The effect of low-intensity pulsed ultrasound on repair of epithelial cell monolayers in vitro

Hill, G.; Fenwick, Steven; Matthews, Bridget J.; Chivers, Robin A.; Southgate, Jennifer

doi:10.1016/j.ultrasmedbio.2005.08.001

Cited by 29 publications

(24 citation statements)

References 26 publications

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“…Enhanced cell replication represents a prerequisite for wound healing and fracture repair stimulated by ultrasound. 3,4,6,7,11,40 Arguably, our explanation for the observed ultrasound enhanced mitotic cycle is not the only possible interpretation, because there could well be other mechanisms, such as influence on DNA-metabolism that lead to the observed effects.…”

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confidence: 85%

Ultrasound‐induced modifications of cytoskeletal components in osteoblast‐like SAOS‐2 cells

Hauser

Muhr

et al. 2009

Journal Orthopaedic Research

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In clinical and experimental studies an acceleration of fracture healing and increased callus formation induced by low-intensity pulsed ultrasound (LIPUS) has been demonstrated. The exact molecular mechanisms of ultrasound treatment are still unclear. In this study ultrasound transmitted cytoskeletal and growth rate changes of SAOS-2 cells were examined. Osteoblast-like cell lines (SAOS-2) were treated using low-intensity pulsed ultrasound. Cytoskeletal changes were analyzed using rhodamine phalloidine for f-actin staining and indirect immunofluorescence techniques with different monoclonal antibodies against several tubulin modifications. To examine changes of cell number after ultrasound treatment cell counts were done. Significant changes in cytoskeleton structure were detected compared to controls, including an enhancement of stress fiber formation combined with a loss of cell migration after ultrasound application. We further observed that sonication altered the proportion of the more stable microtubules to the more labile microtubule subclass. The labile tyrosinated microtubules appeared highly enhanced, whereas the amount of the more stable acetylated microtubules was remarkably diminished. All these observations were quantified by fluorometric measurements. The centrosomal g-tubulin was frequently scattered throughout the cell's cytoplasm, giving rise to additional polyglu-positive microtubular asters, which induced multipolar spindles, leading either to aneuploid mini-or giant cells. Moreover, a significant increase of cell number was noticed in the sonicated group. These experiments demonstrate that ultrasound treatment increases cell number and leads to significant changes of the cytoskeletal structure and composition in vitro. Keywords: ultrasound; bone stimulation; cytoskeletal changes; in vitro study; fracture healing In the course of earlier studies on the elasticity properties of the mitotic spindle of endothelial heart cells from frogs, surprising effects on living interphase and mitotic cells during acoustic imaging at 2.0 giga Hertz were accidentally observed by one of the authors, 1 namely, a drastic acceleration of spindle dynamics and massive de novo formation of stress fiber bundles in interphase cells. Therefore, we assumed that acoustic pulsed energy acts on membrane mechanotransducers transmitting energy into intracellular biochemical signals.Additional studies in the mega Hertz range showed that shear stress can indeed trigger intracellular biochemical reactions.2 Therapeutic low-intensity pulsed ultrasound (LIPUS) enhances bone formation and is used to accelerate fracture healing and treat pseudarthrosis. 3,4 In animal studies, LIPUS enhanced endochondral formation in a dose-dependent way. 5Therefore, LIPUS became a focus in our research. 6,7 We are convinced that ultrasound in a broad range of frequencies and intensities can influence biochemical pathways by inducing signals for enhanced cell proliferation rates. In fact, ultrasound promotes osteogenesis, Ca 2þ uptake, and ...

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mentioning

confidence: 85%

Ultrasound‐induced modifications of cytoskeletal components in osteoblast‐like SAOS‐2 cells

Hauser

Muhr

et al. 2009

Journal Orthopaedic Research

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“…It has been demonstrated that US increases synthesis of collagen by fibroblasts and epithelium repairing. [48][49][50] The last phase of tissue healing process is "remodeling." In addition, there are some proofs that scar tissue cured with US may be more powerful and elastic than "normal" scar tissue.…”

