The effect of LyPRP/collagen composite hydrogel on osteogenic differentiation of rBMSCs

Abstract

Although platelet-rich plasma (PRP) plays a significant role in the orthopedic clinical application, it still faces two major problems, namely, uncontrollable factors release, frequent preparation and extraction processes as well as the inconvenient form of usage. To overcome these shortcomings, freeze-dried PRP (LyPRP) was encapsulated into bioactive Col I hydrogel to induce osteogenic differentiation of rabbit bone marrow mesenchymal stem cells (rBMSCs). And PRP/Col І composite hydrogel was prepared as a control. Compared with Col І hydrogel, the introduction of platelets significantly improved the mechanical properties of hydrogels. Meanwhile, platelets were evenly distributed in the composite hydrogels network. The sustainable release of related factors in the composite hydrogels could last for more than 14 days to maintain its long-term biological activity. Further cell experiments confirmed that PRP and LyPRP could effectively alleviate the contraction of collagen hydrogel in vitro, and promote the adhesion, proliferation and osteogenesis differentiation of rBMSCs. The results of osteogenic gene expression indicated that the 10% LyPRP/Col І composite hydrogel could facilitate the early expression of BMP-2 and late osteogenic associated protein formation with higher expression of alkaline phosphatase and Osteocalcin (OCN). These results might provide new insights for the clinical application of 10% LyPRP/Col І composite hydrogel as practical bone repair injection.

Evaluation of the immunomodulatory effects of cobalt, copper and magnesium ions in a pro inflammatory environment

“…These results are in accordance with a previous study in which cell culture media supplemented with 0.4 and 20 mM Mg 2+ promoted an increase in the expression of CD206 and IL-10, while decreasing pro-inflammatory markers such as CCR7 and TNF-α 23 . Moreover, the release of magnesium ions from certain biomaterials such as magnesium microparticles has also promoted macrophage polarization towards an M2 phenotype 39 . In these studies, released concentrations ranging from 2 to 11.14 mM reduced pro-inflammatory cytokine expression.…”

Enlargement, Reduction, and Even Reversal of Relative Migration Speeds of Endothelial and Smooth Muscle Cells on Biomaterials Simply by Adjusting RGD Nanospacing

“…Properties of interfaces and surfaces of implanted biomaterials, such as biochemical composition, ligand organization, stiffness, viscoelasticity, topology, and even nanoscale cues, have been proved effective for triggering cell responses. − Among these, RGD (Arg–Gly–Asp) peptides can enhance cell–substrate interactions and can also regulate cell behaviors like adhesion, migration, proliferation, differentiation, dedifferentiation, etc. − In the present work, RGD peptides were used for surface modification to explore whether its nanospacing can regulate relative cell migration speeds among different cell types. ECs and SMCs were chosen as representative cell types.…”

Exosome-functionalized magnesium-organic framework-based scaffolds with osteogenic, angiogenic and anti-inflammatory properties for accelerated bone regeneration

“…In terms of osteogenic differentiation, we explored the effects of different composite scaffolds on the expression of osteogenic proteins ( Fig. 3 E); ALP and Runx2 are important early stage markers in osteogenic differentiation [ 48 , 49 ] and OCN appears in late osteogenic differentiation periods [ 50 ]. We seeded hBMSCs on different composite scaffolds, and after 14 days culturing, the PLGA/Mg-GA 2 scaffold showed significantly upregulated ALP, Runx2, and OCN expression when compared with PLGA/Mg-GA 1 , PLGA/Mg-GA 3 , and PLGA groups.…”