The effect of low doses of zearalenone and its metabolites on progesterone and 17β-estradiol concentrations in peripheral blood and body weights of pre-pubertal female Beagle dogs

Gajęcka, Magdalena; Zielonka, Łukasz; Dąbrowski, Michał; Mroz, Magdalena S.

doi:10.1016/j.toxicon.2013.08.060

Cited by 27 publications

(37 citation statements)

References 34 publications

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“…The samples were immediately transferred to chilled centrifuge tubes containing heparin and were centrifuged at 3000 rpm for 20 minutes at 4 o C. The resulting plasma was placed in 3 ml Eppendorf tubes and freeze-stored at -20 o C until analysis. Gajęcka et al (2013).…”

Section: Blood Sampling and Preparation For Mycotoxin Analysismentioning

confidence: 99%

The effect of selected environmental Fusarium mycotoxins on the ovaries in the female wild boar (Sus scrofa)

Zielonka¹,

Gajęcka²,

Żmudzki³

et al. 2015

Polish Journal of Veterinary Sciences

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The contamination of agricultural crops with Fusarium mycotoxins poses one of the greatest problems in food production. Wild boars live in specific habitats and are physiologically sensitive to Fusarium mycotoxins, therefore, they are an interesting model for studies investigating the effects of the discussed toxin, in particular under low-dose exposure. The objective of this study was to determine potential effects of Fusarium mycotoxins ingested with naturally contaminated food on reproductive function based on the proliferation and apoptotic indices of ovarian follicles in female wild boars. The experiment was conducted on 40 wild boars inhabiting north-eastern Poland. The effect of seasonal variations in the quantity and quality of ingested food on the concentrations of Fusarium mycotoxins and their metabolites in the blood of wild boars was analyzed. The observed differences in toxin levels were accompanied by changes in proliferation and apoptotic indices. Proliferation processes were most intense in autumn-winter and were least advanced in winter-spring. The intensity of apoptotic processes was inversely correlated with proliferation.

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Section: Blood Sampling and Preparation For Mycotoxin Analysismentioning

confidence: 99%

The effect of selected environmental Fusarium mycotoxins on the ovaries in the female wild boar (Sus scrofa)

Zielonka¹,

Gajęcka²,

Żmudzki³

et al. 2015

Polish Journal of Veterinary Sciences

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“…Low doses up to the NOAEL value induce completely different local and overall effects than those observed under exposure to higher doses of mycotoxins (Calabrese 2005, Dobrzyński and Fornalski 2011, Gajęcka et al 2013. The NOAEL value supports the determination of the dose-response relationship where there is no statistically or biologically significant increase in the frequency or intensity of any harmful effects of the analysed substance is observed relative to the control sample.…”

Section: Introductionmentioning

confidence: 52%

“…It has been suggested that medical errors, mostly inadequate hormonal treatment, often cause pathological states of the reproductive system in bitches (Hoffmann et al 1996). Mycotoxins, in particular ZEN which is often present in commercial feed (Zwierzchowski et al 2004, Gajęcka et al 2013, are also potential causative agents of reproductive disorders in female dogs (Freeman et al 2013a, b). Preliminary research revealed differences in ZEN concentrations in commercial feeds, some of which contained substantial amounts of the toxin (Zwierzchowski et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Feed contamination with low doses of the mycotoxin (near to NOAEL values, i.e. the highest dose that does not induce clinical symptoms) is of particular significance for bitches, which are often fed monodiets for many months (Gajęcka et al 2013). ZEN doses below NOAEL values are most often found in commercial feeds (Boermans and Leung 2007, Bohm et al 2010, Schatzmayr and Streit 2013.…”

Section: Introductionmentioning

confidence: 99%

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Immunohistochemical evaluation of apoptosis and proliferation in the mucous membrane of selected uterine regions in pre-pubertal bitches exposed to low doses of zearalenone

Stopa¹,

Babińska²,

Zielonka³

et al. 2016

Polish Journal of Veterinary Sciences

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Mycotoxins are toxic secondary metabolites produced by fungi. Those biologically active compounds occur naturally and they include zearalenone (ZEN), a mycotoxin that contaminates plant material, including the ingredients used in the production of commercial dog food. The influence of monotonic, low-dose and long-term exposure to ZEN on pre-pubertal bitches has not been fully explored to date. This paper describes a 42-day experiment performed on clinically healthy female dogs aged approximately 70 days, with estimated body weight of 8 kg. The animals were randomly divided into two experimental groups (EI and EII) and a control group (C) of 10 animals each. Group EI received 50 μg ZEN/kg (of body weight) per os, group EII received 75 μg ZEN/kg BW per os, and the control group was administered placebo. The bitches were ovariohysterectomized at the end of the experiment (at around 112 days of age), and selected sections of the uterine wall were subjected to immunohistochemical analyses (TUNEL and PCNA). A shift towards higher apoptotic (AI) and proliferative index (PI) was observed, in particular in group EI. Higher AI and PI values were noted in the epithelium of all uterine regions analysed and in the uterine glands in the uterine horn proximal to the body of the uterus.