Section: Biological Interactions Of Usmentioning

confidence: 99%

Untitled

Rashidi

2017

IJGP

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Objective: Ultrasound (US) waves have unique characteristics that making them promising option for treatment of different soft tissue injuries particularly wounds. The mechanisms of actions of this technique in the treatment of soft tissue are not fully understood. This paper aimed to comprehensively review the biological interactions and mechanism of action of US waves in the treatment of chronic wounds. Methods: The databases of PubMed (1990PubMed ( -2016, EMBASE (1990EMBASE ( -2016, Web of Sciences (1990), and Google Scholar (1980 were searched using the set terms "US waves" and "wound treatment," "mechanism of action" and "soft tissue injuries," and "biologi8cal interaction." The title and abstract of the collected results were reviewed by two authors, and the relevant papers were selected for further evaluations. Results: The mechanisms of action depend on the US physical parameters as well as exposure factors including duration and injury type. The main mechanisms of US waves for wound healing are enhancing the rate of inflammatory phase, stimulating fibroblasts to secrete collagen, enhancing extensibility of collagen, circulation, pain threshold, enzymatic activity, permeability of cell membrane, and accelerating nerve conduction. Conclusion: The therapeutic effects of US depend on dose (W/cm 2 ) and dosage (frequency of application). Low US frequencies show more therapeutic efficiency in wound healing compared with high frequencies.

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“…The C2C12 cell lineage used in this study is a subclone of the C2 myoblast line, which derives from the SC of adult mice, and presents most characteristics of normal muscle cells [14][15][16] . Cells were cultivated in the Dulbecco's Modified Eagle Medium (DMEM) (Cultilab, Campinas, SP, Brazil), with 10% fetal bovine serum (FBS) (Cultilab, Campinas, SP, Brazil) and 1% antibiotic-antimycotic solution (Cultilab), being maintained in a 37ºC stove, under a humid atmosphere with 5% of CO 2 .…”

Section: Cell Culturementioning

confidence: 99%

Effects of nandrolone decanoate on the viability of muscle satellite cells during the differentiation process

et al. 2014

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| Studies indicate that the anabolic nandrolone decanoate (Deca-Durabolin®) can modulate cell cycle regulation, but little is known about its effects on muscle cells. Anabolic steroids are used, especially by athletes, to improve muscle mass and performance in the practice of exercises. The aim of this study was to evaluate the effect of the anabolic Deca-Durabolin® on the proliferation of skeletal muscle precursor cells C2C12. Cells were grown in Dulbecco's Modified Eagle Medium (DMEM), being supplemented with 10% Fetal Bovine Serum (FBS) and subjected to differentiation by the addition of 2% horse serum. They were incubated with anabolic at concentrations of 5, 10, 25 and 50 µM. The groups that received no anabolic or vehicle served as controls. The viability (proliferation) was evaluated by the MTT method (3-[4,5-Dimethylthiazol-2yl]-2,5-diphenyltetrazolium bromide; Thiazolyl blue) after one, three and five days of incubation. Three independent experiments were performed in each of the mentioned conditions, and the results were submitted to statistical analysis with significance level of p≤0.05 (ANOVA/Dunnett). Results showed no difference in viability between muscle cells treated with anabolic and the control cultures in all parameters. In conclusion, nandrolone, at the used concentrations, was not able to alter the viability of muscle C2C12 satellite cells. Keywords | Anabolic; Athletes; Muscle Cells; Nandrolone; Cell Proliferation; Cell Differentiation.resumo | Os anabolizantes são utilizados, especialmente por atletas, com o intuito de aumento da massa muscular e melhora do desempenho. Este estudo objetivou avaliar o efeito do anabolizante Deca-Durabolin® sobre a viabilidade (proliferação) de células satélites musculares C2C12 induzidas à diferenciação, mimetizando o processo de reparo após lesão. As células foram cultivadas em Meio Eagle Modificado por Dulbecco (DMEM) suplementado com 10% de Soro Fetal Bovino (SFB) e submetidas à diferenciação pela adição de 2% de soro de cavalo e concomitantemente incubadas com o anabolizante nas concentrações de 5, 10, 25 e 50 µM. Os grupos que não receberam o anabolizante nem o veículo serviram como controle. A viabilidade (proliferação) foi avaliada após um, três e cinco dias, utilizando o método de MTT (3-[4,5-dimetiltriazol-2-il]-2,5 difeniltetrazólio). Foram realizados três experimentos independentes, em cada condição citada, e os resultados submetidos à análise estatística com nível de significância de p≤0,05% (ANOVA/Dunnet). Os resultados permitiram verificar que não houve diferença na viabilidade entre células musculares tratadas com o anabolizante e induzidas à diferenciação e as culturas

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The effect of low-intensity pulsed ultrasound on repair of epithelial cell monolayers in vitro

Cited by 29 publications

References 26 publications

Ultrasound‐induced modifications of cytoskeletal components in osteoblast‐like SAOS‐2 cells

Ultrasound‐induced modifications of cytoskeletal components in osteoblast‐like SAOS‐2 cells

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Effects of nandrolone decanoate on the viability of muscle satellite cells during the differentiation process

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