The Effect of Lycopene on DNA Damage and Repair in Fluoride-Treated NRK-52E Cell Line

Çetin, Sedat; Usta, Ayşe; Yüksek, Veysel

doi:10.1007/s12011-020-02288-4

Cited by 8 publications

(3 citation statements)

References 34 publications

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“…More precisely, several enzymes require divalent metal ions as cofactors such as magnesium, calcium, zinc and selenium which have a strong affinity with fluoride, and thus can induce oxidative stress resulting from an inhibitory interaction of these enzymes 13 , 48 , 49 . For that, recent studies focused on supplementation of animals with antioxidants such as gallic acid 50 , quercetin 51 , vitamins 7 , 46 , 52 and medicinal plants 6 , 19 , 26 . In this study, Spirulina platensis showed an ameliorative effect against oxidative stress in fluorotic sheep species.…”

Section: Discussionmentioning

confidence: 99%

An interventional clinical trial investigating the effects of Spirulina platensis on dental fluorosis and antioxidant system in lambs reared in endemic areas

Rahim,

Sibaoueih,

Essamadi

et al. 2023

Sci Rep

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This study aimed to evaluate the protective effect of Spirulina platensis primary against dental fluorosis and secondary against oxidative stress in lambs reared in endemic fluorosis areas. Forty-eight lambs aged 5 months were divided into four equal groups (each one including 6 males and 6 females). Groups I and II served as controls belonging respectively to fluorosis-free (Settat) and endemic fluorosis (El Fokra) areas, while the other two Groups III and IV (belonging to El Fokra) received respectively a fixed daily intake of 250 and 500 mg/kg bodyweight (BW) of Spirulina platensis. The experiment was carried out for 13 months until the adult incisors appeared for all animals. According to the Dean’s Fluorosis Index (DFI), 500 mg/kg BW/day of Spirulina platensis (Group IV) protected against dental fluorosis. Moreover, in both male and female lambs, this dose significantly (p < 0.0001) reduced the plasmatic levels of fluoride, proteins, GSH, and MDA compared to the Group II. Furthermore, enzymatic activities of catalase and SOD increased significantly (p < 0.0001) in male and female lambs of the Group IV as compared to Group II. In conclusion, our findings support the potential use of Spirulina platensis as a valuable solution for addressing fluorosis in sheep, warranting further clinical trials.

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Section: Discussionmentioning

confidence: 99%

An interventional clinical trial investigating the effects of Spirulina platensis on dental fluorosis and antioxidant system in lambs reared in endemic areas

Rahim,

Sibaoueih,

Essamadi

et al. 2023

Sci Rep

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“…Likewise, lycopene administration protected indomethacin‐induced DNA damage in rats (Boyacioglu et al, 2016 ) and ochratoxin‐A‐inducing DNA damage in hepatic and kidney tissues (Aydin et al, 2013 ). Other studies also include protection against gamma‐radiation‐inducing DNA damage in hepatocytes (Srinivasan et al, 2007 ), sodium fluoride‐inducing DNA damage in NRK‐52E kidney cells (Çetin et al, 2021 ), and deltamethrin‐inducing DNA damage in thyroid cells (Abdul‐Hamid & Salah, 2013 ). From the literature, it is clear that even though lycopene has higher potency in DNA protection, there is a lack of sufficient evidence of the DNA‐damage preventive mechanisms of lycopene, particularly in humans.…”

Section: Potentials Of Lycopene Against Aging Biomarkers and Aging‐re...mentioning

confidence: 99%

Pharmacological potentials of lycopene against aging and aging‐related disorders: A review

Abir

Mahamud

Tonny

et al. 2023

Food Science & Nutrition

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Aging and aging‐related chronic disorders are one of the principal causes of death worldwide. The prevalence of these disorders is increasing gradually and globally. Considering this unwavering acceleration of the global burden, seeking alternatives to traditional medication to prevent the risk of aging disorders is needed. Among them, lycopene, a carotenoid, is abundant in many fruits and vegetables, including tomatoes, grapefruits, and watermelons, and it has a unique chemical structure to be a potent antioxidant compound. This nutraceutical also possesses several anti‐aging actions, including combating aging biomarkers and ameliorating several chronic disorders. However, no systematic evaluation has yet been carried out that can comprehensively elucidate the effectiveness of lycopene in halting the course of aging and the emergence of chronic diseases linked to aging. This review, therefore, incorporates previous pre‐clinical, clinical, and epidemiological studies on lycopene to understand its potency in treating aging disorders and its role as a mimic of caloric restriction. Lycopene‐rich foods are found to prevent or attenuate aging disorders in various research. Based on the evidence, this review suggests the clinical application of lycopene to improve human health and alleviate the prevalence of aging and aging disorders.

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“…[ 3 ] The single‐cell gel electrophoresis or “comet assay” method is a fast and sensitive fluorescent microscopic method utilized to detect DNA damage on a cellular level and determine the extent of this damage. [ 4 ]…”

Section: Introductionmentioning

confidence: 99%

Lycopene prevents cell death in NRK‐52E cells by inhibition of high glucose‐activated DNA damage and apoptotic, autophagic, and necrotic pathways

Usta,

Yüksek,

Çetin

et al. 2024

J Biochem & Molecular Tox

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This study aims to investigate the effects of lycopene on apoptotic, autophagic, and necrotic pathways, oxidative status, and DNA damage in diabetic nephropathy at the molecular level. The sample of the study includes seven groups: lycopene (L), high glucose (G), high glucose + lycopene (GL), and control (C) groups tested at 12 and 24 h. The expression levels of genes in oxidative, apoptotic, autophagic, and necrotic cell death pathways are determined by reverse transcription‐quantitative polymerase chain reaction analysis. The comet assay method is used for the analysis of DNA damage. It is observed that adding lycopene to high glucose for protective purposes reduces the expression of genes related to apoptosis, autophagy, and necrosis, as well as the DNA damage index, compared to cells given high glucose alone. Lycopene can be a safe and effective alternative agent.

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The Effect of Lycopene on DNA Damage and Repair in Fluoride-Treated NRK-52E Cell Line

Cited by 8 publications

References 34 publications

An interventional clinical trial investigating the effects of Spirulina platensis on dental fluorosis and antioxidant system in lambs reared in endemic areas

An interventional clinical trial investigating the effects of Spirulina platensis on dental fluorosis and antioxidant system in lambs reared in endemic areas

Pharmacological potentials of lycopene against aging and aging‐related disorders: A review

Lycopene prevents cell death in NRK‐52E cells by inhibition of high glucose‐activated DNA damage and apoptotic, autophagic, and necrotic pathways

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