Melatonin (MEL) atua como um potente redutor de radicais livres. Efeito direto da MEL na função gonadal também foi observado. Existem poucos estudos relacionados ao efeito da MEL durante a maturação no desenvolvimento embrionário in vitro. Avaliou-se a adição de MEL no meio de maturação (sem gonadotrofinas) nas taxas de maturação nuclear e de desenvolvimento embrionário e na incidência de fragmentação do DNA nos embriões produzidos in vitro. Complexos cumulus-ovócitos foram aspirados de ovários provenientes de matadouros e cultivados em meio de maturação (Meio TCM-199 suplementado com 10% de soro fetal bovino) a 39°C e 5% CO 2 em ar. Após 24 horas de cultivo em meio de maturação com 0,5 μg mL -1 de FSH e 5,0 μg mL -1 de LH; 10 -9 M de MEL ou 10 -9 M de MEL, 0,5 μg mL -1 de FSH e 5,0 μg mL -1 de LH, os ovócitos foram corados com Hoechst 33342 para avaliação da taxa de maturação nuclear. A fragmentação do DNA dos blastocistos foi avaliada pela técnica do Cometa. Não houve efeito da MEL adicionada ao meio de maturação, com ou sem gonadotrofinas, nas taxas de maturação nuclear, clivagem e blastocistos. Os percentuais variaram entre 88% a 90%, 85% a 88% e 42% a 46%, respectivamente. O grau de fragmentação do DNA também não foi afetado pela adição de MEL ao meio de matur...

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Section: Resultssupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Takada

Martins

Mingoti

et al. 2010

Sci. agric. (Piracicaba, Braz.)

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“…() and Tsantarliotou et al. () tested the melatonin effect with different treatment strategies and did not find any consequence on the development rates of the in vitro ‐produced bovine embryos. Recently, Takada et al.…”

Section: Discussionmentioning

confidence: 99%

Beneficial Effect of Melatonin on Blastocyst In Vitro Production from Heat‐Stressed Bovine Oocytes

Cebrián-Serrano

Salvador

Raga

et al. 2013

Reprod Domestic Animals

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Melatonin may play an important role in protecting gametes and embryos from the potential harmful effects of oxidative stress. In this study, we first examined two different heat stress (HS) treatments for in vitro oocyte maturation (Experiment 1: 38.5 vs 41.0°C, during the first 20 h; Experiment 2: 38.5 vs 41.5°C, during the entire period) on bovine oocyte maturation and embryo development. Second, we tested different melatonin concentrations added to the maturation and culture medium (Experiment 3: 0, 10(-12) , 10(-9) , 10(-4) m; Experiment 4: 0, 10(-3) m), both with and without HS (38.5 or 41.5°C, respectively). In Experiment 1, the HS treatment resulted in a lower maturation rate and number of cells/blastocyst (C/B) and a higher blastocyst rate than that in the control group. In Experiment 2, oocytes/embryos from heat-stressed oocytes (HSO) had a lower maturation, cleavage and blastocyst rates, as well as a lower C/B compared with the control. In Experiment 3, in HSO groups, 10(-4) m melatonin resulted in an increased blastocyst rate compared with 0 m melatonin, with a similar blastocyst rate to the non-HSO without melatonin. Melatonin did not have any effect in embryos from non-HSO groups compared with the control. In Experiment 4, 10(-3) m melatonin produced lower cleavage and blastocyst rates in HSO and lower blastocyst rate in non-HSO when compared to melatonin-untreated oocytes/embryos. In conclusion, 10(-4) m melatonin was found to alleviate bovine oocytes from the harmful effects of HS.

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“…In agreement with our results, Papis et al (2007) and Tsantarliotou et al (2010) 2009), pig (Choi et al, 2008;Kang et al, 2009;Shi et al, 2009) andmice (Ishizuka et al, 2000).…”

Section: Discussionsupporting

confidence: 81%

Factors affecting the in vitro embryo production in cattle associated to ovum pick up sistem

Serrano¹

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Esta tesis ha sido propuesta para Mención Internacional en el título de Doctor en virtud de la siguiente estancia de investigación en el extranjero:• Institute of farm animal genetic, Friedrich-Loeffler Institute, Mariensee, Alemania.Supervisor: Dr. Detlef Rath.Periodo de duración: del 4 de mayo al 15 de octubre de 2009. AGRADECIMIENTOSLas tesis doctorales tienen una cosa en común que las hace épicas; buena parte del camino debe recorrerse en solitario. Sin embargo, sin la ayuda y la hospitalidad de las personas que vamos encontrando por el largo y sinuoso viaje iniciático que supone una tesis doctoral, nos hariamos viejos sin llegar lejos. Sirvan estas líneas para mostrar mi agradecimiento a todos los compañeros y amigos que me han ayudado y han hecho que esta tesis fuera posible.En primer lugar, a mis directores de tesis Miguel Ángel Silvestre y Ignacio Salvador, por darme la posibilidad de trabajar y aprender a su lado y de progresar en el mundo de la investigación.A mis compañeros de laboratorio, Elena Megual, Empar Raga, Empar García-Rosselló, Richard Lopera, Javier Ahumada, Manuel Francisco Simao y Ángel Picó, por su inestimable contribución durante la realización de los experimentos.Al Profesor Detlef Rath, por haberme acogido desinteresadamente en su grupo de investigación y haber puesto a mi disposición los medios necesarios para aprender nuevas técnicas y realizar experimentos, y al Profesor Salvador Ruiz y a los compañeros del Institut für Nutztiergenetik que me ayudaron durante mi estancia en Mariensee, especialmente a Klaus-GerdHadeler, Javier Zaraza y Miguel Velázquez.Al Profesor Jesus Yáñiz y al Profesor Fernando López-Gatius, por su valiosa asistencia técnica en los primeros pasos de esta tesis.A la Asociación Frisona Valenciana y a la Granja S.A.T. San Ramón, por el apoyo prestado durante la gestación y el desarrollo de este proyecto.A los compañeros y amigos del CITA, por su contribución directa/o indirecta a este trabajo y por haber compartidos tantos buenos momentos.Por último, a mis amigos y a mi familia, en especial a mis padres, por su apoyo, ánimo y estima incondicional. Cuando emprendas el viaje hacia

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The effect of melatonin on bovinein vitroembryo development

Cited by 20 publications

References 5 publications

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos

Beneficial Effect of Melatonin on Blastocyst In Vitro Production from Heat‐Stressed Bovine Oocytes

Factors affecting the in vitro embryo production in cattle associated to ovum pick up sistem

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