The effect of magnetic nanoparticles of Fe3O4 on immune function in normal ICR mice

Abstract

Abstract:We investigated the effect of magnetic nanoparticles of Fe 3 O 4 (Fe 3 O 4 -MNPs) on the mice immune system. Imprinting control region (ICR) mice were assigned randomly into four groups and treated with normal saline or low, medium, or high doses of Fe 3 O 4 -MNPs, respectively. After intravenous administration of Fe 3 O 4 -MNPs for 72 hours, the peripheral T cells and the induction of primary immune responses in mice were investigated by flow cytometry and determined using enzyme-linked immunosorbent assay, respectively. The results showed that the ratio of spleen to body weight was not different between the experimental groups and control group (P . 0.05). The lymphocyte transformation rates in the suspension of spleen were higher in low-dose group than those in the control group (P , 0.05), while the proliferation of splenocytes was low in the medium and high groups when compared to the control group (P , 0.05). In peripheral blood, both the proportions of subset CD4+ and CD8 + T lymphocytes in the low-dose group were higher than those in the control group, whereas there was no difference in the number of CD4 + T cells between the medium-and low-dose groups. Interestingly, the Nanotechnology offers an efficient alternative for cancer diagnostics and tumor target treatment due to the unique properties of nanostructures, such as large surface-to-volume ratio, porous structure, embedded effect, and size effect, which have been recognized as offering potential promising applications in biomedical engineering. Much effort has been extended to the development of novel nanocomposites and biomaterials for DNA detection, 1 intracellular labeling, 2 drug carrier, 3 cancer targeting, 4 imaging, 5 and so on. Therefore, magnetic nanoparticles of Fe 3 O 4 (Fe 3 O 4 -MNPs) as a kind of biocompatible nanomaterial which is feasible to characterize and easily functionalize, may offer an exciting development toward developing an effective drug delivery system while biocompatible superparamagnetic particles like magnetite could be utilized in tissue-specific release of therapeutic agents and magnetic field assisted radionuclide therapy. [6][7][8][9] As a novel material, though we have already proved that Materials and methods experimental agentsExperimental agents were sourced from the following locations: RPMI1640 (Gibco Chemical Co., Carlsbad, CA, USA); Anti-CD3 (PE-Cy5), Anti-CD4, Anti-CD8 ( Pharmingen, San Diego, CA); Calf serum (Gibco Chemical Co); Enzyme-linked immunosorbent assay kit (Gibco, CA, USA); Con A (Sigma Chemical Co., St Louis, MO, USA). Preparation of Fe 3 O 4 -MNPsBased on our previous studies, 10-11 the synthesis of Fe 3 O 4 -MNPs was prepared by the electrochemical deposition under oxidizing conditions. Before being applied in the present experiment, the magnetite nanoparticles were well-distributed in RPMI-1640 medium freshly added with 10% heated-inactivated fetal bovine serum (FBS) using ultrasound treatment in order to obtain Fe 3 O 4 -MNPs colloidal suspension. Animals and animal careFemale an...

Superparamagnetic iron oxide nanoparticles: magnetic nanoplatforms as drug carriers

“…135 Further, SPIONs can alter immune function in a dose-dependent manner, as described by Chen et al in an imprinting control region mouse model. 10 In a study conducted by Mahmoudi et al, the main reason found for SPION-induced toxicity was formation of gas vesicles as a result of local alteration in ionic equilibrium and protein function. Mahmoudi et al developed a modified method for assessing cytotoxicity associated with SPIONs to establish a better correlation between in vitro and in vivo studies.…”

“…Much research has been carried out to evaluate the biocompatibility of these magnetic nanoparticles and their possible adverse interactions with cellular and subcellular structures. 9,10 In this review, we discuss their desirable characteristics, including shape, hydrodynamic volume, surface charge and colloidal stability, various methods used for preparation of these nanoparticles, and their role in the biomedical sciences. Furthermore, limitations of SPIONs as drug delivery agents and their toxicity are discussed in detail.…”

Iron oxide nanoparticles suppressed T helper 1 cell-mediated immunity in a murine model of delayed-type hypersensitivity

“…Although iron oxide nanoparticles have been reported to affect the functionality of T cells and antigen-presenting cells, [12][13][14][15][16]18,19,23,24 evidence pertaining to their effects on Th1 cell-mediated immunity in vivo remains mostly unknown. It was previously reported that systemic administration of ovalbumin-sensitized mice with iron oxide nanoparticles suppresses, the serum production of ovalbumin-specific immunoglobulin G1 and immunoglobulin G2a, and the expression of both IFN-γ and IL-4 by splenocytes.…”

“…Oral and intravenous administrations of iron oxide nanoparticles alter T cell cellularity in normal nonsensitized mice. 16,17 It has been reported that serum levels of interleukin-2 (IL-2), IL-10, and interferon-γ (IFN-γ) are elevated in mice intravenously administered with iron oxide nanoparticles. 16 Moreover, it was recently reported that exposure to iron oxide nano particles suppresses humoral immunity and influences antigenspecific T cell reactivity.…”

“…16,17 It has been reported that serum levels of interleukin-2 (IL-2), IL-10, and interferon-γ (IFN-γ) are elevated in mice intravenously administered with iron oxide nanoparticles. 16 Moreover, it was recently reported that exposure to iron oxide nano particles suppresses humoral immunity and influences antigenspecific T cell reactivity. 18,19 Collectively, these results clearly demonstrate that the functionality of macrophages and T cells is modulated by iron oxide nanoparticles upon in vitro and in vivo exposure.…”