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“…α-ZEL jest dominującym metabolitem ZEN występującym w organizmie świń, podczas gdy u innych gatunków zwierząt, jak np. u brojlerów, krów i owiec dominującym produktem metabolizmu ZEN jest β-ZEL o dużo mniejszej aktywności metabolicznej (9). Efekt aktywności ZEN zależy od nasilenia i charakteru procesów metabolicznych mających miejsce w organizmie zwierząt (18).…”

Section: Praca Oryginalnaunclassified

Percent changes in mesenteric blood T-lymphocyte subsets in gilts induced by low-dose zearalenone-deoxynivalenol mycotoxin

Dąbrowski

Zielonka

Baranowski

et al. 2017

Medycyna Weterynaryjna

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Środki spożywcze i materiały paszowe pochodzenia roślinnego są bardzo często wektorami niskich dawek mikotoksyn fuzaryjnych (28). Ze względu na szeroki zakres ich zastosowań w przemyśle spożywczym i w produkcji pasz, a także wysoki udział produktów zbożowych w diecie człowieka i zwierząt są ważnym elementem odpowiedzialnym za stan zdrowia ludzi oraz wydajność procesu produkcyjnego zwierząt (10). Najczęściej spotykanymi mikotoksynami fuzaryjnymi są zearalenon (ZEN) oraz deoksyniwalenol (DON).Zearalenon i jego metabolity powodują zaburzenia w rozrodzie zwierząt, ponieważ w swej aktywności są podobne do estradiolu (40). α-ZEL jest dominującym metabolitem ZEN występującym w organizmie świń, podczas gdy u innych gatunków zwierząt, jak np. u brojlerów, krów i owiec dominującym produktem metabolizmu ZEN jest β-ZEL o dużo mniejszej aktywności metabolicznej (9). Efekt aktywności ZEN zależy od nasilenia i charakteru procesów metabolicznych mających miejsce w organizmie zwierząt (18). Zależy również od statusu immunologicznego (5, 20) układu rozrodczego, który podlega ciągłym zmianom na skutek zmian hormonalnych wynikających z okresu dojrzewania płciowego, etapu cyklu rozrodczego czy ciąży. Wykazano, że ZEN może być immunotoksyczny w niskich stężeniach (33). W odpowiedzi na obecność mikotoksyny, wrażliwe komórki przechodzą wiele zmian w zakresie istotnych procesów metabolicznych (proliferacji i różnicowania się komórek, apoptozy, syntezy cząsteczek biologicznie aktywnych, itp.) (37). Pomimo że badania nad ZEN zostały ostatnio zintensyfikowane, to istnieje niewiele badań nad jego wpływem na barierę jelitową i potencjalnego związku pomiędzy ekspozycją na ZEN a stanami zapalnymi jelit (13). Dąbrowski M., Zielonka Ł., Baranowski M., Gajęcka M., Rykaczewska A., Gajęcki M. T.Percent changes in mesenteric blood T-lymphocyte subsets in gilts induced by low-dose zearalenone-deoxynivalenol mycotoxin Mixed low-dose mycotoxin can also be a cause of temporary immunity decline, as well as a factor responsible for disturbances during the maturation of the immune system.

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The effect of low doses of zearalenone and its metabolites on progesterone and 17β-estradiol concentrations in peripheral blood and body weights of pre-pubertal female Beagle dogs

Cited by 27 publications

References 34 publications

The effect of selected environmental Fusarium mycotoxins on the ovaries in the female wild boar (Sus scrofa)

The effect of selected environmental Fusarium mycotoxins on the ovaries in the female wild boar (Sus scrofa)

Immunohistochemical evaluation of apoptosis and proliferation in the mucous membrane of selected uterine regions in pre-pubertal bitches exposed to low doses of zearalenone

Percent changes in mesenteric blood T-lymphocyte subsets in gilts induced by low-dose zearalenone-deoxynivalenol mycotoxin

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